A5039223548- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Echinoderm biology and ecology
- Microbial Community Ecology and Physiology
- Aquatic life and conservation
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
University of Wisconsin–Madison
2022-2024
Wisconsin Institutes for Discovery
2022
University of Puerto Rico at Río Piedras
2021
Bacterial secondary metabolites are a major source of antibiotics and other bioactive compounds. In microbial communities, these molecules can mediate interspecies interactions responses to environmental change. Despite the importance in human health ecology, little is known about their roles regulation context multispecies communities. simplified model rhizosphere composed Bacillus cereus , Flavobacterium johnsoniae Pseudomonas koreensis we show that dynamics metabolism depend on community...
CRISPRi (Clustered Regularly Interspaced Palindromic Repeats interference) is a gene knockdown method that uses deactivated Cas9 protein (dCas9) binds specific target locus dictated by an encoded guide RNA (sgRNA) to block transcription. Mobile-CRISPRi suite of modular vectors enable knockdowns in diverse bacteria integrating IPTG-inducible
Abstract CRISPRi (Clustered Regularly Interspaced Palindromic Repeats interference) is a gene knockdown method that uses deactivated Cas9 protein (dCas9) binds specific target locus dictated by an encoded guide RNA (sgRNA) to block transcription. Mobile-CRISPRi suite of modular vectors enable knockdowns in diverse bacteria integrating IPTG-inducible dcas9 and sgRNA genes into the genome using Tn 7 transposition. Here, we show system functions robustly specifically multiple Vibrio species:...
The diversity, ubiquity, and significance of microbial communities is clear. However, the predictable reliable manipulation microbiomes to impact human, environmental, agricultural health remains a challenge.
The increased antibiotics usage in biomedical and agricultural settings has been well documented. Antibiotics have now shown to exert effects outside their purposive use, including on physiological developmental processes. We explored the effect of various intestinal regeneration sea cucumber Holothuria glaberrima. For this, holothurians were eviscerated left regenerate for 10 days seawater with different penicillin/streptomycin-based cocktails (100 µg/mL PS) including: 100 kanamycin (KPS),...
Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, all components integrated into the chromosome via Tn