A5075371599- Environmental Toxicology and Ecotoxicology
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Computational Drug Discovery Methods
- Reproductive biology and impacts on aquatic species
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Molecular Biology Techniques and Applications
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Carcinogens and Genotoxicity Assessment
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Physiological and biochemical adaptations
- CRISPR and Genetic Engineering
- Animal testing and alternatives
- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Microbial bioremediation and biosurfactants
- Pesticide and Herbicide Environmental Studies
- Bacteriophages and microbial interactions
- Pesticide Exposure and Toxicity
- Genomics and Chromatin Dynamics
- Environmental DNA in Biodiversity Studies
U.S. Army Engineer Research and Development Center
2015-2024
United States Army
2013-2023
United States Army Corps of Engineers
2004-2019
Defense Threat Reduction Agency
2019
Adelphi Laboratory Center
2019
Environmental Protection Agency
2019
University of Otago
2019
Geotechnical and Structures Laboratory
2015-2018
Virginia Commonwealth University
2015
United States Department of the Army
2009-2013
A quantitative adverse outcome pathway (qAOP) consists of one or more biologically based, computational models describing key event relationships linking a molecular initiating (MIE) to an outcome. qAOP provides quantitative, dose-response, and time-course predictions that can support regulatory decision-making. Herein we describe several facets qAOPs, including (a) motivation for development, (b) technical considerations, (c) evaluation confidence, (d) potential applications. The used as...
Growth of Alcaligenes eutrophus JMP134 on 2,4-dichlorophenoxyacetate requires a 2,4-dichlorphenol hydroxylase encoded by gene tfdB. Catabolism either or 3-chlorobenzoate involves enzymes the chlorocatechol oxidative operon consisting tfdCDEF, which converts 3-chloro- and 3,5-dichlorocatechol to maleylacetate chloromaleylacetate, respectively. Transposon mutagenesis has localized tfdB tfdCDEF EcoRI fragment B plasmid pJP4 (R. H. Don, A. J. Wieghtman, H.-J. Knackmuss, K. N. Timmis, Bacteriol....
ADVERTISEMENT RETURN TO ISSUEPREVVIEWPOINTNEXTToxicogenomics in Regulatory EcotoxicologyGerald T. Ankley, George P. Daston, Sigmund J. Degitz, Nancy D. Denslow, Robert A. Hoke, Sean W. Kennedy, Ann L. Miracle, Edward Perkins, Jason Snape, Donald E. Tillitt, Charles R. Tyler, and VersteegCite this: Environ. Sci. Technol. 2006, 40, 13, 4055–4065Publication Date (Web):July 1, 2006Publication History Published online1 July 2006Published inissue 1...
Toxicogenomics has provided innovative approaches to chemical screening, risk assessment, and predictive toxicology. If applied ecotoxicology, genomics tools could greatly enhance the ability understand modes of toxicity in environmentally relevant organisms. Daphnia magna, a small aquatic crustacean, is considered "keystone" species ecological food webs an indicator for toxicant exposure. Our objective was demonstrate potential utility gene expression profiling ecotoxicology by identifying...
Efforts are underway to transform regulatory toxicology and chemical safety assessment from a largely empirical science based on direct observation of apical toxicity outcomes in whole organism tests predictive one which risk inferred accumulated mechanistic understanding.The adverse outcome pathway (AOP) framework provides systematic approach for organizing knowledge that may support such inference.Likewise, computational models biological systems at various scales provide another means...
Ecotoxicogenomic approaches to environmental monitoring provide holistic information, offer insight into modes of action, and help assess the causal agents potential toxicity effluents beyond traditional end points death reproduction. Recent investigations toxicant exposure indicate dose-dependent changes are a key issue in interpreting genomic studies. Additionally, there is interest developing methods integrate gene expression studies regulation, No Observed Transcriptional Effect Level...
