A5031922746- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Antioxidant Activity and Oxidative Stress
- Genomics, phytochemicals, and oxidative stress
- Photosynthetic Processes and Mechanisms
- Adenosine and Purinergic Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- melanin and skin pigmentation
- Metal-Organic Frameworks: Synthesis and Applications
- SARS-CoV-2 and COVID-19 Research
- HER2/EGFR in Cancer Research
- Free Radicals and Antioxidants
- Immunotherapy and Immune Responses
- Quantum-Dot Cellular Automata
- DNA and Nucleic Acid Chemistry
- Algal biology and biofuel production
- Biochemical Acid Research Studies
- Curcumin's Biomedical Applications
- Glycosylation and Glycoproteins Research
- Boron Compounds in Chemistry
- Cutaneous Melanoma Detection and Management
Institute of Gene Biology
2013-2025
Russian Academy of Sciences
2012-2019
Laboratory of Molecular Genetics
2017
Lomonosov Moscow State University
2010-2014
Gamma-ray emitting 111In, which is extensively used for imaging, also a source of short-range Auger electrons (AE). While exhibiting negligible effect outside cells, these AE become highly toxic near DNA within the cell nucleus. Therefore, radionuclides can be as therapeutic anticancer agent if delivered precisely into nuclei tumor target cells. Modular nanotransporters (MNTs) designed to provide receptor-targeted delivery cargoes cells are perspective candidates specific intracellular...
This study evaluates the potential utility of a modular nanotransporter (MNT) for enhancing nuclear delivery and cytotoxicity Auger electron emitter 125I in cancer cells that overexpress epidermal growth factor receptor (EGFR).MNTs are recombinant multifunctional polypeptides we have developed achieving selective short-range therapeutics into cells. MNTs contain functional modules binding, internalization, endosomal escape translocation, thereby facilitating transport drugs from cell surface...
To compare the effectiveness of various bioactive agents reversibly acting within a cell on target intracellular macromolecule, it is necessary to assess effective cytoplasmic concentrations delivered agents. In this work, based simple equilibrium model and cellular thermal shift assay (CETSA), an approach proposed both agent protein. This was tested by evaluating average nuclear factor erythroid 2-related 2 (Nrf2) Kelch-like ECH-associated-protein 1 (Keap1) proteins in cytoplasm for five...
Brachytherapy, or internal radiation therapy, is a highly effective treatment option for localized tumors. Herein, injectable and biodegradable metal-organic frameworks (MOFs) were engineered to deliver the therapeutic radioisotope yttrium-90 (90Y). Particles of bimetallic MIL-100(Fe,Y) Y-BTC, doped with 90Y 88Y, synthesized in single step retained radioyttrium various buffer solutions. Tumor injectability retention evaluated using tumor-bearing mice. In vivo analysis calculations showed...
Purpose: Modular nanotransporters (MNTs) are artificial multifunctional systems designed to facilitate receptor-specific transport from the cell surface into nucleus through inclusion of polypeptide domains for accomplishing receptor binding and internalization, as well sequential endosomal escape nuclear translocation. The objective this study was develop a new MNT targeted at folate receptors (FRs) precise delivery therapeutic cargo nuclei FR-positive cells evaluate its potential,...
Comparative study of spectroscopic features reveals the structural determinants keto-carotenoids in photoactive water-soluble proteins.
Purpose: Modular nanotransporters (MNTs) are a polyfunctional platform designed to achieve receptor-specific delivery of short-range therapeutics into the cell nucleus by receptor-mediated endocytosis, endosome escape, and targeted nuclear transport. This study evaluated potential utility MNT in tandem with Auger electron emitting 111 In for cancer therapy. Methods: Three MNTs developed target either melanocortin receptor-1 (MC1R), folate receptor (FR), or epidermal growth factor (EGFR) that...
Since cell nucleus is one of the most vulnerable compartments, maximum therapeutic effect from a variety locally acting agents, such as photosensitizers, alfa-emitters, Auger electron emitters, will be expected when they get there. Therefore, targeted delivery these agents into nuclei target tumor cells necessary for their anticancer effects and minimization side effects. Modular nanotransporters (MNT) are artificial polypeptides comprising several predefined modules that recognize cell,...
To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems far from being efficient. Here we demonstrate that recently discovered nanometer-sized water-soluble carotenoprotein Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract via carotenoid-mediated homodimerization, yielding...
The study of oxidative stress in cells and ways to prevent it attract increasing attention. Antioxidant defense can be activated by releasing the transcription factor Nrf2 from a complex with Keap1, its inhibitor protein. aim work was effect modular nanotransporter (MNT) carrying an R1 anti-Keap1 monobody (MNT
Treatment of various diseases, in particular cancer, usually needs targeting biologically active molecules at a selected subcellular compartment. We modified our previously developed modular nanotransporters (MNTs) for mitochondria. The new MNTs are capable binding to the protein predominantly localized on outer mitochondrial membrane, Keap1. These possessing antiKeap1 monobody co-localize with mitochondria upon addition cells. They efficiently interact Keap1 both solution and within living...
Treatment of various diseases, in particular cancer, usually requires the targeting biologically active molecules at a selected subcellular compartment. We modified our previously developed modular nanotransporters (MNTs) for mitochondria. The new MNTs are capable binding to protein predominantly localized on outer mitochondrial membrane, Keap1. These possessing antiKeap1 monobody co-localize with mitochondria upon addition cells. They efficiently interact Keap1 both solution and within...
Abstract Brachytherapy, or intratumoral radiation therapy, is a highly effective treatment option for localized tumors. Herein, we engineered injectable and biodegradable metal-organic frameworks (MOFs) to deliver the therapeutic radioisotope yttrium-90 ( 90 Y). Particles of bimetallic MIL-100(Fe,Y) Y-BTC, doped with Y 88 Y, were synthesized in single step retained radioyttrium various buffer solutions. Tumor injectability retention evaluated using tumor-bearing mice. In vivo analysis...