A5041531713- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Research on Leishmaniasis Studies
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- Trypanosoma species research and implications
- Rabies epidemiology and control
- Veterinary medicine and infectious diseases
- Molecular Biology Techniques and Applications
- Immune Response and Inflammation
- Toxin Mechanisms and Immunotoxins
- Maternal and Neonatal Healthcare
- Streptococcal Infections and Treatments
- Phytochemical compounds biological activities
- Global Maternal and Child Health
- Antibiotic Resistance in Bacteria
- Synthesis and Biological Evaluation
- Traditional and Medicinal Uses of Annonaceae
- T-cell and Retrovirus Studies
- Complement system in diseases
- Diphtheria, Corynebacterium, and Tetanus
- Marine Invertebrate Physiology and Ecology
- Autoimmune and Inflammatory Disorders Research
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2018-2024
Universidade de São Paulo
2007-2023
Instituto de Medicina Tropical
2008-2020
Instituto de Medicina Avançada
2007-2017
University of California, Irvine
2016
Instituto de Pesquisas Energéticas e Nucleares
2008-2009
National Nuclear Energy Commission
2009
Summary Infection by the protozoan parasite, Toxoplasma gondii is widely prevalent in humans and animals throughout world. Transmission takes place mainly ingestion of raw or undercooked meat that contains parasite cysts oocysts excreted cat faeces, which can contaminate water vegetables. The incidence toxoplasmosis urban areas thus be also related to environmental contamination with oocysts. A direct measure this oocyst counting unfeasible for technical reasons. An interesting alternative...
Visceral leishmaniasis is a fatal parasitic neglected disease affecting 1.5 million people worldwide. Based on drug repositioning approach, the aim of this work was to investigate in vitro immunomodulatory potential buparvaquone (BPQ) and establish safe regimen evaluate vivo efficacy BPQ entrapped by negatively charged nanoliposomes (BPQ-LP) Leishmania infantum-infected hamsters. Small-angle X-ray scattering, dynamic light ζ-potential were applied order study influence liposome structure....
Synthetic analogues of marine sponge guanidine alkaloids showed in vitro antiparasitic activity against Leishmania (L.) infantum and Trypanosoma cruzi. Guanidines 10 11 presented the highest selectivity index when tested Leishmania. The was investigated host cells parasites. Both compounds induced depolarization mitochondrial membrane potential, upregulation reactive oxygen species levels, increased plasma permeability Immunomodulatory assays suggested an NO-independent effect guanidines on...
Toxoplasma gondii is an obligate intracellular parasite that infects a variety of mammals and birds. T. also causes human toxoplasmosis; although toxoplasmosis generally benign disease, ocular, congenital or reactivated disease associated with high numbers disabled people. Infection occurs orally through the ingestion meat containing cysts by intake food water contaminated oocysts. Although immune system responds to acute infection mediates clearance tachyzoites, persist for lifetime host in...
Leishmaniasis is a neglected disease that affects more than 12 million people, with limited therapy. Plant-derived natural products represent useful source of anti-protozoan prototypes. In this work, four derivatives were prepared from neolignans isolated the Brazilian plant Nectandra leucantha, and their effects against intracellular amastigotes Leishmania (L.) infantum evaluated in vitro. IC50 values between 6 35 µM observed silico predictions suggested good oral bioavailability, no PAINS...
Abstract This study comprised the application of a survey in São Paulo, Brazil, 2 different periods 2020: beginning covid-19 pandemic and disease’s first peak (from March to April, 100 interviews) time stability case fatality rates May July, interviews); questionnaire included was composed 14 multiple-choice questions evaluate importance mass communication channels, including social media, level attributed preventive measures at pandemic. The changes people’s behavior, even group with more...
Liposomes containing phosphatidylserine (PS) has been used for the delivery of drugs into intramacrophage millie. Leishmania (L.) infantum parasites live inside macrophages and causes a fatal neglected viscerotropic disease, with toxic treatment. Sertraline was studied as free formulation (SERT) also entrapped phosphatidylserine-liposomes (LP-SERT) against intracellular amastigotes in murine model visceral leishmaniasis. LP-SERT showed potent activity an EC50 value 2.5 μM. The vivo efficacy...
