A5033413179- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neurotransmitter Receptor Influence on Behavior
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Cannabis and Cannabinoid Research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Nuclear Receptors and Signaling
- Nerve injury and regeneration
- Functional Brain Connectivity Studies
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Genetics and Neurodevelopmental Disorders
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Advanced MRI Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Advanced Memory and Neural Computing
- Memory and Neural Mechanisms
- Sleep and Wakefulness Research
- Nuclear Structure and Function
- Carbon and Quantum Dots Applications
- Congenital heart defects research
Italian Institute of Technology
2015-2025
Instituto de Salud Carlos III
2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2023
Consejo Superior de Investigaciones Científicas
2023
University College London
2012-2023
Karolinska Institutet
2023
Instituto Cajal
2023
Sapienza University of Rome
2001-2006
Istituto Pasteur
2004
University of Milan
1997-2002
Abstract Postmortem studies have revealed increased density of excitatory synapses in the brains individuals with autism spectrum disorder (ASD), a putative link to aberrant mTOR-dependent synaptic pruning. ASD is also characterized by atypical macroscale functional connectivity as measured resting-state fMRI (rsfMRI). These observations raise question whether excess causes ASD. Using rsfMRI, electrophysiology and silico modelling Tsc2 haploinsufficient mice, we show that spine associated...
Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some can exist with at least two stable conformations. Recently, class of intracellular chloride ion channel (CLICs) has been shown to in both soluble and integral membrane forms. The structure the form CLIC1 typical glutathione S-transferase superfamily protein but contains glutaredoxin-like active site. In this study we show on oxidation undergoes reversible transition from monomeric...
CLIC1 (NCC27) is a member of the highly conserved class chloride ion channels that exists in both soluble and integral membrane forms. Purified can integrate into synthetic lipid bilayers forming channel with similar properties to those observed <i>in vivo</i>. The structure form has been determined at 1.4-Å resolution. protein monomeric structurally homologous glutathione<i>S</i>-transferase superfamily, it redox-active site resembling glutaredoxin. complex glutathione shows occupies site,...
Human genetic studies are rapidly identifying variants that increase risk for neurodevelopmental disorders. However, it remains unclear how specific mutations impact brain function and contribute to neuropsychiatric risk. Chromosome 16p11.2 deletion is one of the most common copy number variations in autism related Using resting state functional MRI data from Simons Variation Individuals Project (VIP) database, we show carriers exhibit impaired prefrontal connectivity, resulting weaker...
NCC27 is a nuclear chloride ion channel, identified in the PMA-activated U937 human monocyte cell line. mRNA expressed virtually all cells and tissues gene encoding also highly conserved. Because of these factors, we have examined hypothesis that involved cycle regulation. Electrophysiological studies Chinese hamster ovary (CHO-K1) indicated conductance varied according to stage cycle, being only on plasma membrane G2/M phase. We demonstrate Cl- channel blockers known block led arrest CHO-K1...
Serotonin-producing neurons profusely innervate brain regions via long-range projections. However, it remains unclear whether and how endogenous serotonergic transmission specifically influences regional or global functional activity. We combined designed receptors exclusively activated by drugs (DREADD)-based chemogenetics magnetic resonance imaging (fMRI), an approach we term "chemo-fMRI," to causally probe the brain-wide substrates modulated describe generation of a conditional knockin...
Despite extensive research into understanding synaptic mechanisms of striatal plasticity, the functional role played by astrocytes in this region remains to be fully elucidated. It was recently demonstrated that high-frequency stimulation (HFS) cortical inputs induced long-term depression (LTD) mediated adenosine A1 receptor (A1R) activation at corticostriatal synapses direct pathway [cortico-striatal projection neuron (dSPN)] dorsolateral striatum (DLS). Because astrocyte-derived has been...
CLIC1 (NCC27) is an unusual, largely intracellular, ion channel that exists in both soluble and membrane-associated forms. The recombinant protein can be expressed <i>Escherichia coli</i>, a property has made possible detailed electrophysiological studies lipid bilayers examination of the mechanism membrane integration. Soluble<i>E. coli</i>-derived moves from solution into artificial forms chloride-selective channels with essentially identical conductance, pharmacology, opening closing...
Abstract Cell signaling is central to neuronal activity and its dysregulation may lead neurodegeneration cognitive decline. Here, we show that selective genetic potentiation of ERK prevents cell death in vitro vivo the mouse brain, while attenuation does opposite. This neuroprotective effect mediated by an enhanced nuclear can also be induced novel penetrating peptide RB5. In administration RB5 disrupts preferential interaction ERK1 MAP kinase with importinα1/KPNA2 over ERK2, facilitates...
Animals often behave repetitively and predictably. These repetitive behaviors can have a component that is learned ingrained as habits, which be evolutionarily advantageous they reduce cognitive load the expenditure of attentional resources. Repetitive also conscious deliberate, may occur in absence habit formation, typically when are feature normal development children, or neuropsychiatric disorders. They considered pathological interfere with social relationships daily activities. For...
Parkinson’s disease (PD) is characterized by motor impairments caused degeneration of dopamine neurons in the substantia nigra pars compacta. In addition to these symptoms, PD patients often suffer from non-motor comorbidities including sleep and psychiatric disturbances, which are thought depend on concomitant alterations serotonergic noradrenergic transmission. A primary locus dorsal raphe nucleus (DRN), providing brain-wide input. Here, we identified electrophysiological morphological...
NCC27 belongs to a family of small, highly conserved, organellar ion channel proteins. It is constitutively expressed by native CHO-K1 and dominantly localized the nucleus nuclear membrane. When cells are transfected with NCC27-expressing constructs, synthesized proteins spill over into cytoplasm activity can then be detected on plasma as well This provided unique opportunity directly compare electrophysiological characteristics one cloned channel, both cytoplasmic membranes. At same time,...
Graphical Abstract figure legend HCN channels play an evolutionarily conserved pacemaker role in renal pelvic smooth muscle (RPSM) of lower and higher order mammals. The function hyperpolarization-activated cation (HCN) pacemakers remains controversial. Renal trigger contractions that expel waste from the kidney, have been localized to these tissues. To date, however, mechanisms underlying RPSM activity remain elusive. was investigated both (top left) (bottom mammalian models, which exhibit...
Highlights d eCBs govern LTD in striatopallidal neurons and adenosine does so striatonigral cells Parkinson's disease (PD)-like dopamine (DA) depletion impairs eCB-
Creutzfeldt–Jakob disease (CJD) is a neurodegenerative disorder caused by prion protein (PrP) misfolding, clinically recognized cognitive and motor deficits, electroencephalographic abnormalities, seizures. Its neurophysiological bases are not known. To assess the potential involvement of NMDA receptor (NMDAR) dysfunction, we analyzed NMDA-dependent synaptic plasticity in hippocampal slices from Tg(CJD) mice, which model genetic form CJD. Because PrP depletion may result functional...