A5007538292- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Viral-associated cancers and disorders
- Plant Virus Research Studies
- interferon and immune responses
- HVDC Systems and Fault Protection
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Toxoplasma gondii Research Studies
- Poxvirus research and outbreaks
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
- Acute Myeloid Leukemia Research
National Institute of Allergy and Infectious Diseases
2021
National Institutes of Health
2021
Children's Hospital of Philadelphia
2017-2020
Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition N6-methyladenosine (m6A) to mRNAs. While m6A-modified adenoviral have been previously detected, location function this mark within infectious cycle unknown. Since complex adenovirus transcriptome includes overlapping spliced units that would impede accurate m6A mapping using short-read sequencing, here we...
Herpes simplex virus (HSV-1) lytic infection results in global changes to the host cell proteome and proteins associated with chromatin. We present a system level characterization of dynamics during by performing multi-dimensional analysis HSV-1 human foreskin fibroblast (HFF) cells. Our study includes identification quantification viral proteomes, phosphoproteomes, chromatin bound proteomes post-translational modifications (PTMs) on cellular histones infection. analyzed across six time...
ABSTRACT Viral manipulation of cellular proteins allows viruses to suppress host defenses and generate infectious progeny. Due the linear double-stranded DNA nature adenovirus genome, damage response (DDR) is considered a barrier successful infection. The genome packaged with protein VII, virally encoded histone-like core that suggested protect incoming viral genomes from detection by machinery. We showed VII localizes chromatin during infection, leading us hypothesize may affect responses...
More than 14,000 neonates are infected with herpes simplex virus (HSV) annually. Approximately half display manifestations limited to the skin, eyes, or mouth (SEM disease). The rest develop invasive infections that spread central nervous system (CNS disease encephalitis) throughout neonate (disseminated Invasive HSV is associated significant morbidity and mortality, but viral host factors predispose these forms unknown. To define diversity within neonatal population, we evaluated 10 HSV-2...
PML nuclear bodies (PML-NBs) are dynamic subnuclear structures important for chromatin dynamics and anti-viral defense. In this study we investigate the role of Sp100 isoforms in promoting localization H3.3 histone chaperone HIRA to PML-NBs human keratinocytes. knockout (KO) cell lines were generated using CRISPR-Cas9 technology shown display normal keratinocyte differentiation PML-NB formation. However, its associated complex members (UBN1 ASF1a) failed localize absence Sp100, even after...
Abstract Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition N6 -methyladenosine (m 6 A) to mRNAs. While m A-modified adenoviral have been previously detected, location function this mark within infectious cycle unknown. Since complex adenovirus transcriptome includes overlapping spliced units that would impede accurate A mapping using short-read sequencing,...
Abstract More than 14,000 neonates are infected with herpes simplex virus (HSV) annually. Approximately half display manifestations limited to the skin, eyes, or mouth (SEM disease). The rest develop invasive infections that spread central nervous system (CNS disease encephalitis) systemically (disseminated Invasive HSV is associated significant morbidity and mortality, but viral host factors predispose these forms unknown. To define diversity within neonatal population, we evaluated ten...