A5101418646- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Myasthenia Gravis and Thymoma
- Peptidase Inhibition and Analysis
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Complement system in diseases
- Protein purification and stability
- Advanced Biosensing Techniques and Applications
- Prostate Cancer Treatment and Research
- Lung Cancer Research Studies
- Machine Learning in Bioinformatics
- Lipid Membrane Structure and Behavior
- Neuropeptides and Animal Physiology
- Peroxisome Proliferator-Activated Receptors
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Viral Infectious Diseases and Gene Expression in Insects
- Adrenal Hormones and Disorders
University of Debrecen
2013-2025
The Francis Crick Institute
2025
BioSystem (Germany)
2015
Pfizer (France)
2007
Roche (France)
2005
Pfizer (United States)
2002
Amgen (United States)
1995-1996
National Institute on Alcohol Abuse and Alcoholism
1992-1995
National Institutes of Health
1990-1995
University of Iowa
1994
Abstract A new monoclonal antibody (mAb), named 3.9, is described that specific for the p150,95 molecule, a member of LFA‐1, CR3, family human leukocyte differentiation antigens. The LFA‐1 molecule participates in variety T cell interactions and CR3 receptor complement component iC3b, but little known about molecule. Here we show expression confined to myeloid cells. mAb 3.9 reacts variably with neutrophils, more strongly monocytes most expressed on tissue macrophages. Using this others,...
IL-1 is a cytokine with wide variety of effects on cells involved in inflammatory and immune responses, hemopoiesis, bone formation. Many cell types have been shown to produce vitro; however, very little known about the source role vivo. By using situ hybridization, we examined tissue distribution containing mRNA normal C57BL/6 mice. The results show that many organs contain mRNA-positive cells, but highest frequency was found lymphoid organs. localization these suggest mRNA-producing are...
TNF-alpha is a small peptide cytokine produced primarily by activated macrophages. One of the many biologic activities TNF killing diverse types tumor cells. We considered possibility that was mediated itself at an intracellular site, subsequent to receptor-mediated endocytosis. To test this hypothesis, we microinjected into various murine normal cells and cell lines, some which were killed given usual extracellular route, others not. Cytotoxic effects observed in several 2 4 h after...
A challenge in the treatment of lung cancer is lack early diagnostics. Here, we describe application monoclonal antibody proteomics for discovery a panel biomarkers detection (stage I) non-small cell (NSCLC). We produced large libraries directed against natural form protein antigens present plasma NSCLC patients. Plasma associated with presence were detected via high throughput ELISA. Differential profiling proteomes four clinical cohorts, totaling 301 patients and 235 healthy controls,...
<i>Background:</i> Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory characterized by progressive airflow limitation and significant extrapulmonary (systemic) effects that lead to co-morbid conditions, though the pathomechanism of COPD largely undetermined. Alveolar macrophages (AM) derived from peripheral monocytes (MO) appear play key role in initiating and/or sustaining progression. <i>Objectives:</i> To identify disease- cell type-specific...
The presence of prolactin (PRL) mRNA in the mammary gland, placenta, and pituitary gland lactating pregnant rats was investigated by polymerase chain reaction (PCR). Polyadenylated RNA prepared from total samples oligo(dT)-cellulose chromatography, complementary cDNAs were synthesized. A standardized amount cDNA each sample used as template a Taq PCR under high-stringency conditions. amplified signal with predicted size approximately 375 bp glands rats, placentae rats. This band specifically...
We have analyzed the involvement of IL 1 in a cellular interaction during which murine B cells serve as antigen-presenting for T cell clone (D10.G4.1). This was chosen study because it is highly dependent on exogenous concanavalin A (or anti-receptor antibody)-induced proliferation. observed that presented either self I-Ak plus nominal antigen or allogeneic I-Ab, and consequently induced proliferation this without any requirement 1. After an overnight co-culture with allogenic cells, we...
A series of sequential events orchestrates cell growth and division, set in motion by cyclin-dependent kinases (Cdks). In the "qualitative model" for Cdk control, order is achieved cycle stage-specific cyclins. However, single-cyclin cells retain order. an alternative "quantitative model," increasing activity triggers substrate phosphorylation at thresholds. Here, we test a key prediction from quantitative model: best substrates should be first to phosphorylated. Phosphoproteome analysis...
Circulating tumor cell enrichment and enumeration are advancing early detection of cancer, monitoring therapy response, even next-generation therapies. Efficiently capturing rare cells from complex biological fluids is essential in both diagnostic therapeutic applications. EpCAM-positive specifically captured by utilizing covalently immobilized anti-EpCAM monoclonal antibodies onto the surface chemically modified glass microbeads. To maximize capture efficiency, bead geometry, immobilization...
