A5060655011- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Cancer Genomics and Diagnostics
- Redox biology and oxidative stress
- Heat shock proteins research
- Privacy-Preserving Technologies in Data
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Scientific Computing and Data Management
- RNA modifications and cancer
- Extracellular vesicles in disease
- Prostate Cancer Treatment and Research
- Data Quality and Management
- Animal Genetics and Reproduction
Wellcome Sanger Institute
2017-2020
University of Cambridge
2013-2018
Cancer Research UK
2013-2018
Genomics England
2013
Alzheimer’s Research UK
2011
The mammalian radiation has corresponded with rapid changes in noncoding regions of the genome, but we lack a comprehensive understanding regulatory evolution mammals. Here, track promoters and enhancers active liver across 20 species from six diverse orders by profiling genomic enrichment H3K27 acetylation H3K4 trimethylation. We report that is universal feature genomes. Most recently evolved arise ancestral DNA exaptation, rather than lineage-specific expansions repeat elements. In...
Understanding the mechanisms driving lineage-specific evolution in both primates and rodents has been hindered by lack of sister clades with a similar phylogenetic structure having high-quality genome assemblies. Here, we have created chromosome-level assemblies Mus caroli pahari genomes. Together musculus Rattus norvegicus genomes, this set rodent genomes is divergence times to Hominidae (human-chimpanzee-gorilla-orangutan). By comparing evolutionary dynamics between Muridae Hominidae,...
We have previously identified peroxiredoxin-3 (PRDX-3) as a cell-surface protein that is androgen regulated in the LNCaP prostate cancer (PCa) cell line. PRDX-3 member of peroxiredoxin family are responsible for neutralising reactive oxygen species. expression was examined tissue from 32 patients using immunohistochemistry. Subcellular distribution determined confocal microscopy. antiandrogen-resistant lines by western blotting and quantitative RT–PCR. The pathways overexpression knockdown...
ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, applied it to five proteins in mouse livers single 96-well plate, demonstrating extremely high degree qualitative and quantitative reproducibility among biological technical replicates. We estimated optimum minimum recommended cell numbers required...
ABSTRACT Understanding the mechanisms driving lineage-specific evolution in both primates and rodents has been hindered by lack of sister clades with a similar phylogenetic structure having high-quality genome assemblies. Here, we have created chromosome-level assemblies Mus caroli pahari genomes. Together musculus Rattus norvegicus genomes, this set rodent genomes is divergence times to Hominidae (human-chimpanzee-gorilla-orangutan). By comparing evolutionary dynamics between Muridae...
The extensive and continuous reuse of sensitive health data could enhance the role population research on public decisions. This paper describes design principles different building blocks that have supported implementation deployment Population Health Information Research Infrastructure (PHIRI), strengths challenges approach some future developments.