A5087061295- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Advanced Electron Microscopy Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Glycosylation and Glycoproteins Research
- Nanopore and Nanochannel Transport Studies
- Protein purification and stability
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Chemical and Physical Properties in Aqueous Solutions
- Calcium signaling and nucleotide metabolism
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Force Microscopy Techniques and Applications
- Connexins and lens biology
- Advanced X-ray Imaging Techniques
- Crystallization and Solubility Studies
- Advanced Biosensing Techniques and Applications
- Magnesium in Health and Disease
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroscience and Neuropharmacology Research
- Antimicrobial agents and applications
- Neonatal Respiratory Health Research
- Near-Field Optical Microscopy
University of Lisbon
1994-2025
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2017-2021
Italian Institute of Technology
2017-2020
Instituto Superior Técnico
1994-2016
Center for NanoScience
2016
Instituto Superior de Tecnologias Avançadas
2013
A robust and efficient cellular response to lysosomal membrane damage prevents leakage from the lysosome lumen into cytoplasm. This is understood happen through either repair or lysophagy. Here we report exocytosis as a third mechanism damage, which further potentiated when degradation mechanisms are impaired. We show that Connexin43 (Cx43), protein canonically associated with gap junctions, recruited plasma damaged lysosomes, promoting their secretion accelerating cell recovery. The effects...
Abstract Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect chromatin folding on genome function. Recent developments super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with sub-diffraction spatial resolution required image at nanoscale. Here, we present a method enhance stimulated-emission depletion (STED) microscope based only modulation STED intensity...
Aquaporins are membrane channels responsible for the bidirectional transfer of water and small non-charged solutes across cell membranes. AQP3 AQP5 overexpressed in pancreatic ductal adenocarcinoma, playing key roles migration, proliferation, invasion. Here, we evaluated involvement biomechanical properties, cell-cell adhesion, following a loss-of-function strategy on BxPC-3 cells.Silencing was functionally validated by reduced permeability had implications slowing wound recovery. Moreover,...
As a surface-located molecule in biological membranes, cholesterol sulphate (CholS) plays major role membrane-driven cell–cell processes and events including platelet-cell adhesion, T-cell receptor signalling, sperm–egg interaction, membrane fusion, skin differentiation. Despite this, little is known about the biophysical implications of CholS at cells organelles. In this study, we investigate effect increasing content on properties cholesterol-poor cholesterol-rich biomimetic models. Data...
Deciphering the spatiotemporal coordination between nuclear functions is important to understand its role in maintenance of human genome. In this context, super-resolution microscopy has gained considerable interest because it can be used probe spatial organization functional sites intact single-cell nuclei 20-250 nm range. Among methods that quantify colocalization from multicolor images, image cross-correlation spectroscopy (ICCS) offers several advantages, namely does not require a...
Klebsiella pneumoniae, one of the most common pathogens found in hospital-acquired infections, is often resistant to multiple antibiotics. In fact, multidrug-resistant (MDR) K. pneumoniae producing KPC or OXA-48-like carbapenemases are recognized as a serious global health threat. this sense, we evaluated virulence KPC(+) OXA-48(+) aiming at potential antimicrobial therapeutics. carbapenemase (KPC) and expanded-spectrum oxacillinase OXA-48 isolates were obtained from patients treated medical...
Although the evidence for presence of functionally important nanosized phosphorylated phosphoinositide (PIP)-rich domains within cellular membranes has accumulated, very limited information is available regarding structural determinants compartmentalization these phospholipids. Here, we used a combination fluorescence spectroscopy and microscopy techniques to characterize differences in divalent cation-induced clustering PI(4,5)P2 PI(3,5)P2. Through methodologies were able detect efficiency...
Gangliosides are important glycosphingolipids involved in a multitude of physiological functions. From physicochemical standpoint, this is related to their ability self-organize into nanoscopic domains, even at molar concentrations one per 1000 lipid molecules. Despite recent experimental and theoretical efforts suggesting that hydrogen bonding network crucial for nanodomain stability, the specific ganglioside moiety decisive development these nanodomains has not yet been identified. Here,...
Giant unilamellar vesicles (GUVs) with phase coexistence allow for the recovery of inter-domain partition coefficients (<italic>K</italic><sub>p</sub>) fluorescent molecules through comparison fluorescence intensities in each phase.
Abstract A robust cellular response to lysosomal membrane damage is essential prevent content leakage the cytoplasm and subsequent activation of cell death pathways. Here we report exocytosis as an important mechanism damage, which further potentiated when repair or degradation mechanisms are impaired. Our data reveal that Connexin43 (Cx43), a protein canonically associated with gap junctions, recruited damaged lysosomes promote their secretion, thereby accelerating recovery. The exocytotic...
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P