A5044457209- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Maternal and fetal healthcare
- Bacterial Genetics and Biotechnology
- Ruminant Nutrition and Digestive Physiology
- Maternal and Perinatal Health Interventions
- Grief, Bereavement, and Mental Health
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- Reproductive Physiology in Livestock
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genetic and phenotypic traits in livestock
- Congenital Anomalies and Fetal Surgery
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Delphi Technique in Research
- Drug-Induced Hepatotoxicity and Protection
- Hip and Femur Fractures
- Health and Conflict Studies
- Pregnancy and preeclampsia studies
- Fetal and Pediatric Neurological Disorders
Royal London Hospital
2010-2025
University of Birmingham
2025
Barts Health NHS Trust
2016-2019
Umeå University
2011-2014
University of Vermont
2004-2011
University of Alberta
2008
Harris Birthright Research Centre for Fetal Medicine
2004
King's College Hospital
2004
Abstract Objective To update the likelihood ratio for trisomy 21 in fetuses with absent nasal bone at 11–14‐week scan. Methods Ultrasound examination of fetal profile was carried out and presence or absence noted immediately before karyotyping 5918 11 to 13+6 weeks. Logistic regression analysis used examine effect maternal ethnic origin crown–rump length (CRL) nuchal translucency (NT) on incidence chromosomally normal fetuses. Results The successfully examined 5851 (98.9%) cases. In...
To develop a set of core outcomes to be minimally reported in trials on induction labour.Two-round Delphi survey and consensus meeting.Four stakeholder groups: midwives, obstetricians, neonatologists, women's representatives.Protocol registered with COMET (Registration Number: 695). Stakeholders rated for importance (1-limited 9-critical). The median rating each outcome was calculated. criteria include were as follows: ≥70% participants critical <15% limited importance. Outcomes that did not...
Thymine glycol (Tg) is a common product of oxidation and ionizing radiation, including that used for cancer treatment. Although Tg poor mutagenic lesion, it has been shown to present strong block both repair replicative DNA polymerases. The 2.65-A crystal structure binary complex the RB69 polymerase with shows templating intrahelical forms regular Watson-Crick base pair incorporated A. C5 methyl group protrudes axially from ring damaged pyrimidine hinders stacking adjacent 5' template...
The biological role of human DNA polymerase θ (POLQ) is not yet clearly defined, but it has been proposed to participate in several cellular processes based on its translesion synthesis capabilities. POLQ a low-fidelity capable efficient bypass blocking lesions such as abasic sites and thymine glycols well extension mismatched primer termini. Here, we show that possesses activity appears be template independent allows single-stranded duplex with either protruding or multiply 3'-OH We...
Excessive haemorrhage at cesarean section requires donor (allogeneic) blood transfusion. Cell salvage may reduce this requirement.We conducted a pragmatic randomised controlled trial (at 26 obstetric units; participants recruited from 4 June 2013 to 17 April 2016) of routine cell use (intervention) versus current standard care without (control) in among women risk haemorrhage. Randomisation was stratified, using random permuted blocks variable sizes. In an intention-to-treat analysis, we...
Background Caesarean section is associated with blood loss and maternal morbidity. Excessive requires transfusion of donor (allogeneic) blood, which a finite resource. Cell salvage returns lost during surgery to the mother. It may avoid need for transfusion, but reliable evidence its effects lacking. Objectives To determine if routine use cell caesarean in mothers at risk haemorrhage reduces rates postpartum morbidity, cost-effective, comparison standard practice without use. Design...
Background Vaginal cervical cerclage and progesterone are established treatments for prevention of pregnancy loss prematurity. There is limited data to assess the effect these in combination. Objective To investigate association between no treatment on outcomes women at high risk preterm birth who have received a vaginal cerclage. Study Design This secondary analysis recruited CSTICH trial, which 75 obstetric units UK. The primary outcome was loss, defined as miscarriage perinatal mortality,...
Background We investigated the impact of Maternal Medicine Network Hub, established at our centre in 2019, on post-partum haemorrhage (PPH) management women with inherited bleeding disorders (IBDs). Methods Data from 63 patients IBDs between 2020 and 2023 were compared to a general obstetric cohort. Specific data collected included haemoglobin levels, estimated blood loss, PPH severity. Results Post-partum rates above 500 mL 1000 significantly higher IBD group ( p = .02 .002, respectively)....
The recent demonstration of detectable circulating fetal RNA in maternal plasma (1)(2) has led to the development new, noninvasive prenatal diagnostic opportunities (3)(4). Unlike DNA measurements plasma/serum, quantitative analysis advantage being applicable all pregnant women irrespective gender and genetic polymorphism status. In addition, unexpected stability enhanced practicality this approach (2)(5)(6). Previously, we developed a real-time reverse transcription-PCR (RT-PCR) assay for...
For DNA polymerases to proofread a misincorporated nucleotide, the terminal 3−4 nucleotides of primer strand must be separated from template before being bound in exonuclease active center. Genetic and biochemical studies bacteriophage T4 polymerase revealed that prominent β-hairpin structure domain is needed efficiently form strand-separated complexes. We present here further mutational analysis loop region structure, which provides additional evidence residues loop, namely, Y254 G255, are...
The fidelity of DNA replication is under constant threat from the formation lesions within genome. Oxidation bases leads to altered such as 8-oxo-7,8-dihydroguanine, commonly called 8-oxoG, and 2-hydroxyadenenine, or 2-OHA. In this work we have examined incorporation kinetics opposite these two oxidatively derived well an abasic site analogue by replicative polymerase bacteriophage RB69. We compared kinetic parameters for both wild type low L561A variant. While nucleotide rates (kpol) were...
The kinetics of forming a proper Watson–Crick base pair as well incorporating bases opposite furan, an abasic site analog, have been characterized for the B Family replicative DNA polymerase from bacteriophage T4. Structural studies these reactions, however, only performed with homologous enzyme RB69. In this work, enzymes RB69 and T4 were compared in parallel reactions to determine relative abilities two polymerases incorporate correct nucleotides form improper pairings. kinetic rates three...
Key content ST analysis ( STAN ) involves a combination of fetal heart rate interpretation and the electrocardiogram. has been available for over 5 years in UK is used some, but not all, maternity units. aims to reduce incidence acidaemia hypoxia by identifying these earlier with greater sensitivity than cardiotocography CTG alone. Learning objectives To be familiar background theory evidence use . learn principles trace. review practical tips application monitors. Ethical issues currently...
Objectives To evaluate the cost-effectiveness of routine use cell salvage during caesarean section in mothers at risk haemorrhage compared with current standard care. Design Model-based evaluation alongside a multicentre randomised controlled trial. Three main analyses were carried out on trial data: (1) based intention-to-treat principle; (2) per-protocol (3) only participants who underwent an emergency section. Setting 26 obstetric units UK. Participants 3028 women recruited between June...
Abstract Background Mother-to-baby transmission of group B Streptococcus (GBS) is the main cause early-onset infection. We evaluated whether, in women with clinical risk factors for early neonatal infection, use point-of-care rapid intrapartum test to detect maternal GBS colonisation reduces antibiotic exposure compared usual care, where antibiotics are administered due those factors. assessed accuracy diagnosing colonisation, against reference standard selective enrichment culture. Methods...
Women decline blood products in pregnancy for religious reasons or because of concerns regarding their safety. Mortality is significantly increased pregnant women who products. Antenatal counselling and planning must involve a senior multidisciplinary team. Considerable efforts are needed to optimise pre‐delivery haemoglobin identify risk factors haemorrhage. Intrapartum techniques avoid transfusion include cell salvage early recourse definitive surgical management the event massive...