Toxoplasmosis is one of the most prevalent parasitic infections man and livestock, its transmission has usually been attributed to ingestion undercooked or raw meat from infected with infection rate in those animals being an important risk predictor human disease, high Brazil São Paulo State.Looking for this public health problem, we tested serum samples cattle, goat, sheep chicken State Paulo, Brazil, IgG antibodies Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA).Antibodies...
Background The leishmanicidal action of tricyclic antidepressants has been studied and evidences have pointed that their is linked to inhibition trypanothione reductase, a key enzyme in the redox metabolism pathogenic trypanosomes. Cyclobenzaprine (CBP) structurally related antidepressant amitriptyline, differing only by presence double bond central ring. This paper describes effect CBP experimental visceral leishmaniasis, its inhibitory reductase potential immunomodulatory activity....
Infections caused by multidrug-resistant A. baumannii are a worldwide health concern with high mortality rates. Rapid identification of this infectious agent is critical as it can easily spread difficult or no options for treatment. In context, the development reliable and economically viable detection therapeutic methodologies still challenging. One promising solutions nucleic acid aptamers capable interacting bacteria. These be used specific recognition agents well blocking their...
Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein.The n-hexane extract twigs N. were subjected to HPLC/HRESIMS...
El presente trabajo se realizó en Sao Paulo, Brasil con la finalidad de establecer frecuencia infección toxoplásmica cerdos destinados al consumo humano. Mediante prueba ELISA, analizaron 303 sueros suinos beneficiados un camal ciudad. Los resultados dieron 9.57% reactores Toxoplasma gondii (cutoff 0.270) intervalo confianza 95% entre 6.26% y 12.88%. Al analizarse variable sexo, encontró 10.31 % infectadas las hembras 9.22% los machos (P> 0,05). Estos hallazgos confirman importancia que...
The study aimed to estimate the level of agreement between two serological diagnosis tests for toxoplasmosis in swine. ELISA and Indirect Hemagglutination Test were evaluated using blood sera collected slaughterhouses from 303 pigs Sao Paulo, Brazil 93 Lima, Peru. Kappa coefficient on Brazilian was 26.0% confidence interval at 95% 7.7 - 44.3% (P <0.05), whereas Peruvian 36.6% 18.0 55.3% <0.01). Besides this, when considering test as reference test, had a co-positive reaction 48.3%...
Plasmodium malariae is the third most prevalent human malaria-causing species and has a patchy, but ample distribution in world. Humans can host parasite for years without presenting significant symptoms, turning its diagnosis control into difficult task. Here, we investigated immunogenicity of recombinant proteins P. MSP1.Five regions PmMSP1 were expressed Escherichia coli as GST-fusion immunized BALB/c mice. The specificity, subtyping, affinity raised antibodies evaluated by enzyme-linked...
We detected Toxoplasma gondii oocysts in feces of experimentally infected cats, using a Kato Katz approach with subsequent Kinyoun staining. Animals serologically negative to T. were orally 5x10² mice brain cysts ME49 strain. Feces collected daily from the 3rd 30th day after challenge. Oocysts by qualitative sugar flotation and quantitative modified stained (KKK). In 7th 15th through technique, but found KKK 6th 16th day, being sensitive for larger period, permanent documentation. The peak...
Purpose: Protein irradiation causes aggregation, chain breakage, and oxidation, enhancing its uptake by antigen-presenting cells. To evaluate if irradiated proteins participate on the protection, we studied immune response induced in mice immunized with soluble extracts of T. gondii tachyzoites (STag) or intact RH (RH0.25 kGy).Material Methods: Soluble Toxoplasma were at different dose Cobalt-60 source. By polyacrylamide gel electrophoresis (SDS-Page) evaluated effects primary structures...
HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) belongs to group of proteins induced under stress situation, and it has close relation with the nervous system, especially in retina. Toxoplasma gondii causes ocular toxoplasmosis been associated late manifestation congenital although experimental models show morphological alterations are already present during embryological development. Here, we used 18 eyes Gallus domesticus embryos 7th 20th embryonic days establish model...