A5068123146- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Adolescent and Pediatric Healthcare
- Neuroscience of respiration and sleep
- MicroRNA in disease regulation
- Calcium signaling and nucleotide metabolism
- Cancer-related molecular mechanisms research
- Cytomegalovirus and herpesvirus research
- Kawasaki Disease and Coronary Complications
Universität Hamburg
2019-2024
University Medical Center Hamburg-Eppendorf
2019-2024
ABSTRACT Cells release heterogeneous nano‐sized vesicles either as exosomes, being derived from endosomal compartments, or through budding the plasma membrane so‐called microvesicles, commonly referred to extracellular (EVs). EVs are known for their important roles in mammalian physiology and disease pathogenesis provide a potential biomarker source cancer patients. generally often analysed bulk using Western blotting by bead‐based flow‐cytometry or, with limited parameters, nanoparticle...
Abstract Immune cells at sites of inflammation are continuously activated by local antigens and cytokines, regulatory mechanisms must be enacted to control inflammation. The stepwise hydrolysis extracellular ATP ectonucleotidases CD39 CD73 generates adenosine, a potent immune suppressor. Here we report that human effector CD8 T contribute adenosine production releasing CD73-containing vesicles upon activation. These have AMPase activity, the resulting mediates suppression independently...
Abstract Extracellular ATP activates the P2X7 receptor, leading to inflammasome activation and release of pro‐inflammatory cytokines in monocytes. However, a detailed analysis receptor expression function human T cell compartment has not been reported. Here, we used P2X7‐specific nanobody assess membrane on peripheral lymphocyte subsets. The results show that innate‐like cells, which effectively react innate stimuli by secreting high amounts cytokines, have highest compartment. Using Tγδ...
ATP and its metabolites are important extracellular signal transmitters acting on purinergic P2 P1 receptors. Most cells can actively secrete in response to a variety of external stimuli such as gating the P2X7 receptor. We used Yac-1 murine lymphoma study P2X7-mediated release. These co-express ADP-ribosyltransferase ARTC2, permitting by NAD+-dependent ADP-ribosylation without need add exogenous ATP. released into space within minutes after stimulation with NAD+. This was blocked...
The deubiquitinating enzyme ubiquitin C-terminal hydrolase-L1 (UCH-L1) is required for the maintenance of axonal integrity in neurons and thought to regulate intracellular pool brain. In this study, we show that UCH-L1 has an immunological function dendritic cell (DC) Ag cross-presentation. expressed mouse kidney, spleen, bone marrow-derived DCs, its expression activity are regulated by immune stimuli LPS IFN-γ. UCH-L1-deficient mice have significantly reduced ability cross-prime CD8 T cells...