A5021342633- Neuroscience and Neuropharmacology Research
- Vestibular and auditory disorders
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Cellular transport and secretion
- Autism Spectrum Disorder Research
- RNA regulation and disease
- Neuroscience of respiration and sleep
- Neurogenesis and neuroplasticity mechanisms
- Advanced Memory and Neural Computing
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic Neurodegenerative Diseases
Institut des Neurosciences Cellulaires et Intégratives
2016-2022
Université de Strasbourg
2017-2022
Centre National de la Recherche Scientifique
2016-2022
Okinawa Institute of Science and Technology Graduate University
2020-2022
Aging weakens memory functions. Exposing healthy rodents or pathological rodent models to environmental enrichment (EE) housing improves their cognitive functions by changing neuronal levels of excitation, cellular signaling, and plasticity, notably in the hippocampus. At molecular level, brain derived-neurotrophic factor (BDNF) represents an important player that supports EE-associated changes. EE facilitation learning was also shown correlate with chromatin acetylation It is not known,...
The segregation of the readily releasable pool synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, frequency-dependent mobilization these is poorly understood. Using slice recordings and modeling activity at cerebellar granule cell to Purkinje synapses mice, we describe two RRP can be recruited upon ultrafast changes stimulation frequency. We show low-frequency stimulations, a first sub-pool gradually silenced, leading...
Information processing by cerebellar molecular layer interneurons (MLIs) plays a crucial role in motor behavior. MLI recruitment is tightly controlled the profile of short-term plasticity (STP) at granule cell (GC)-MLI synapses. While GCs are most numerous neurons brain, STP diversity GC-MLI synapses poorly documented. Here, we studied how single MLIs recruited their distinct GC inputs during burst firing. Using slice recordings individual mice, revealed four classes connections segregated...
Cerebellar granule cells (GCs) make up the majority of all neurons in vertebrate brain, but heterogeneities among GCs and potential functional consequences are poorly understood. Here, we identified unexpected gradients biophysical properties mice. closer to white matter (inner-zone GCs) had higher firing thresholds could sustain with larger current inputs than Purkinje cell layer (outer-zone GCs). Dynamic Clamp experiments showed that inner- outer-zone preferentially respond high-...
The cerebellum drives motor coordination and sequencing of actions at the millisecond timescale through adaptive control cerebellar nuclear output. Cerebellar nuclei integrate high-frequency information from both cortex two main excitatory inputs cerebellum: mossy fibers climbing fiber collaterals. However, how cells process rate timing carried by these is still debated. Here, we investigate influence cortical output, Purkinje cells, on identified neurons in vivo male mice. Using transgenic...
Abstract The ability of the cerebellar cortex to learn from experience ensures accuracy movements and reflex adaptation, processes which require long-term plasticity at granule cell (GC) Purkinje neuron (PN) excitatory synapses. PNs also receive GABAergic inhibitory inputs via GCs activation interneurons; despite involvement inhibition in motor learning, its role is poorly characterized. Here we reveal a functional coupling between ionotropic GABA A receptors low threshold Ca V 3 calcium...
Adeno-associated viral (AAV) vectors, used as vehicles for gene transfer into the brain, are a versatile and powerful tool of modern neuroscience that allow identifying specific neuronal populations, monitoring modulating their activity. For consistent reproducible results, AAV vectors must be engineered so they reliably accurately target cell populations. Furthermore, transgene expression adjusted to sufficient safe levels compatible with physiology studied cells. We undertook effort...
The cerebellar cortex encodes sensorimotor adaptation during skilled locomotor behaviors, however the precise relationship between synaptic connectivity and behavior is unclear. We studied granule cells (GCs) Purkinje (PCs) in murine acute slices using photostimulation of caged glutamate combined with patch-clamp developing or after mice adapted to different contexts. By translating individual maps into graph network entities, we found that juvenile animals undergo critical period...
Abstract Membrane voltage oscillations in layer 1 (L1) of primary sensory cortices might be important indicators cortical gain control, attentional focusing, and signal integration. However, electric field recordings are hampered by the low seal resistance electrodes close to brain surface. To study L1 membrane oscillations, we synthesized a new voltage-sensitive dye, di1-ANNINE (anellated hemicyanine)-6plus, that can diffuse into tissue. We applied it with surgery, leaving dura intact but...
Voltage imaging with cellular resolution in mammalian brain slices is still a challenging task. Here, we describe and validate method for delivery of the voltage-sensitive dye ANNINE-6plus (A6+) into tissue voltage that results higher signal-to-noise ratio (SNR) than conventional bath application methods. The not fully dissolved was injected inferior olive (IO) 0, 1, or 7 days prior to acute slice preparation using stereotactic surgery. We find improves after an extended incubation period...
Abstract In the cerebellum, molecular layer interneurons (MLIs) play an essential role in motor behavior by exerting precise temporal control of Purkinje cells, sole output cerebellar cortex. The recruitment MLIs is tightly controlled release glutamate from granule cells (GCs) during high-frequency activities. Here we study how single are recruited their distinct unitary GC inputs burst stimulations. Stimulation individual GC-MLI synapses revealed four classes connections segregated profile...
Abstract From planification to execution, cerebellar microcircuits encode different features of skilled movements. However, it is unknown whether synaptic connectivity maps movement in a motor context specific manner. Here we investigated the spatial organization excitatory mice cortex locomotor contexts: during development and normal, trained or altered conditions. We combined optical, electrophysiological graph modelling approaches describe between granule cells (GCs) Purkinje (PCs)....
ABSTRACT The segregation of the readily releasable pool synaptic vesicles (RRP) in sub-pool which are differentially poised for exocytosis shapes short-term plasticity at depressing synapses. Here, we used vitro recording and modeling activity facilitating mice cerebellar granule cell to Purkinje synapse demonstrate existence two sub-pools RRP that can be recruited upon fast changes stimulation frequency. We show low-frequency stimulation, a population fully-releasable is silenced, leading...
Abstract Cerebellar granule cells (GCs) making up majority of all the neurons in vertebrate brain, but heterogeneities among GCs and potential functional consequences are poorly understood. Here, we identified unexpected gradients biophysical properties GCs. closer to white matter (inner-zone GCs) had higher firing thresholds could sustain with larger current inputs. Dynamic clamp experiments showed that inner- outer-zone preferentially respond high- low-frequency mossy fiber inputs,...