A5005487492- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammasome and immune disorders
- COVID-19 Clinical Research Studies
- Systemic Lupus Erythematosus Research
- Sepsis Diagnosis and Treatment
- Blood disorders and treatments
- S100 Proteins and Annexins
- Platelet Disorders and Treatments
- Inflammatory Myopathies and Dermatomyositis
- Autoimmune and Inflammatory Disorders Research
- Parvovirus B19 Infection Studies
- Dermatological and COVID-19 studies
- Adolescent and Pediatric Healthcare
- Kawasaki Disease and Coronary Complications
- Muscle Physiology and Disorders
- Venous Thromboembolism Diagnosis and Management
- Diabetes and associated disorders
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Intensive Care Unit Cognitive Disorders
- Eosinophilic Disorders and Syndromes
- Immune cells in cancer
- Retinal and Optic Conditions
- Liver Diseases and Immunity
- Acute Lymphoblastic Leukemia research
University of Michigan
2019-2025
Center for Rheumatology
2020-2025
Amgen (United States)
2021
In severe cases of coronavirus disease 2019 (COVID-19), viral pneumonia progresses to respiratory failure. Neutrophil extracellular traps (NETs) are webs chromatin, microbicidal proteins, and oxidant enzymes that released by neutrophils contain infections. However, when not properly regulated, NETs have the potential propagate inflammation microvascular thrombosis — including in lungs patients with acute distress syndrome. We now report sera from COVID-19 elevated levels cell-free DNA,...
Patients with COVID-19 are at high risk for thrombotic arterial and venous occlusions. Lung histopathology often reveals fibrin-based blockages in the small blood vessels of patients who succumb to disease. Antiphospholipid syndrome is an acquired potentially life-threatening thrombophilia which develop pathogenic autoantibodies targeting phospholipids phospholipid-binding proteins (aPL antibodies). Case series have recently detected aPL antibodies COVID-19. Here, we measured eight types...
Abstract Patients with coronavirus disease-19 (COVID-19) are at high risk for thrombotic arterial and venous occlusions. However, bleeding complications have also been observed in some patients. Understanding the balance between coagulation fibrinolysis will help inform optimal approaches to thrombosis prophylaxis potential utility of fibrinolytic-targeted therapies. 118 hospitalized COVID-19 patients 30 healthy controls were included study. We measured plasma antigen levels tissue-type...
Severe cases of coronavirus disease 2019 (COVID-19) are regularly complicated by respiratory failure. Although it has been suggested that elevated levels blood neutrophils associate with worsening oxygenation in COVID-19, is unknown whether drivers the thrombo-inflammatory storm or simple bystanders. To better understand potential role we measured neutrophil activation marker S100A8/A9 (calprotectin) hospitalized patients and determined its relationship to severity illness status. Patients...
ABSTRACT In severe cases of coronavirus disease 2019 ( COVID-19 ), viral pneumonia progresses to respiratory failure. Neutrophil extracellular traps NETs ) are webs chromatin, microbicidal proteins, and oxidant enzymes that released by neutrophils contain infections. However, when not properly regulated, have potential propagate inflammation microvascular thrombosis—including in the lungs patients with acute distress syndrome. While elevated levels blood predict worse outcomes COVID-19, role...
Objective The release of neutrophil extracellular traps (NETs) by hyperactive neutrophils has recently been recognized to play an important role in antiphospholipid syndrome (APS). This study was undertaken evaluate autoantibodies targeting NETs patients with primary APS, and determine their potential functions clinical associations. Methods We measured global anti‐NET activity 76 23 systemic lupus erythematosus without antibodies (aPL), 11 a history unprovoked venous thrombosis aPL, 44...
The release of neutrophil extracellular traps (NETs) by hyperactive neutrophils is recognized to play an important role in the thromboinflammatory milieu inherent severe presentations COVID-19. At same time, a variety functional autoantibodies have been observed individuals with COVID-19, where they likely contribute immunopathology. Here, we aimed determine extent which might target NETs COVID-19 and, if detected, elucidate their potential functions and clinical associations. We measured...
Objective While endothelial dysfunction has been implicated in the widespread thromboinflammatory complications of COVID‐19, upstream mediators endotheliopathy remain, for most part, unknown. This study was undertaken to identify circulating factors contributing cell activation and COVID‐19. Methods Human cells were cultured presence serum or plasma from 244 patients hospitalized with COVID‐19 100 non–COVID‐19–related sepsis. Cell adhesion molecules (E‐selectin, vascular molecule 1,...
ABSTRACT Here, we report on four patients whose hospitalizations for COVID-19 were complicated by venous thromboembolism ( VTE ). All demonstrated high levels of D-dimer as well neutrophil-to-lymphocyte ratios. For three patients, able to test sera neutrophil extracellular trap NET ) remnants and found significantly elevated cell-free DNA, myeloperoxidase-DNA complexes, citrullinated histone H3. Neutrophil-derived S100A8/A9 (calprotectin) was also elevated. Given strong links between...
