A5051635822- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Crystallography and molecular interactions
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Organic and Molecular Conductors Research
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Magnetism in coordination complexes
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Natural product bioactivities and synthesis
- Immune cells in cancer
- Reproductive System and Pregnancy
- Cytokine Signaling Pathways and Interactions
- Cancer Treatment and Pharmacology
- Organometallic Compounds Synthesis and Characterization
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Breast Cancer Therapies
- Neuropeptides and Animal Physiology
- Estrogen and related hormone effects
- Chemokine receptors and signaling
- Glycosylation and Glycoproteins Research
University of Minnesota
2003-2025
Sichuan University
2025
State Key Laboratory of Oral Diseases
2025
Minneapolis College
2022
University of Minnesota System
2021
Minneapolis Institute of Arts
2021
University of Pittsburgh Medical Center
2019-2020
Community Link
2020
Thrasher Research Fund
2020
Innovative Design Labs (United States)
2017
Transforming growth factor-beta (TGF-beta), a product of neoplastic and hemopoietic cells, is bifunctional regulator the immune response. At femtomolar concentrations, TGF-beta stimulates monocyte migration, picomolar quantities induce synthesis factors, including IL-1, that may promote tissue repair by regulating fibrosis angiogenesis. Paradoxically, at concentrations also blocks ability IL-1 to stimulate lymphocyte proliferation. 0.01 1.0 ng/ml, 1 its homologue, 2, suppress IL-1-dependent...
Transforming growth factor beta (TGF-beta), secreted within an inflammatory site or injected locally, induces leukocyte margination, chemotaxis, and accumulation. In addition to its potent direct chemotactic activity, TGF-beta may promote this response by influencing cell surface integrin expression. At picomolar concentrations, increases steady-state mRNA levels for both the alpha 5 1 chain of fibronectin receptor in human blood monocytes. This increase gene expression is reflected...
Transforming growth factor beta 1 (TGF-β1) and its closely related homologue, TGF-β2, rapidly induce gene expression by freshly isolated human peripheral blood monocytes. Within 3 h of exposure to TGF-β, mRNA species specific for interleukin-1 (IL-1β), tumor necrosis factor-α (TNF-α), platelet-derived (PDGF), basic fibroblast (bFGF) were observed. By 14-18 h, cytokine bioactivity protein detected in the culture supernatants. Furthermore, not only TGF-β1, but also TGF-β2 are expressed...
IL-4 has diverse effects on hematopoietic cells, including the ability to suppress certain mononuclear cell functions. To evaluate effect of evolution acute and chronic arthritis, murine recombinant was administered systemically animals receiving an arthropathic dose group A streptococcal wall fragments. Daily treatment with had a minimal phase, but significantly suppressed chronic, destructive phase. By 4 wk after initiation disease, articular index IL-4-treated reduced > 60% (articular =...
Transforming growth factor beta (TGF-beta) induces leukocyte recruitment and activation, events central to an inflammatory response. In this study, we demonstrate that antagonism of TGF-beta with a neutralizing antibody not only blocks cell accumulation, but also tissue pathology in experimental model chronic erosive polyarthritis. Intraarticular injection monoclonal 1D11.16, which inhibits both 1 2 bioactivity, into animals receiving arthropathic dose bacterial walls significantly...
Abstract Activated mouse N‐ras gene transfected into PC12 rat pheochromocytoma cells suppressed proliferation and promoted neuronal differentiation. Normal in a LTR‐containing vector caused differentiation with reduced efficiency, but normal lacking LTR sequences failed to alter the phenotype. Cultures of NGF‐resistant variant subline U7 also showed outgrowth neurites cessation cell division following transfection mutated ras gene. The present findings suggest that genes can, certain cells,...
Monocytes in the circulation of normal individuals express two receptors for constant region immunoglobulin, Fc gamma RI and RII. In contrast, we have observed that AIDS monocytes significant levels a third R, RIII (CD16), which is normally associated with activation or maturation monocyte population. By dual-fluorescence analysis using monoclonal antibody specific (MAb 3G8), 38.5 +/- 3.2% LeuM3 (CD14)-positive patients were CD16 positive as compared to 10.4 1.0% healthy (n = 29; P less than...
Abstract Transforming growth factor-beta (TGF-beta) is a polypeptide that participates in morphogenesis, inflammation, and would repair. Emerging as an important immunomodulatory molecule, TGF-beta may also regulate the phenotype function of mononuclear cells within inflammatory site. In this study shown to induce expression gp50-65 receptor for Fc gamma RIII on otherwise negative peripheral blood monocytes. Circulating monocytes express RI RII, but not RIII, no inducing agent has previously...
