A5022011608- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- interferon and immune responses
- Nanoplatforms for cancer theranostics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Microfluidic and Capillary Electrophoresis Applications
- DNA Repair Mechanisms
- RNA modifications and cancer
- Nanopore and Nanochannel Transport Studies
- Biosensors and Analytical Detection
- Advanced Nanomaterials in Catalysis
- Adenosine and Purinergic Signaling
- Electrochemical sensors and biosensors
- Photodynamic Therapy Research Studies
- Electrochemical Analysis and Applications
- Genomics and Chromatin Dynamics
- Immune Response and Inflammation
- Molecular Junctions and Nanostructures
- Metal-Organic Frameworks: Synthesis and Applications
- Mathematical and Theoretical Epidemiology and Ecology Models
- Gene Regulatory Network Analysis
- Biochemical and Molecular Research
National Center for Nanoscience and Technology
2022-2025
University of Chinese Academy of Sciences
2018-2025
Chinese Academy of Sciences
2017-2023
Research Center for Eco-Environmental Sciences
2017-2023
Beijing Administration Institute
2020
Xi'an University of Technology
2009
Abstract Amplified ATP imaging in inflammatory cells is highly desirable. However, the spatial selectivity of current amplification methods limited, that is, signal performed systemically and not a disease site‐specific manner. Here we present versatile strategy, termed enzymatically triggerable, aptamer‐based (ETA‐SA), enables cell‐specific through spatially‐resolved amplification. The ETA‐SA leverages translocated enzyme to activate DNA aptamer probes further drive cascade reactions...
Abstract Tracking spatial and temporal dynamics of bioactive molecules such as enzymes responding to therapeutic treatment is highly important for understanding the related functions. However, in situ molecular imaging at subcellular level during photodynamic therapy (PDT) has been hampered by limitations existing methods. Herein, we present a multifunctional nanoplatform (termed UR‐HAPT) that able simultaneously monitor human apurinic/apyrimidinic endonuclease 1 (APE1) near‐infrared (NIR)...
Abstract Despite significant progress in DNA self‐assembly for interfacing with biology, spatiotemporally controlled regulation of biological process via situ dynamic assembly remains an outstanding challenge. Here, we report optically triggered and disassembly strategy that enables on‐demand activation termination the cyclic GMP‐AMP synthase (cGAS)‐stimulator interferon genes (STING) signaling pathway. In design, activatable hairpin is engineered a photocleavable group at defined site to...
Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective ncRNA (e.g., microRNA (miRNA)) mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated hairpins is inhibited by the block toehold-mediated strand displacement reaction can be exclusively activated mitochondria-encoded ribosomal RNA (rRNA)...
Homologous recombination (HR), catalyzed in an evolutionarily conserved manner by active RecA/Rad51 nucleofilaments, maintains genomic integrity and promotes biological evolution diversity. The structures of nucleofilaments provide information critical for the entire HR process. By exploiting a unique capillary electrophoresis-laser-induced fluorescence polarization assay, we have discovered form RecA nucleofilament, stimulated ATP hydrolysis, that contains mainly unbound nucleotide sites....
Abstract Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio‐applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria‐targeted tumor treatment. We first...
Abstract Amplified ATP imaging in inflammatory cells is highly desirable. However, the spatial selectivity of current amplification methods limited, that is, signal performed systemically and not a disease site‐specific manner. Here we present versatile strategy, termed enzymatically triggerable, aptamer‐based (ETA‐SA), enables cell‐specific through spatially‐resolved amplification. The ETA‐SA leverages translocated enzyme to activate DNA aptamer probes further drive cascade reactions...
Benefiting from specific target recognition by antibodies, the immunoassay is one of widely used assays for detection biologically and environmentally important small molecules in broad fields. It can be challenge to isolate their antibody complex an immobilization-free with separation small-molecule targets. Here we present mediated a triply functional DNA probe. A strand dually labeled fluorophore molecule. This probe integrates three functions, including binding antibody, signal reporting...
Abstract Tracking spatial and temporal dynamics of bioactive molecules such as enzymes responding to therapeutic treatment is highly important for understanding the related functions. However, in situ molecular imaging at subcellular level during photodynamic therapy (PDT) has been hampered by limitations existing methods. Herein, we present a multifunctional nanoplatform (termed UR‐HAPT) that able simultaneously monitor human apurinic/apyrimidinic endonuclease 1 (APE1) near‐infrared (NIR)...
Abstract Despite significant progress in DNA self‐assembly for interfacing with biology, spatiotemporally controlled regulation of biological process via situ dynamic assembly remains an outstanding challenge. Here, we report optically triggered and disassembly strategy that enables on‐demand activation termination the cyclic GMP‐AMP synthase (cGAS)‐stimulator interferon genes (STING) signaling pathway. In design, activatable hairpin is engineered a photocleavable group at defined site to...
Comprehensive Summary Sensitive and specific imaging of tumor‐associated biomarkers offers an effective way for cancer diagnosis. However, most the reported probes are limited to targeting a single biomarker, which may impair precision. Here, we report aptamer‐based, AND‐gate DNA nanodevice that relies on sequential processing two proteins produce fluorescent signal precise tumor imaging. The integrates AS1411 aptamer bind cell surface nucleolin sensor respond intracellular...
Undissociated binding of SSB to single-stranded DNA facilitates fast gel-free capillary electrophoresis analysis fundamentally important strand exchange reactions.
Affinity Capillary Electrophoresis (ACE) is a useful analytical tool to study noncovalent interactions. However, it remains challenging for ACE measure weak and unstable interactions due the fast dissociation of binding complex possible destruction by high electric field. In this study, we proposed two-direction migration strategy that enables detect but important decreasing distance controlling opposite direction free probe. By synthesizing polyacrylamide-coated neutral capillary,...
Abstract Despite advances in the controlled reconfiguration of DNA structures for biological applications, dearth strategies that allow orthogonal regulation immune pathways remains a challenge. Here, we report first time an endogenous and exogenous tandem‐regulated assembly strategy enables orthogonally stimulation cyclic GMP‐AMP synthase (cGAS)‐stimulator interferon genes (STING) pathway. A motif containing two palindromic sequences is engineered with abasic site (AP)‐connected blocking...
The recombinase RecA/Rad51 ATPase family proteins catalyze paramount DNA strand exchange reactions that are critically involved in maintaining genome integrity. However, it remains unclear how proceeds when encountering RecA-free defects nucleoprotein filaments. Herein, by designing a series of unique substrates (e.g. truncated or conjugated incoming single-stranded DNA, and extended donor double-stranded DNA) developing two-color alternating excitation-modified single-molecule real-time...
Abstract Despite advances in the controlled reconfiguration of DNA structures for biological applications, dearth strategies that allow orthogonal regulation immune pathways remains a challenge. Here, we report first time an endogenous and exogenous tandem‐regulated assembly strategy enables orthogonally stimulation cyclic GMP‐AMP synthase (cGAS)‐stimulator interferon genes (STING) pathway. A motif containing two palindromic sequences is engineered with abasic site (AP)‐connected blocking...