ABSTRACT Patients with coronavirus disease 19 ( COVID-19 ) are at high risk for thrombotic arterial and venous occlusions. At the same time, lung histopathology often reveals fibrin-based occlusion in small vessels of patients who succumb to disease. Antiphospholipid syndrome APS is an acquired potentially life-threatening thrombophilia which develop pathogenic autoantibodies aPL targeting phospholipids phospholipid-binding proteins. Case series have recently detected COVID-19. Here, we...
Despite its potential to cause significant morbidity in children, pediatric antiphospholipid syndrome (APS) is an understudied condition. In this review, we will cover what known about APS epidemiology and how the clinician might approach diagnosis of APS. We highlight similarities differences with adult disease, both for primary context lupus. Clinical manifestations beyond thrombosis, especially neurologic hematologic nature, be discussed. also consider unique implications...
While endothelial dysfunction has been implicated in the widespread thrombo-inflammatory complications of coronavirus disease-19 ( COVID-19 ), upstream mediators endotheliopathy remain for most part cryptic. Our aim was to identify circulating factors contributing cell activation and COVID-19. Human cells were cultured presence serum or plasma from 244 patients hospitalized with 100 non-COVID sepsis. Cell adhesion molecules (E-selectin, VCAM-1, ICAM-1) quantified by in-cell ELISA. Serum...
ABSTRACT The release of neutrophil extracellular traps ( NETs ) by hyperactive neutrophils is recognized to play an important role in the thromboinflammatory milieu inherent severe presentations COVID-19. At same time, a variety functional autoantibodies have been observed individuals with COVID-19 where they likely contribute immunopathology. Here, we aimed determine extent which might target and, if detected, elucidate their potential functions and clinical associations. We measured...
Pediatric antiphospholipid syndrome (APS) is a thromboinflammatory disease characterized by the presence of circulating antibodies and either thrombotic events or pregnancy morbidity. The objective this study was to review large institution's experience better understand characteristics children with APS.We conducted retrospective pediatric APS at tertiary referral center. electronic medical record system queried from 2000 through 2019, 21 cases were included based on meeting revised Sapporo...
Skin inflammation in juvenile dermatomyositis (JDM) can signal disease onset or flare, and the persistence of cutaneous prevent complete remission. The non-invasive study expression signatures through tape stripping (TS) holds potential to reveal mechanisms underlying heterogeneity organ-specific inflammation. objectives this were 1) define TS lesional non-lesional JDM skin, 2) analyze identify endotypes 3) compare blood signatures. While skin demonstrated interferon signaling as top...
ABSTRACT Severe cases of coronavirus disease 2019 ( COVID-19 ) are regularly complicated by respiratory failure. While it has been suggested that elevated levels blood neutrophils associate with worsening oxygenation in COVID-19, is unknown whether drivers the thrombo-inflammatory storm or simple bystanders. To better understand potential role we measured neutrophil activation marker S100A8/A9 (calprotectin) hospitalized patients and determined its relationship to severity illness status....
ABSTRACT Background Patients with coronavirus disease 19 ( COVID-19 ) are at high risk for thrombotic arterial and venous occlusions. At the same time, bleeding complications have been observed in some patients. Better understanding balance between coagulation fibrinolysis will help inform optimal approaches to thrombosis prophylaxis potential utility of fibrinolytic-targeted therapies. Methods 118 hospitalized patients 30 healthy controls were included study. We measured plasma antigen...
While thrombosis and pregnancy loss are the best-known clinical features of antiphospholipid syndrome (APS), many patients also exhibit "extra-criteria" manifestations, such as thrombocytopenia. The mechanisms that drive APS thrombocytopenia not completely understood, no biomarkers available for predicting antibody (aPL)-mediated Calprotectin is a heterodimer S100A8 S100A9 abundant in neutrophil cytoplasm released upon proinflammatory activation. Here, we sought to evaluate presence,...
Neutrophil hyperactivity and neutrophil extracellular trap release (NETosis) appear to play important roles in the pathogenesis of thromboinflammatory autoimmune disease known as antiphospholipid syndrome (APS). The understanding metabolism has advanced tremendously past decade, accumulating evidence suggests that a variety metabolic pathways guide activities health disease. Our previous work characterizing transcriptome APS neutrophils revealed genes related glycolysis, glycogenolysis,...
Objective Cutaneous inflammation can signal disease in juvenile dermatomyositis (DM) and childhood‐onset systemic lupus erythematosus (cSLE), but we do not fully understand cellular mechanisms of cutaneous inflammation. In this study, used imaging mass cytometry to characterize inflammatory cell populations cell–cell interactions DM as compared cSLE. Methods We performed analysis on skin biopsy samples from patients (n = 6) cSLE 4). Tissue slides were processed incubated with metal‐tagged...