We investigated potential mechanisms by which lymphocytes infiltrating rheumatoid synovium become immunosuppressed. In 20 of 22 synovial fluids and 12 13 tissue culture supernatants, no IL-1 bioactivity could be detected in the thymocyte proliferation assay. These same preparations could, however, support fibroblast monolayers, consistent with presence and/or other growth factors. Addition either or supernatants to exogenous bioassay caused marked inhibition assay indicative an inhibitor....
Although cyclin-dependent kinase 2 (CDK2) is a validated target for both cancer and contraception, developing CDK2 inhibitor with exquisite selectivity has been challenging due to the structural similarity of ATP-binding site, where most inhibitors bind. We previously discovered an allosteric pocket in potential bind selective compound then structurally confirmed anthranilic acid scaffold that binds this high affinity. These are over similar CDK1 show contraceptive potential. Herein, we...
Interleukin (IL) 4 is a multifunctional T cell-derived cytokine that inhibits production and certain effector functions in human monocytes, while enhancing others. We show IL-4 may contribute to the downregulation resolution of an inflammatory response by selectively promoting expression IL-1 receptor antagonist (IL-1ra) blocks action IL-1. IL-1ra specifically binds without initiating signal transduction. Peripheral blood monocytes obtained from cancer patients, before immediately after...
We investigated the effect of recombinant human granulocyte-macrophage CSF (rhGM-CSF) on accessory cell function purified monocytes. Compared with untreated monocytes, rhGM-CSF-treated monocytes promoted enhanced mitogen- and Ag-stimulated lymphocyte proliferation. This enhancement was significantly inhibited by mAb to rhGM-CSF. In experiments designed define mechanism rhGM-CSF augmentation function, shown cause a dose-dependent increase in monocyte expression surface HLA-DR molecules...
Supernatants from human mixed leukocyte cultures or lectin-depleted supernatants of PHA-activated peripheral blood leukocytes were depleted IL 2 by passage over an anti-human rIL2 immunoadsorbent column. The column eluates concentrated, dialyzed, and tested for their ability to synergize with rIL in facilitating cytolytic T lymphocyte (CTL) responses allogeneic, uv-irradiated HT144 melanoma cells vitro. CTL generated the presence 1 X 10(-4) M hydrocortisone sodium succinate order minimize...
As a multifunctional cytokine that can augment certain T cell responses, IL-4 is being evaluated currently as possible therapeutic agent in the treatment of cancer patients. In this report, PBMC were isolated from such patients before and after therapy analyzed for phenotypic functional alterations. Differential blood counts showed relative percentages lymphocytes and, to lesser degree, monocytes decreased with IL-4. However, when phenotypically by FACS, monocyte numbers generally increased,...
The retinoic acid receptor alpha (Rarα) has been validated as a male contraceptive target by genetic knockouts resulting in sterility. effects on spermatogenesis the absence of RARα resemble loss RAR signaling vitamin A deficiency, and mice are otherwise normal. animals treated orally with dual RARα/RARγ antagonist BMS-189453 closely phenocopies function. Notably, sterility is reversible. We, therefore, wished to identify RARα−selective inhibitors for potential non-hormonal contraception....
Recently, invisible appliances have become popular due to their aesthetics and comfort, but impact on periodontal health in periodontitis patients has not been fully elucidated. The purpose of this study was investigate the effectiveness fixed appliance treatment for experimental rats while monitoring dynamics Th17/Treg cells osteoclast-related cytokines during treatment. SD were randomly divided into six groups: control, model, scaling-0 (basic treatment), no appliance, appliance. After...
Mononuclear phagocytes in the synovium of patients with arthritis, contrast to blood monocytes, were found express a third receptor for constant region Ig (Fc gamma RIII), addition Fc RI and RII. Previously identified on mature mononuclear or exposed transforming growth factor-beta (TGF-beta) vitro, this study documents presence RIII (CD16) expressing cells at an inflammatory site. Furthermore, CD16 majority LeuM3 (CD14) positive synovial monocytic could be mimicked by exposing monocytes...
N-nitrosopiperidine (NPIP) is a potent esophageal carcinogen in rats whereas structurally similar N-nitrosopyrrolidine (NPYR) induces liver, but not tumors. NPIP possible causative agent for human cancer. Our goal to explain mechanistically these differing carcinogenic activities the esophagus. We hypothesize that differences metabolic activation of nitrosamines could be one factor accounting their carcinogenicity. alpha-Hydroxylation key pathway leading nitrosamine-induced carcinogenesis....