A5005619450- Advanced Fluorescence Microscopy Techniques
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticles: synthesis and applications
- Occupational exposure and asthma
- Luminescence and Fluorescent Materials
- Contact Dermatitis and Allergies
- Glycosylation and Glycoproteins Research
BASF (Germany)
2024
Max Planck Institute for Medical Research
2021-2023
Photoswitchable fluorophores─proteins and synthetic dyes─whose emission is reversibly switched on off upon illumination, are powerful probes for bioimaging, protein tracking, super-resolution microscopy. Compared to proteins, dyes smaller brighter, but their photostability the number of achievable switching cycles in aqueous solutions lower. Inspired by robust photoswitching system natural we designed a supramolecular based fluorescent diarylethene (DAE) cucurbit[7]uril (CB7) (denoted as...
Significance In superresolution fluorescence microscopy, employing synthetic dyes that can be reversibly photoswitched between a nonfluorescent (“dark”) and fluorescent (“bright”) state has been an attractive alternative to using photoswitchable proteins. However, such elusive because they have defied reliable attachment proteins required UV light for photoswitching. Here we prepared “turn-on mode” diarylethenes (fDAEs) are switchable with visible rather than blink bright dark in aqueous...
Here we describe highly compact, click compatible, and photoactivatable dyes for super-resolution fluorescence microscopy (nanoscopy). By combining the xanthone (PaX) core with a tetrazine group, achieve minimally sized sensitive molecular dyads selective labeling of unnatural amino acids introduced by genetic code expansion. We exploit excited state quenching properties group to attenuate photoactivation rates PaX, further reduce overall emission photogenerated fluorophore, providing two...
New photoactivatable fluorescent dyes (rhodamine, carbo- and silicon-rhodamines [SiR]) with emission ranging from green to far red have been prepared, their photophysical properties studied. The photocleavable 2-nitrobenzyloxycarbonyl unit an alpha-carboxyl group as a branching point additional functionality was attached polycyclic lipophilic dye. probes having the HaloTagTM amine (O2) ligand bound dye core were obtained applied for live-cell staining in stable cell lines incorporating...
Since 2020, the REACh regulation requires toxicological data on nanoforms of materials, including assessment their skin-sensitizing properties. Small molecules’ skin sensitization potential can be assessed by new approach methodologies (NAMs) addressing three key events (KE: protein interaction, activation dendritic cells, and keratinocytes) combined in a defined (DA) described OECD guideline 497. In present study, applicability NAMs (DPRA, LuSens, h-CLAT) to nine materials (eight inorganic...
A bright and photostable fluorescent dye with a disulfide (S-S) linker maleimide group (Rho594-S2-mal), as cleavable reactive sites, was synthesized conjugated anti-GFP nanobodies (NB). The binding of EGFP (FRET donor) NB labeled one or two Rho594-S2-mal residues studied in vitro cellulo. cleaved dithiothreitol recovering the donor (FP) signal. bioconjugates (FP-NB-dye) were applied FRET-FLIM assays, confocal imaging, superresolution STED microscopy.
ABSTRACT Here we describe highly compact, click compatible, and photoactivatable dyes for super-resolution fluorescence microscopy (nanoscopy). By combining the xanthone (PaX) core with a tetrazine group, achieve minimally sized sensitive molecular dyads selective labeling of unnatural amino acids introduced by genetic code expansion. We exploit excited state quenching properties group to attenuate photoactivation rates PaX, further reduce overall emission photogenerated fluorophore,...
Abstract New photoactivatable fluorescent dyes (rhodamine, carbo‐ and silicon‐rhodamines [SiR]) with emission ranging from green to far red have been prepared, their photophysical properties studied. The photocleavable 2‐nitrobenzyloxycarbonyl unit an alpha‐carboxyl group as a branching point additional functionality was attached polycyclic lipophilic dye. probes having the HaloTagTM amine (O2) ligand bound dye core were obtained applied for live‐cell staining in stable cell lines...