- Date Palm Research Studies
- Genetic and Environmental Crop Studies
- Genetic diversity and population structure
- Banana Cultivation and Research
- Botanical Research and Chemistry
- Nuts composition and effects
- Oil Palm Production and Sustainability
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Identification and Quantification in Food
- Horticultural and Viticultural Research
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Allergic Rhinitis and Sensitization
- Plant Reproductive Biology
- Phytoplasmas and Hemiptera pathogens
- Plant-Microbe Interactions and Immunity
- Leaf Properties and Growth Measurement
- Plant and animal studies
- Agricultural pest management studies
- Agricultural Research and Practices
- Seed and Plant Biochemistry
- Chromium effects and bioremediation
- Plant Growth Enhancement Techniques
- Microbial Community Ecology and Physiology
Tunis El Manar University
2012-2024
Tunis University
2007-2024
Laboratoire de Microbiologie et Génétique Moléculaires
2013-2015
Whether sex chromosomes are differentiated is an important aspect of our knowledge dioecious plants, such as date palm (Phoenix dactylifera). In this crop plant, the female individuals produce dates, and thus more valuable sex. However, there no way to identify plants before reproductive age, sex-determining mechanism still unclear. To sex-linked microsatellite markers, we surveyed a set 52 male 55 genotypes representing geographical diversity species. We found three genetically linked loci...
Date palms (Phoenix dactylifera, Arecaceae) are of great economic and ecological value to the oasis agriculture arid semi-arid areas. However, despite availability a large date palm germplasm spreading from Atlantic shores Southern Asia, improvement species is being hampered by lack information on global genetic diversity population structure. In order contribute varietal provide new insights influence geographic origins human activity structure palm, this study analysed species.Genetic...
Fourteen microsatellite loci of Phoenix dactylifera were targeted to examine the genetic diversity in Tunisian date-palms germplasm. They showed a high level polymorphism 49 accessions from three main oases with little geographic structure within Tunisia. The data agrees previous analyses germplasm using other molecular markers. 100% local successfully fingerprinted and easily distinguished by help only loci. possibility microsatellites for large scale labelling offshoots vitro plantlets...
The biogeography of the date palm (Phoenix dactylifera L.), origin, structure and dynamics its agrobiodiversity, relationships to other species Phoenix are reviewed. is confirmed as a distinct has closest affinities with P. sylvestris atlantica. Multiple origins domestication likely, within two highly primary gene pools, one oriental (Middle East) occidental (south-western Europe north-western Africa), subsequently partially admixed, especially in intermediate zone North Africa. A rationale...
Inter Simple Sequence Repeat (ISSR) markers involve polymerase chain reaction (PCR) amplification of DNA using a single primer composed microsatellite sequence. ISSR technology rapidly reveals high polymorphic fingerprints and determines genetic diversity. We used generated from selected primers toassess diversity among set Tunisian date palm varieties. Seven were to cluster 12 varieties 77 sufficient identify all the These discrete molecular estimate distances accessions examine their...
Abstract Crop diversity is shaped by biological and social processes interacting at different spatiotemporal scales. Here, we combined population genetics ethnobotany to investigate date palm ( Phoenix dactylifera L.) in Siwa Oasis, Egypt. Based on interviews with farmers observation of practices the field, collected 149 palms from Oasis 27 uncultivated abandoned oases surrounding desert. Using genotyping data 18 nuclear plastid microsatellite loci, confirmed that some named types each...
Understanding the driving forces and molecular processes underlying dioecy sex chromosome evolution, leading from hermaphroditism to occurrence of male female individuals, is considerable interest in fundamental applied research. The genus Phoenix, belonging Arecaceae family, consists uniquely dioecious species. Phylogenetic data suggest that Phoenix has diverged a hermaphroditic ancestor which also shared with its closest relatives. We have investigated cessation recombination...
The genetic diversity organization and evaluation of relationships within Tunisian date-palm cultivars were assessed using the random amplified microsatellite polymorphism (RAMPO) fragment length (AFLP) methods. 18 combinations random/ISSR primers six AFLP tested with DNAs isolated from 40 cultivars. Our results show that both markers systems, germplasm is characterized by a large continuous diversity. In addition, due to greater number per assay, technique seems be more informative than...
SummaryAmplified fragment length polymorphisms (AFLP) were used successfully to survey genetic diversity in 40 ecotypes of date-palm (Phoenix dactylifera L.) collected from oases Tunisia. Six primer pairs screened assess their ability detect polymorphism this tree crop. As a result, total 428 AFLPs have been generated and estimate distances which ranged 0.07 – 0.63. A large, typically continuous, range characterises Tunisian germplasm. In addition, the UPGMA dendrogram derived these data...
The endemic palm from the Canary Islands, Phoenix canariensis, is one of most distinctive elements Canarian vegetation landscape, contributing to cultural, economic and environmental aspects. One main conservation problems facing this iconic anthropogenic hybridization with other species, particularly dactylifera, which has been introduced extensively throughout its geographical range. Therefore, it important obtain a genetic tool that addresses different issues may have an impact on...
The Cape Verde palm tree,
Date palm (Phœnix dactylifera L.) like other crop species in the arid Mediterranean region is being threatened by genetic erosion and climate change. Therefore, understanding assessment of diversity extent this a primary requisite for preserving these resources. This study was designed to quantify potential Tunisian male date palms using set agro-morphological characteristics i.e. flowering traits, inflorescence morphology pollen quality. The time traits exhibited trend precocious phenotype...
Mauritanian date palm cultivars and progenies of two controlled crosses were analyzed according to the identity mitochondrial plasmid-like DNAs. Starting from total genomic DNA appropriate primers, polymerase chain reaction was designed amplify either a 373-bp or 265-bp fragments corresponding S R-plasmid respectively. Data proved that 5 out 10 studied have exhibited suggesting their resistance fusariosis. The existence intra-cultivar variability has also been revealed in cv. Ahmar. In...
Citrus are one of the most cultivated crops in world. Economically, they very important fruit trees Tunisia. Little is known about genetic diversity Tunisian germplasm. Exploring this a prerequisite for identification and characterization local germplasm to circumvent controlling erosion caused by biotic abiotic stress aid its conservation use. In present study, we explored 20 orange cultivars [Citrus sinensis (L.) Osbeck] established their relationships using seven simple sequence repeat...
Heavy metal pollution is a major environmental issue that has negative impact on soil quality and food security. As result, heavy removal or remediation from hazardous sites become mandatory. Bioremediation based microorganisms promising method to remediate metal-contaminated areas due its eco-friendly, cost-effective, highly efficient characteristics. This study aimed isolate, identify characterize rhizospheric bacteria able resist, reduce, detoxify metals (chromium [Cr], nickel [Ni],...
DNA variation
Knowledge of the level storage effect on viability, germination capacity and bioactive components date palm pollen is important to identify best conditions that can maintain its agronomic medicinal properties for long-term preservation. In this context, present work aimed evaluate reproductive quality total polyphenol content pollen. Pollen 24 male genotypes was collected 3 successive years from 2017 2019 stored at +4 °C. Alexander staining in vitro were used estimate quality. The...
ABSTRACTMost male date palms available for pollinating different female cultivars have mainly originated from seed propagation resulting in many local males that represent a source of genetic diversity. Favourable fruit production is related to the quality pollen and its compatibility with certain variety. Therefore, characterisation progenitors should be first step establish selection programme produce superior through procedures. In this study, diversity population structure 72 palm...