Biodiversity assessment of marine hard-bottom communities is hindered by the high diversity and size-ranges organisms present. We developed a DNA metabarcoding protocol for biodiversity characterization structurally complex natural communities. used two molecular markers: “Leray fragment” mitochondrial cytochrome c oxidase (COI), which novel primer set was developed, V7 region nuclear small subunit ribosomal RNA (18S). Eight different shallow littoral from National Parks in Spain (one...

The recent blooming of metabarcoding applications to biodiversity studies comes with some relevant methodological debates. One such issue concerns the treatment reads by denoising or clustering methods, which have been wrongly presented as alternatives. It has also suggested that denoised sequence variants should replace clusters basic unit analyses, missing fact are a proxy for species-level entities, in studies. We argue here methods developed and tested ribosomal markers uncritically...

Abstract Metabarcoding is by now a well‐established method for biodiversity assessment in terrestrial, freshwater, and marine environments. data sets are usually used α‐ β‐diversity estimates, that is, interspecies (or inter‐ MOTU [molecular operational taxonomic unit] ) patterns. However, the use of hypervariable metabarcoding markers may provide an enormous amount intraspecies (intra‐ information—mostly untapped so far. The cytochrome oxidase ( COI amplicons gaining momentum studies...

In the marine realm, biomonitoring using environmental DNA (eDNA) of benthic communities requires destructive direct sampling or setting-up settlement structures. Comparatively much less effort is required to sample water column, which can be accessed remotely. this study we assess feasibility obtaining information from eukaryotic by adjacent layer. We studied two different rocky-substrate with a technique based on quadrat sampling. also took replicate samples at four distances (0, 0.5, 1.5,...

The population structure of the edible Atlanto-Mediterranean sea urchin Paracentrotus lividus is described by analysing sequence variation in a fragment mitochondrial gene cytochrome c oxidase subunit I 127 individuals from 12 localities across south-west Europe. study revealed high levels genetic diversity but low structure, suggesting large degree flow between populations and panmixis within each, Mediterranean Atlantic basins. However, we found significant differentiation two basins...

MEPS Marine Ecology Progress Series Contact the journal Facebook (Twitter) RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 122:193-204 (1995) - doi:10.3354/meps122193 Growth and population structure of Paracentrotus lividus (Echinodermata: Echinoidea) in two contrasting habitats Turon X, Giribet G, López S, Palacín C Two populations (Lamarck) from Northwestern Mediterranean were compared ascertain plasticity adaptive value...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 122:179-191 (1995) - doi:10.3354/meps122179 Biological cycles and recruitment of Paracentrotus lividus (Echinodermata: Echinoidea) in two contrasting habitats Lozano J, Galera López S, Turon X, Palacín C, Two populations (Lamarck) from Northwestern Mediterranean were compared ascertain plasticity adaptive...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 441:117-128 (2011) - DOI: https://doi.org/10.3354/meps09359 A wolf in sheep's clothing: carnivory dominant sea urchins Mediterranean Owen S. Wangensteen1,*, Xavier Turon2, Alex García-Cisneros1, Mireia Recasens1, Javier Romero3, Creu Palacín1 1Department of Animal Biology, University Barcelona, 08028 Spain...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 172:239-251 (1998) - doi:10.3354/meps172239 Larval abundance, recruitment and early mortality in Paracentrotus lividus (Echinoidea). Interannual variability plankton-benthos coupling Salvador López1, Xavier Turon1,*, Elena Montero1, Cruz Palacín1, Carlos M. Duarte2, Isabel Tarjuelo1 1Dept of Animal Biology...

We present the global phylogeography of black sea urchin Arbacia lixula, an amphi-Atlantic echinoid with potential to strongly impact shallow rocky ecosystems. Sequences mitochondrial cytochrome c oxidase gene 604 specimens from 24 localities were obtained, covering most distribution area species, including Mediterranean and both shores Atlantic. Genetic diversity measures, phylogeographic patterns, demographic parameters population differentiation analysed. found high haplotype but...

Abstract Aim The Atlanto‐Mediterranean edible purple sea urchin, Paracentrotus lividus , is a commercially exploited keystone species in benthic communities. Its browsing activity can deeply modify the littoral landscape, and changes its abundance are of major conservation concern. This facing nowadays contrasting anthropogenic pressures linked to predator release, exploitation warming. Management this key requires knowledge genetic structure, connectivity local adaptation. Our goal was...

Abstract Aim It has been predicted that there should be concordance between biogeographical and phylogeographical processes structuring multi‐species regional assemblages. We hypothesise oceanographic barriers in the marine environment affect concomitantly distribution connectivity of biota, thus producing congruent structures. also predict macro‐ meio‐eukaryotes will differentially affected by hydrological features. Location The Atlanto‐Mediterranean transition along E Iberian coast marked...

Temporal variability was studied in the common sea urchin Paracentrotus lividus through analysis of genetic composition three yearly cohorts sampled over two consecutive springs a locality northwestern Mediterranean. Individuals were aged using growth ring patterns observed tests and samples genotyped for five microsatellite loci. No reduction diversity relative to sample adult population from same location or within across years. F(ST )and amova results indicated that differentiation...

Abstract Understanding the phylogeography and genetic structure of populations processes responsible patterns therein is crucial for evaluating vulnerability marine species developing management strategies. In this study, we explore how past climatic events ongoing oceanographic demographic have shaped diversity Atlanto-Mediterranean red starfish Echinaster sepositus . The relatively abundant in some areas Mediterranean Sea, but dramatically decreased over recent years due to direct...

Abstract Metabarcoding is by now a well-established method for biodiversity assessment in terrestrial, freshwater and marine environments. datasets are usually used α- β-diversity estimates, that is, interspecies (or inter-MOTU) patterns. However, the use of hypervariable metabarcoding markers may provide an enormous amount intraspecies (intra-MOTU) information - mostly untapped so far. The cytochrome oxidase (COI) amplicons gaining momentum studies targeting eukaryote richness. COI has been...

Spatio-temporal changes in genetic structure among populations provide crucial information on the dynamics of secondary spread for introduced marine species. However, temporal components have rarely been taken into consideration when studying population genetics non-indigenous This study analysed Styela plicata, a solitary ascidian harbours and marinas tropical temperate waters, across spatial scales. A fragment mitochondrial gene Cytochrome Oxidase subunit I (COI) was sequenced from 395...

The taxonomy of the genus Pycnoclavella on European shores has been controversial. Variability in colour patterns and other characters incompleteness descriptions have contributed to confusion. To clarify taxonomic positions variety forms attributable this genus, western Mediterranean north-eastern Atlantic sites were sampled. present study employed morphological a molecular approach, using fragment cytochrome c oxidase I gene 120 colonies for phylogenetic analyses. results reveal an...