A5051563559- Multiple Myeloma Research and Treatments
- DNA Repair Mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Carcinogens and Genotoxicity Assessment
- Effects of Radiation Exposure
- Cancer-related Molecular Pathways
- Blood groups and transfusion
- Acute Myeloid Leukemia Research
- Platelet Disorders and Treatments
- Peptidase Inhibition and Analysis
- Radiation Therapy and Dosimetry
- Complement system in diseases
- Drug Transport and Resistance Mechanisms
- Immunodeficiency and Autoimmune Disorders
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Renal Diseases and Glomerulopathies
- Acute Lymphoblastic Leukemia research
- Radiation Effects and Dosimetry
- PARP inhibition in cancer therapy
- Chronic Myeloid Leukemia Treatments
- Advanced Proteomics Techniques and Applications
Mayo Clinic in Arizona
2016-2025
Mayo Clinic
2016-2025
WinnMed
2014-2025
University of Alberta
2015-2024
University of Minnesota Rochester
2019-2024
Royal Adelaide Hospital
2024
Cook Medical (Ireland)
2020-2022
Southwest Washington Medical Center
2021-2022
Mayo Clinic in Florida
2012-2020
Emory University Hospital
2006-2020
To determine if repeated intravenous exposures to gadolinium-based contrast agents (GBCAs) are associated with neuronal tissue deposition.In this institutional review board-approved single-center study, signal intensities from T1-weighted magnetic resonance (MR) images and postmortem samples 13 patients who underwent at least four GBCA-enhanced brain MR examinations between 2000 2014 (contrast group) were compared those 10 did not receive GBCA (control group). Antemortem consent was obtained...
Insulin resistance is a common problem associated with infections and cancer and, most importantly, the central component of non-insulin-dependent diabetes mellitus. We have recently shown that tumor necrosis factor (TNF) alpha key mediator insulin in animal models Here, we investigate how TNF-alpha interferes action. Chronic exposure adipocytes to low concentrations strongly inhibits insulin-stimulated glucose uptake. Concurrently, treatment causes moderate decrease autophosphorylation...
Purpose To determine whether gadolinium deposits in neural tissues of patients with intracranial abnormalities following intravenous gadolinium-based contrast agent (GBCA) exposure might be related to blood-brain barrier integrity by studying adult normal brain pathologic characteristics. Materials and Methods After obtaining antemortem consent institutional review board approval, the authors compared postmortem neuronal tissue samples from five who had undergone four 18 gadolinium-enhanced...
Purpose To compare gadolinium tissue concentrations of multiple linear and macrocyclic chelates in a rat model to better understand the scope extent deposition following intravenous doses gadolinium-based contrast agent (GBCA). Materials Methods In this Institutional Animal Care Use Committee-approved study, healthy rats received 20 injections 2.5 mmol per kilogram (gadolinium-exposed group) or saline (control over 26-day period. Unenhanced T1 signal intensities dentate nucleus were measured...
Tumors and tumor-derived cell lines contain polyploid giant cells with significantly elevated genomic content, often multiple nuclei. The frequency of can increase markedly following anticancer treatment. Although enter a dormant phase therefore do not form macroscopic colonies (aggregates ≥50 cells) in the conventional vitro colony formation assay, they remain viable metabolically active. purpose this commentary is to underscore potential importance polyploid/multinucleated metastasis...
Abstract Plasma cell disorders (PCDs) are identified in the clinical lab by detecting monoclonal immunoglobulin (M-protein) which they produce. Traditionally, serum protein electrophoresis methods have been utilized to detect and isotype M-proteins. Increasing demands low-level disease new therapeutic treatments stretched electrophoretic their analytical limits. Newer techniques based on mass spectrometry (MS) emerging improved performance. MS is gaining traction into laboratories, has...
A monoclonal gammopathy is defined by the detection a immunoglobulin (M-protein). In clinical practice, M-protein detected protein gel electrophoresis (PEL) and immunofixation (IFE). We theorized that molecular mass could be used instead of electrophoretic patterns to identify quantify because each light heavy chain has unique amino acid sequence thus whose increased concentration distinguished from normal polyclonal background. addition, we surmised top-down MS isotype constant region with...
Electrophoretic separation of serum and urine proteins has played a central role in diagnosing monitoring plasma cell disorders. Despite limitations resolution analytical sensitivity, plus the necessity for adjunct methods, protein gel electrophoresis immunofixation (IFE) remain front-line tests.We developed MALDI mass spectrometry-based assay that was simple to perform, automatable, analytically sensitive, applicable analyzing wide variety monoclonal (M-proteins) encountered clinically....
The detection and quantification of monoclonal-proteins (M-proteins) are necessary for the diagnosis evaluation response in plasma cell dyscrasias. Immunoglobulin enrichment-coupled with matrix-assisted laser desorption ionization time-of-flight mass-spectrometry (MASS-FIX) is a simple inexpensive method to identify M-proteins, but its clinical generalizability has not yet been elucidated. We compared MASS-FIX protein electrophoresis (PEL), serum/urine immunofixation-electrophoresis (IFE),...
This study determines the extent of deposition in gadolinium-based contrast agent–exposed pediatric population and whether prior observations were related to age-dependent breakdown blood blood-brain barrier.
Abstract Objective: To explore the possible relationship between single nucleotide polymorphisms (SNP) in candidate genes encoding DNA damage recognition/repair/response and steroid metabolism proteins with respect to clinical radiation toxicity a retrospective cohort of patients previously treated three-dimensional conformal radiotherapy (3-DCRT) for prostate cancer. Experimental Design: One hundred twenty-four cancer underwent 3-DCRT at our institution September 1996 December 2000. Of...
We assessed the ability of a mass spectrometry-based technique, called monoclonal immunoglobulin rapid accurate measurement (miRAMM), to extend analytical range M-protein detection in serum samples obtained from myeloma patients stringent complete response (sCR) post-autologous stem cell transplant (ASCT). To aid post ASCT, molecular light chain at diagnosis was determined all (N=30) and used positively identify clones sCR serum. Day 100 post-ASCT, had miRAMM identifiable M-proteins 81%...
Abstract High-sensitivity mass spectrometry assays are available to detect monoclonal immunoglobulins. To better assess the prevalence of gammopathy undetermined significance (MGUS), we identified 300 patients diagnosed with MGUS or related who had a prior negative work-up for proteins as part Olmsted County screening study. Two spectrometry-based detection methods (matrix-assisted laser desorption/ionization-time flight (MALDI-TOF) and immunoglobulin rapid accurate measurements (miRAMM)...