Effects of bisphenol A (BPA) on ovarian transcript profiles as well targeted end points with endocrine/reproductive relevance were examined in two fish species, fathead minnow (Pimephales promelas) and zebrafish (Danio rerio), exposed parallel using matched experimental designs. Four days waterborne exposure to 10 μg BPA/L caused significant vitellogenin induction both species. However, less sensitive effects hepatic gene expression steroid production than the magnitude was more modest...
Nanoparticles are of concern because widespread use, but it is unclear if metal nanoparticles cause effects directly or indirectly. We explored whether polyvinylpyrrolidone-coated silver (PVP-AgNPs) through intact dissolved silver. Females the model species fathead minnow (Pimephales promelas) were exposed to either 4.8 μg/L AgNO3 61.4 PVP-AgNPs for 96h. Microarray analyses used identify impacted receptors and toxicity pathways in liver brain tissues that confirmed using vitro mammalian...
Our ability to conduct whole-organism toxicity tests understand chemical safety has been outpaced by the synthesis of new chemicals for a wide variety commercial applications. As result, scientists and risk assessors are turning mechanistically based studies increase efficiencies in assessment making greater use vitro silico methods evaluate potential environmental human health hazards. In this context, adverse outcome pathway (AOP) framework gained traction regulatory science because it...
ABSTRACT Dredged harbor sediment contaminated with polycyclic aromatic hydrocarbons (PAHs) was removed from the Milwaukee Confined Disposal Facility and examined for in situ biodegradative capacity. Molecular techniques were used to determine successional characteristics of indigenous microbiota during a 4-month bioslurry evaluation. Ester-linked phospholipid fatty acids (PLFA), multiplex PCR targeted genes, radiorespirometry define microbial phenotypic, genotypic, metabolic responses,...
Abstract Background The regulation of gene expression is achieved through regulatory networks (GRNs) in which collections genes interact with one another and other substances a cell. In order to understand the underlying function organisms, it necessary study behavior network context. Several computational approaches are available for modeling different datasets. optimize GRN, these must be compared evaluated terms accuracy efficiency. Results this paper, two important networks,...
Abstract Background Aquatic organisms are continuously exposed to complex mixtures of chemicals, many which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order analyze the effects and mechanisms action estrogen/anti-estrogen mixtures, we male fathead minnows ( Pimephales promelas ) for 48 hours via water 2, 5, 10, 50 ng 17α-ethinylestradiol (EE 2 )/L, 100 ZM 189,154/L (a potent antiestrogen known block activity...
Abstract The toxicological effects of many stressors are mediated through unknown, or incompletely characterized, mechanisms action. application reverse engineering complex interaction networks from high dimensional omics data (gene, protein, metabolic, signaling) can be used to overcome these limitations. This approach was characterize adverse outcome pathways (AOPs) for chemicals that disrupt the hypothalamus‐pituitary‐gonadal endocrine axis in fathead minnows (FHM, Pimephales promelas )....
Permafrost is thawing at unprecedented rates, significantly altering landscapes and ecosystem trajectories by changing subsurface conditions, vegetation characteristics, soil properties. Dormant microbes become active as temperatures rise permafrost soils warm thaw. To determine the effects of sample location warming on microbiome, we collected from five distinct locations within Cold Regions Research Engineering Laboratory's Tunnel (PT) near Fairbanks, Alaska warmed them in a laboratory...
Xenobiotics such as explosives and pesticides released into the environment can have lethal sublethal impacts on soil organisms earthworms with potential subsequent at higher trophic levels. To better understand molecular toxicological mechanisms of 2,4,6-trinitrotoluene (TNT), a commonly used explosive, in Eisenia fetida, were exposed to gradient TNT-spiked soils for 28 days gene expression examined using 4032 cDNA microarray. Reproduction was increased low doses TNT, whereas high TNT...
Reverse engineering of gene regulatory networks using information theory models has received much attention due to its simplicity, low computational cost, and capability inferring large networks. One the major problems with is determine threshold which defines relationships between genes. The minimum description length (MDL) principle been implemented overcome this problem. MDL sum model data encoding length. A user-specified fine tuning parameter used as control mechanism encoding, but it...