A5054680089- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Probiotics and Fermented Foods
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Respiratory and Cough-Related Research
- Nematode management and characterization studies
- Oral microbiology and periodontitis research
- Gut microbiota and health
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Microbial Natural Products and Biosynthesis
- Nuclear Structure and Function
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
University of California, San Francisco
2014-2022
Universidad Católica de Santa Fe
2021
Summary C andida albicans biofilms are composed of highly adherent and densely arranged cells with properties distinct from those free‐floating (planktonic) cells. These a significant medical problem because they commonly form on implanted devices, drug resistant difficult to remove. . not static structures; rather dynamic develop over time. Here we characterize gene expression in during their development, by comparing them multiple planktonic reference states, identify patterns relevant...
Candida albicans is a fungal species that part of the normal human microbiota and also an opportunistic pathogen capable causing mucosal systemic infections. C. cells proliferate in planktonic (suspension) state, but they form biofilms, organized tightly packed communities attached to solid surface. Biofilms colonize many niches body persist on implanted medical devices, where are major source new Here, we used unbiased global substrate-profiling approach discover proteolytic activities...
ABSTRACT Biofilms are resilient, surface-associated communities of cells with specialized properties (e.g., resistance to drugs and mechanical forces) that distinct from those suspension (planktonic) cultures. Biofilm formation by the opportunistic human fungal pathogen Candida albicans is medically relevant because C. infections highly correlated implanted medical devices, which provide efficient substrates for biofilm formation; moreover, biofilms inherently resistant antifungal drugs....
The nucleocapsid (N) protein of coronaviruses serves two major functions: compaction the RNA genome in virion and regulation viral gene transcription infected cell 1-3 . N contains globular RNA-binding domains surrounded by regions intrinsic disorder 4 Phosphorylation central disordered region is required for normal 5,6 , which occurs a cytoplasmic structure called replication complex (RTC) 7-11 It not known how phosphorylation controls function. Here we show that SARS-CoV-2, together with...
Abstract Robust regulatory signals in the cell often depend on interactions between short linear motifs (SLiMs) and globular proteins. Many of these are poorly characterized because binding proteins cannot be produced amounts needed for traditional methods. To address this problem, we developed a single-molecule off-rate (SMOR) assay based microscopy fluorescent ligand to immobilized protein partners. We used it characterize substrate Anaphase-Promoting Complex/Cyclosome (APC/C), ubiquitin...
Members of the tristetraprolin (TTP) family CCCH tandem zinc finger proteins bind to AU-rich regions in target mRNAs, leading their deadenylation and decay. Family members Saccharomyces cerevisiae influence iron metabolism, whereas single protein expressed Schizosaccharomyces pombe, Zfs1, regulates cell-cell interactions. In human pathogen Candida albicans, deep sequencing mutants lacking orthologous protein, revealed significant increases (> 1.5-fold) 156 transcripts. Of these, 113 (72%)...
Biofilms formed by the human fungal pathogen Candida albicans are naturally resistant to many of antifungal agents commonly used in clinic. We screened a library containing 1600 clinically tested drug compounds identify that inhibit C. biofilm formation. The emerged from initial screen were validated secondary and then for (1) their abilities disrupt mature biofilms (2) synergistic interactions with representatives three most prescribed treat infections, fluconazole, amphotericin B,...
The fungal species Candida albicans is both a member of the human microbiome and pathogen. C. undergoes several different morphological transitions, including one called white-opaque switching. Here, cells reversibly switch between two states, "white" "opaque," each state heritable through many cell generations. Each type has distinct cellular colony morphology they differ in other properties mating, nutritional specialization, interactions with innate immune system. Previous genetic screens...
The human fungal pathogen Candida albicans can form biofilms on biotic and abiotic surfaces, which are inherently resistant to antifungal drugs. We screened the Chembridge Small Molecule Diversity library containing 30,000 “drug-like” small molecules identified 45 compounds that inhibited biofilm formation. These were then tested for their abilities disrupt mature combinatorial interactions with fluconazole, amphotericin B, caspofungin, three drugs most commonly prescribed treat infections....
Abstract Robust regulatory signals in the cell often depend on interactions between short linear motifs (SLiMs) and globular proteins. Many of these are poorly characterized because binding proteins cannot be produced amounts needed for traditional methods. To address this problem, we developed a single-molecule off-rate (SMOR) assay based microscopy fluorescent ligand to immobilized protein partners. We used it characterize substrate Anaphase-Promoting Complex/Cyclosome (APC/C), ubiquitin...