A5064428230- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Molecular Biology Techniques and Applications
- X-ray Diffraction in Crystallography
- Radiopharmaceutical Chemistry and Applications
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Antimicrobial Peptides and Activities
- Synthesis and Biological Evaluation
- Synthesis and Reactivity of Heterocycles
- Fluorine in Organic Chemistry
- DNA and Nucleic Acid Chemistry
- Microbial Natural Products and Biosynthesis
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
Helmholtz Centre for Infection Research
2016-2022
Pseudomonas aeruginosa is a major cause of nosocomial infections and also leads to severe exacerbations in cystic fibrosis or chronic obstructive pulmonary disease. Three intertwined quorum sensing systems control virulence P. aeruginosa, with the rhl circuit playing leading role late infections. The majority traits controlled by transcription factor RhlR depend on PqsE, dispensable thioesterase Quinolone Signal (PQS) biosynthesis that interferes through an enigmatic mechanism likely...
Pseudomonas aeruginosa uses quorum sensing (QS) as a cell-to-cell communication system to orchestrate the expression of virulence determinants. The biosynthesis important quinolone signal (PQS) requires pqsABCDE operon. Here, PqsE acts pathway-specific thioesterase, but it also contributes regulation bacterial via an unknown mechanism. In this manuscript, we report discovery inhibitors tool compounds gain further insights into its different functions. Differential scanning fluorimetry (DSF)...
Abstract Hit‐to‐lead optimization is a critical phase in drug discovery. Herein, we report on the fragment‐based discovery and of 2‐aminopyridine derivatives as novel lead‐like structure for treatment dangerous opportunistic pathogen Pseudomonas aeruginosa . We pursue an innovative strategy by interfering with quinolone signal (PQS) quorum sensing (QS) system leading to abolishment bacterial pathogenicity. Our compounds act PQS receptor (PqsR), key transcription factor controlling expression...
Pseudomonas aeruginosa, a prevalent pathogen in nosocomial infections and major burden cystic fibrosis, uses three interconnected quorum-sensing systems to coordinate virulence processes. At variance with other Gram-negative bacteria, one of these relies on 2-alkyl-4(1H)-quinolones (Pseudomonas quinolone signal, PQS) might hence be an attractive target for new anti-infective agents. Here we report crystal structures the N-terminal domain anthranilate-CoA ligase PqsA, first enzyme PQS...
Pseudomonas aeruginosa is a bacterial pathogen that causes life-threatening infections in immunocompromised patients. It produces large armory of saturated and mono-unsaturated 2-alkyl-4(1H)-quinolones (AQs) AQ N-oxides (AQNOs) serve as signaling molecules to control the production virulence factors are involved membrane vesicle formation iron chelation; furthermore, they also have, for example, antibiotic properties. has been shown β-ketoacyl-acyl-carrier protein synthase III (FabH)-like...
Abstract Pseudomonas aeruginosa is a major cause of nosocomial infections and also leads to severe exacerbations in cystic fibrosis or chronic obstructive pulmonary disease. Three intertwined quorum sensing systems control virulence P. aeruginosa, with the rhl circuit playing leading role late infections. The majority traits controlled by transcription factor RhlR depend on PqsE, dispensable thioesterase Quinolone Signal (PQS) biosynthesis4 that interferes through an enigmatic mechanism...