A5007527973- Immune cells in cancer
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Molecular Junctions and Nanostructures
- Dendrimers and Hyperbranched Polymers
- Advanced Biosensing Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Biosensors and Analytical Detection
- Surface Chemistry and Catalysis
- Electrocatalysts for Energy Conversion
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Organic Electronics and Photovoltaics
- Catalytic Cross-Coupling Reactions
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Organic Light-Emitting Diodes Research
- Single-cell and spatial transcriptomics
- Synthetic Organic Chemistry Methods
- Advanced Fluorescence Microscopy Techniques
University of Geneva
2023
Center for Systems Biology
2019-2023
Harvard University
2021-2023
Massachusetts General Hospital
2019-2021
University of Wisconsin–Madison
2015-2018
There is a need for novel analytical techniques to study the composition of single extracellular vesicles (EV). Such are required improve understanding heterogeneous EV populations, allow identification unique subpopulations, and enable earlier more sensitive disease detection. Because small size their low protein content, ultrahigh sensitivity technologies required. Here, an immuno-droplet digital polymerase chain reaction (iddPCR) amplification method described that allows multiplexed...
The action of molecular catalysts comprises multiple microscopic kinetic steps whose nature is central importance in determining catalyst activity and selectivity. Single-molecule microscopy enables the direct examination these steps, including elucidation molecule-to-molecule variability. Such diversity particularly important for behavior supported at surfaces. We present first combined investigation initiation dynamics an operational palladium cross-coupling bulk single-molecule levels,...
Macrophages often abound within tumors, express colony-stimulating factor 1 receptor (CSF1R), and are linked to adverse patient survival. Drugs blocking CSF1R signaling have been used suppress tumor-promoting macrophage responses; however, their mechanisms of action remain incompletely understood. Here, we assessed the lung tumor immune microenvironment in mice treated with BLZ945, a prototypical small-molecule inhibitor, using single-cell RNA sequencing mechanistic validation approaches. We...
The interplay between micromorphology and electronic properties is an important theme in organic materials. Here, we show that a spirofluorene-functionalized boron-dipyrromethene (BODIPY) with alkyl norbornyl tail self-assembles into nanoparticles qualitatively different as compared to the polymerized species. Further, exhibit host of unique emissive properties, including photobrightening, blue satellite peak, spectral diffusion. Extensive photophysical characterization, single-particle...
A series of surface-supported molecular palladium catalysts were synthesized using a dendrimeric attachment motif to incorporate multiple BODIPY fluorophores for single-molecule fluorescence microscopy. An unusual intensity scaling law was observed, whereby the addition did not result in substantial increase brightness. Possible quenching mechanisms are discussed, and simulations photophysical population dynamics used identify singlet–triplet annihilation as likely origin law. This work is...
Disulfide rebridging methods have recently emerged as a route to hinge region-specific antibody modification, and there exist numerous examples of successful chemistry applied clinically relevant human IgG1 antibodies. Here, dibromopyridazinedione disulfide is adapted fast trans-cyclooctene/tetrazine (TCO/Tz) bioorthogonal ligations extended beyond therapeutic antibodies for the first time include mouse rat monoclonal integral multiplexed analytical diagnostics. In spite common architecture,...
Emerging point-of-care diagnostic tests capable of analyzing whole mammalian cells often rely on the attachment harvested to glass surfaces for subsequent molecular characterization. We set out develop and optimize a kit diagnosis lymphoma in low- middle-income countries where access advanced healthcare testing is absent or prone error. Here, we optimized process lyophilization neutravidin-coated cocktails antibodies relevant establish long-term stability reagents required cell capture...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionCorrection to “Fluorescent Dendrimeric Molecular Catalysts Demonstrate Unusual Scaling Behavior at the Single-Molecule Level”Sunil P. Upadhyay, Katherine M. Lupo, Angela N. Marquard, James D. Ng, Desiree Bates, and Randall H. Goldsmith*Cite this: J. Phys. Chem. C 2015, 119, 44, 25134Publication Date (Web):October 22, 2015Publication History Published online22 October 2015Published inissue 5...
<div>Abstract<p>Macrophages often abound within tumors, express colony-stimulating factor 1 receptor (CSF1R), and are linked to adverse patient survival. Drugs blocking CSF1R signaling have been used suppress tumor-promoting macrophage responses; however, their mechanisms of action remain incompletely understood. Here, we assessed the lung tumor immune microenvironment in mice treated with BLZ945, a prototypical small-molecule inhibitor, using single-cell RNA sequencing...
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
<div>Abstract<p>Macrophages often abound within tumors, express colony-stimulating factor 1 receptor (CSF1R), and are linked to adverse patient survival. Drugs blocking CSF1R signaling have been used suppress tumor-promoting macrophage responses; however, their mechanisms of action remain incompletely understood. Here, we assessed the lung tumor immune microenvironment in mice treated with BLZ945, a prototypical small-molecule inhibitor, using single-cell RNA sequencing...
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells
Supplementary Data from Macrophage-Targeted Therapy Unlocks Antitumoral Cross-talk between IFNγ-Secreting Lymphocytes and IL12-Producing Dendritic Cells