This study evaluated bone and cementum regeneration following periodontal reconstructive surgery using recombinant human morphogenetic protein-2 (rhBMP-2) in six beagle dogs. Surgically created mandibular supraalveolar premolar tooth defects contralateral jaw quadrants were randomly assigned to receive rhBMP-2 or control vehicle. Clinical defect height was prepared 5 mm. applied with synthetic bioerodable particles autologous blood 20 micrograms per 100 microliters implant volume. Flaps...

One objective of periodontal therapy after resolution the infectious process is to facilitate a new connective tissue attachment root dentin surface. Here we report that selective advantage for and growth can be conferred on human gingival fibroblasts by biochemical manipulation Tetracycline HCL treatment surface increases binding fibronectin. The adsorbed fibronectin stimulates fibroblast growth, while suppressing epithelial cell growth. This may provide useful approach periodontally involved teeth.

A wound stabilizing effect of expanded polytetrafluoroethylene (ePTFE) membranes was evaluated in supraalveolar periodontal defects 5 beagle dogs. The defects, to 6 mm height, were surgically created around the 2nd, 3rd, and 4th mandibular premolar teeth contralateral jaw quadrants. root surfaces conditioned with heparin, which, this model, has been demonstrated compromise healing result formation a long junctional epithelium. Wound closure included application ePTFE each tooth one quadrant...

The objective of this study was to evaluate the effect recombinant human bone morphogenetic protein-2 (rhBMP-2) concentration on regeneration alveolar and cementum, associated root resorption ankylosis. Contralateral, critical size, supra-alveolar, periodontal defects were surgically produced immediately implanted with rhBMP-2 in an absorbable collagen sponge (ACS) carrier 8, young adult, male, beagle dogs. 6 animals received rhBMP-2/ACS (rhBMP-2 at 0.05, 0.10, or 0.20 mg/mL; total construct...

S ixteen intrabony defects in 12 patients were treated by gingival flap surgery including root surface debridement and placement of an expanded polytetrafluoroethylene (ePTFE) membrane. The membranes removed after 4 to 6 weeks examined scanning electron microscopy (SEM) for bacterial contamination adherent connective tissue elements. Twelve months postsurgery, the defect sites reexamined changes probing attachment level bone level. Comparison ultrastructural findings clinical observations...

Selective use of tetracycline HCl was studied to evaluate a potential treatment methodology. Tetracycline adsorbed dentin surfaces, binding 4.7 μg/mm 2 after 5 min exposure 50 mg/ml solution. Desorption in discontinuous flow assay maintained biologically active concentrations the fluid phase for at least 48 h. The bound, and subsequently released from retained antimicrobial activity with an ID 3.7 μg/ml. conditioning removed amorphous surface layer, thereby exposing open tubules, as...

Invasion of periodontal tissues by different bacterial morphotypes has been reported in human periodontitis; however, limited information is available as to prevalence, localization and the species involved. The present study determined prevalence gingival Actinobacillus (Haemophilus) actinomycetemcomitans lesions juvenile periodontitis patients. Thirty‐five biopsies were obtained from 12 patients at time therapy. One additional control biopsy was each two adult periodontally healthy...

Abstract The purpose of this retrospective study was to determine factors affecting clinical outcome guided tissue regeneration (GTR) in the treatment intrabony periodontal defects. 38 patients each contributing 1 isolated defect treated with GTR were included analysis. Patient and characteristics, defect‐specific recordings relative 6 months postsurgery assessed. resulted clinically statistically significant improved probing depths (PD), attachment levels (CAL), bone (PBL). Presurgery PD...

Studies using ectopic rodent, orthotopic canine, and non-human primate models show that bone morphogenetic proteins (BMPs) coated onto titanium surfaces induce local formation. The objective of this study was to examine the ability recombinant human BMP-2 (rhBMP-2) a porous oxide implant surface stimulate formation including osseointegration vertical augmentation alveolar ridge.Bilateral, critical-size, 5 mm, supra-alveolar, peri-implant defects were created in 12 young adult Hound Labrador...

Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier is being evaluated as a candidate therapy for periodontal regeneration. The objective of this study was to evaluate regeneration alveolar and cementum, associated root resorption ankylosis following surgical implantation rhBMP-2/ACS canine clinical model.Bilateral 3-wall intrabony defects were surgically induced the premolar region maxilla mandible 8 young adult Korean mongrel dogs. each...

Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) induced regeneration and osseointegration was evaluated in supra‐alveolar peri‐implant defects 5 beagle dogs. Alveolar reduced mm height mandibular premolar areas the premolars were extracted. Three 10 titanium fixtures placed into each alveolar crest, leaving a supraalveolar position. Alternate quadrants consecutive animals received surgical implant consisting of 2 ml rhBMP‐2 (0.43 mg/ml) type I bovine collagen carrier, or carrier...

Abstract Background: It is common belief that immediate implant placement into extraction sites may act to preserve the alveolar process. The objective of this study was evaluate healing dynamics at buccal peri‐implant in relation dimensions ridge. Methods: Bilateral, critical‐size, supraalveolar, defects were created 12 male Hound Labrador mongrel dogs following surgical horizontal cut‐down Each jaw quadrant received three 10‐mm titanium implants placed 5 mm third and fourth premolar teeth...

R egeneration of alveolar bone and cementum following reconstructive therapy with reinforced space providing expanded polytetrafluoroethylene (ePTFE) membranes was evaluated in supraalveolar mandibular premolar periodontal defects five beagle dogs. The surgically‐created contralateral jaw quadrants were randomly assigned to receive the dome‐shaped membrane or serve as surgical control. Flaps positioned completely submerge teeth sutured. dogs sacrificed 8 weeks after surgery tissue blocks...

Previous studies have shown a limited potential for bone augmentation following guided regeneration (GBR) in horizontal alveolar defects. Surgical implantation of recombinant human morphogenetic protein-2 (rhBMP-2) an absorbable collagen sponge carrier (ACS) significantly enhances such defects; however, sufficient quantities implant dentistry are not routinely obtained. The objective this study was to evaluate the rhBMP-2/ACS enhance GBR using space-providing, macro-porous expanded...

Background: The objective of this study was to histologically evaluate periodontal healing following flap surgery in intrabony defects determine the influence number bone walls on regeneration. Methods: One‐, 2‐, and 3‐wall were surgically produced at proximal aspect mandibular premolars either right or left jaw quadrants six beagle dogs. Mucoperiosteal flaps positioned sutured their presurgery position defect preparation. animals euthanized 8 weeks post‐surgery, block sections sites...

Subtraction radiography, a sensitive and accurate technique for identifying alveolar crestal change from standardized pairs of radiographs, is useful in monitoring periodontal therapy. One half the radiographs were found to be appropriate subtraction analysis using present technology taking radiographs. The criterion usability was identical interpretation images made duplicate pair A set analyzed by radiography as well measurement bone‐crest height. more detecting change. Whereas 53% showed...

Recent immunohistological studies have suggested that Actinobacillus actinomycetemcomitans is present in the gingival tissues juvenile periodontitis lesions. The study examined tissue bound A. by bacterial culture and demonstration of antigen tissue. A total 14 lesions were examined. Eleven biopsies obtained from gingiva adjacent to infected pockets, while remaining three control pockets where subgingival infection could not be detected. Each biopsy was hemisected, one half used for...

Background: The single flap approach (SFA) is a minimally invasive procedure designed for periodontal reconstructive procedures of intraosseous defects characterized by dominant unilateral, buccal or oral, extension. This study evaluates the adjunctive effect guided tissue regeneration (GTR) combined with hydroxyapatite (HA) biomaterial in management accessed SFA compared to alone. Methods: Twenty‐four (in 24 patients) were randomly allocated treatment + HA/GTR. Clinical outcomes assessed 6...