A CE‐based method was introduced to compare the N ‐glycosylation profile of haptoglobin in normal and pathologic conditions. To assess biomarker potential glycosylation changes various lung diseases, isolated from plasma samples healthy, pneumonia, chronic obstructive pulmonary disease, cancer patients by means two haptoglobin‐specific monoclonal antibodies. Haptoglobin ‐glycans were then enzymatically released, fluorescently labeled, profiled CE. Disease‐associated core antennary...
Thymic involution is thought to be an important factor of age related immunodeficiency. Understanding the molecular mechanisms human thymic senescence may lead discovery novel therapeutic approaches aimed at reestablishment central and peripheral T cell repertoire. As initial approach, here we report that decline FOXN1 transcription correlates with age, while other genes, DLL1, DLL4 WNT4, essential for thymopoiesis, are constitutively transcribed. Using a epithelial line (hTEC), show...
Abstract During embryonic development of the mouse, before expressing classical T cell markers, blast cells colonizing thymus react with monoclonal antibody AMT‐13 shown previously to detect interleukin 2 receptors. The proportion + decreases as gestation time increases. On other hand, Thy‐1 , Lyt‐1 and Lyt‐2 increases during ontogenesis. 19th day when architecture is comparable adult thymus, become localized in subcapsular area cortex. In after cortison treatment regenerating express...
In addition to smoking, genetic predisposition is believed play a major role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Genetic association studies new candidate genes COPD may lead improved understanding disease.Two proposed casual single nucleotide polymorphisms (SNP) (rs1051740, rs2234922) microsomal epoxide hydrolase (EPHX1) and three SNPs (rs1801282, rs1800571, rs3856806) peroxisome proliferator-activated receptor gamma (PPARG), gene, were genotyped...
Fetal pre-T cells express low-affinity receptors for IgG (Fc gamma R) at a developmental stage prior to the rearrangement and expression of immunoglobulin genes. The present studies investigated possible functional significance Fc R on fetal cells. Between 13 17 days development subpopulation T-cell receptor-, Thy-1+ thymocytes R. same contain mRNA several forms RII beta 1, 2, RIII). Concurrently, Pgp-1-, Thy-1-, surface-immunoglobulin- thymic cell binds recombinant soluble In principle this...
Four monoclonal antibodies (BH11, T2/30, AG3, and BC3) were produced against different epithelial components of the normal thymus. An immunohistologic study was performed on 13 thymomas by use these other stromal lymphocyte-specific reagents. The aim this to find possible relationships between proliferating thymoma cell type T composition thymomas. Our results indicate that cortical differentiation is present in thymomas, induced absence detectable levels MHC class II antigens component most...
One-step global profiling of analyte (mRNA, protein, metabolite) biomarkers may soon replace conventional blood and histological/biopsy diagnostics technologies. It is important to establish whether the numerous other body fluid-derived potential novel will be sufficiently efficacious precise replace, for example, imaging functional diagnostic tests. Currently, technologies spirometry are indispensable diagnosis management chronic obstructive pulmonary disease (COPD). To validate concept...
Molecular heterogeneity of mAb preparations is the result various co- and post-translational modifications to contaminants related production process. Changes in molecular composition results alterations functional performance, therefore quality control validation therapeutic or diagnostic protein products essential. A special case consistent libraries (QuantiPlasma™ PlasmaScan™) for proteome profiling, which represents a challenge because high number mAbs (>1000). Here, we devise generally...
Fixation of APC with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (ECDI) eliminates their ability to stimulate proliferation alloreactive T cells or the D10 cell clone, although a partial response, IL-4 production, was measured. However, if were activated before fixation, they could be ECDI-fixed and retain induce proliferation. IL-1, LPS capable activating in this way, whereas IFN-gamma not. This activation step occurred 6 h, required protein synthesis, distinct from increases Ia IL-1....
IL-2R alpha-chain is expressed on a subset of mouse CD4- and CD8-, double negative (DN) thymocytes. This expression some DN thymocytes in the has led to proposal that IL-2 might serve as principal growth and/or differentiation factor for immature However, previous histologic observations have indicated not subcapsular thymic blasts (an area rich cells) either huma or rat thymus, whereas all three species display few cells medulla. Therefore, we characterized determine whether they contained...
A mass spectrometric (MS)-based strategy for antigen (Ag) identification and characterization of globally produced monoclonal antibodies (mAbs) is described. Mice were immunized with a mixture native glycoproteins, isolated from the pooled plasma patients nonsmall cell lung cancer (NSCLC), to generate library IgG-secreting hybridomas. Prior immunization, NSCLC was subjected 3-sequential steps affinity fractionation, including high abundant protein depletion, glycoprotein enrichment,...
Current proteomic technologies focus on the quantification of protein levels, while little effort is dedicated to development system approaches simultaneously monitor proteome variability and abundance. Protein variants may display different immunogenic epitopes detectable by monoclonal antibodies. Epitope results from alternative splicing, posttranslational modifications, processing, degradation, complex formation possesses dynamically changing availability interacting surface structures...