A5073315946- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Insect symbiosis and bacterial influences
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Antioxidant Activity and Oxidative Stress
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- Pancreatic function and diabetes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Circadian rhythm and melatonin
- Bacterial biofilms and quorum sensing
- Dietary Effects on Health
- Mast cells and histamine
- Adipokines, Inflammation, and Metabolic Diseases
- Melanoma and MAPK Pathways
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- Biomedical Ethics and Regulation
- Cardiovascular Function and Risk Factors
- Ferroptosis and cancer prognosis
University of Utah
2023
Binghamton University
2022
University of California, Los Angeles
2020-2021
Loyola Marymount University
2021
Banaras Hindu University
2021
Dartmouth–Hitchcock Medical Center
2019-2020
Dartmouth College
2020
Jackson Laboratory
2017-2019
University of New Hampshire
2012-2017
Acadia Pharmaceuticals (United States)
2007
Abstract The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing the full only recently become realistic prospect. Here, we use in silico and experiments to critically re-evaluate potential provide taxonomic resolution at species strain level. We demonstrate that targeting variable regions with short-read platforms cannot achieve afforded by entire (~1500 bp) gene. further full-length are sufficiently accurate resolve subtle...
Abstract Type 2 diabetes mellitus (T2D) is a growing health problem, but little known about its early disease stages, effects on biological processes or the transition to clinical T2D. To understand earliest stages of T2D better, we obtained samples from 106 healthy individuals and with prediabetes over approximately four years performed deep profiling transcriptomes, metabolomes, cytokines, proteomes, as well changes in microbiome. This rich longitudinal data set revealed many insights:...
Changes in diet and exercise can alter the gut microbiome of humans mice; however, few studies to date have assessed microbiomes highly fit athletes. In this pilot study, we used metagenomic whole genome shotgun (mWGS) metatranscriptomic (RNA-Seq) sequencing show what organisms are both present active professional amateur level competitive cyclists determine if any significant differences exist between these two groups. Using mWGS data, showed that 33 split into three taxonomic clusters,...
Parallel to major changes in fatty acid and glucose metabolism, defect branched-chain amino (BCAA) catabolism has also been recognized as a metabolic hallmark potential therapeutic target for heart failure. However, BCAA catabolic enzymes are ubiquitously expressed all cell types systemic is manifested disorder associated with obesity diabetes. Therefore, it remains be determined the cell-autonomous impact of cardiomyocytes intact hearts independent from its global effects. In this study, we...
ABSTRACT The physiology of a newly recognized Serratia species, termed South African Caenorhabditis briggsae Isolate (SCBI), which is both nematode mutualist and an insect pathogen, was investigated compared to that marcescens Db11, broad-host-range pathogen. two strains had comparable levels virulence for Manduca sexta similar cytotoxic activity patterns, but motility lipase hemolytic activities differed significantly between them.
A newly recognized Serratia species, termed South African Caenorhabditis briggsae isolate (SCBI), is both a mutualist of the nematode KT0001 and pathogen lepidopteran insects. sp. strain SCBI displays high proteolytic activity, because secreted proteases are known virulence factors for many pathogens, purpose this study was to identify genes essential extracellular protease activity in determine what role play insect pathogenesis cytotoxicity. bank 2,100 transposon mutants generated, six...
GIMAPs are recognized as an important regulator in the carcinogenesis and development of lung cancer, but function GIMAP4 tumor microenvironment (TME) cancers is unclear. In this study, we investigated expression variation adenocarcinoma (LUAD), to explore its association with infiltration immune cells. The Cancer Genome Atlas (TCGA) data Gene Expression Omnibus (GEO) were analyzed. Infiltration cells was identified TIMER (Tumor Immune Estimation Resource) TISIDB (an integrated repository...
Hemolysins are important virulence factors for many bacterial pathogens, including Serratia marcescens The role of the major hemolysin gene in insect pathogen sp. strain SCBI was investigated using both forward and reverse-genetics approaches. Introduction into Escherichia coli resulted a gain hemolytic activity. Inactivation this loss hemolysis but did not attenuate insecticidal Unexpectedly, inactivation significantly increased motility antimicrobial Reverse transcription-quantitative PCR...
Histamine H3 receptors (H3R) regulate the release of histamine and other neurotransmitters in brain. H3R have been shown to modulate many functions, including feeding, sleep, cognition, making them attractive drug targets. We developed a functional cell-based assay, R-SAT® (Receptor Selection Amplification Technology) which is predictive agonist antagonist activity at H3R. Using R-SAT, we screened over 250,000 small molecule compounds for Over 10 non-imidazole chemotypes were identified with...