A5004719404- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Reproductive Health and Technologies
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Effects and risks of endocrine disrupting chemicals
- Kruppel-like factors research
- Animal Genetics and Reproduction
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Urological Disorders and Treatments
- Birth, Development, and Health
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
- Biomedical Ethics and Regulation
- Toxic Organic Pollutants Impact
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Nitric Oxide and Endothelin Effects
- TGF-β signaling in diseases
- Molecular Biology Techniques and Applications
Masaryk University
2016-2025
University Hospital Brno
2015-2025
Czech University of Life Sciences Prague
2005-2024
Poznan University of Medical Sciences
2021
Veterinary Research Institute
2009-2019
Nicolaus Copernicus University
2018
Laboratoire des signaux et systèmes
2012
University of Agriculture Faisalabad
2005
Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information available on effects of ovarian follicles. In our study, we demonstrated presence in follicular fluid women at a concentration 22.4 nM. The effect such concentrations oocyte maturation and subsequent embryo development still unknown. Therefore, focused vitro maturation, fertilization, development. As model, used porcine oocytes, which show many physiological similarities human...
Exposure to bisphenols has been found have adverse effects on male reproductive function in animals. Human exposure is widespread. Bisphenol A (BPA) and its analogues, including bisphenol S (BPS), F (BPF), AF (BPAF) are utilized various consumer products such as food contact materials dental resins. The of these compounds fertility spermatogenesis unclear findings from human studies inconsistent. In this cross-sectional study, we evaluated the influence BPA, BPS, BPF, BPAF (BPs) measured...
Abstract In patients with testicular germ cell tumours (TGCT), sperm cryopreservation prior to anti-cancer treatment represents the main fertility preservation approach. However, it is associated low recovery rate after thawing. Since a high-energy demanding cell, which supplied by glycolysis and oxidative phosphorylation (OXPHOS), mitochondrial dysfunctionality can directly result in anomalies. this study, we investigated bioenergetic pattern of cryopreserved TGCT comparison normozoospermic...
It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent to study this phenomenon, we employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), the frequency of aneuploidy in porcine oocytes, rare cells obtained aged...
Abstract Folliculogenesis is the process of ovarian follicle formation,, taking presence during foetal period. During follicular development, oogoniums undergo meiosis and oocytes are formed. In ovaries new born sows, primary secondary follicles present and, 90 days after birth, tertiary appear. development in growth granulosa cells differentiation thecal can be observed. A cavity filled with fluid appears. Granulosa divided into: mural corona radiata, which together oocyte form cumulus...
Because of the deep involvement granulosa cells in processes surrounding cycles menstruation and reproduction, there is a great need for deeper understanding ways which they function during various stages those cycles. One main influence numerous sex associated hormonal interaction. Expression steroid hormones influences range both primary secondary sexual characteristics, as well regulate oogenesis, folliculogenesis, ovulation, pregnancy. Understanding exact molecular mechanisms underlying...
Abstract The pig is a polyestrous animal in which the ovarian cycle lasts about 21 days and results ovulation of 10-25 oocytes. Ovum reaches 120-150 μm diameter, with surrounding corona radiata providing communication environment. zona pellucida composed glycoproteins: ZP1, ZP2, ZP3. In course oogenesis, RNA protein accumulation for embryonic development occurs. Maternal mRNA template production. Nuclear, cytoplasmic genomic maturity condition ability ovum to undergo fertilization. There are...
Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary involve not only maturing germ cell, but also few types somatic cells that assist ovarian mediate dialog with oocyte. These cells, granulosa theca, are heavily involved essential reproductive processes, such as ovulation, fertilization, embryo implantation. In this study, we used expressive microarray approach to analyze transcriptome porcine during short-term...
The human ovarian granulosa cells (GCs) surround the oocyte and form proper architecture of follicle. ability GCs to proliferate differentiate in conditions vitro culture has been proven. However, there is still a large field for extensive investigation molecular basics, as well marker genes, responsible these processes. This study aimed find new encoding proteins that regulate capability proliferation differentiation during long-term primary culture. follicular were collected from...
The growth and development of oocyte affect the functional activities surrounding somatic cells. These cells are regulated by various types hormones, proteins, metabolites, regulatory molecules through gap communication, ultimately leading to maturation oocytes. close association between oocytes, which together form cumulus-oocyte complexes (COCs), their bi-directional communication crucial for acquisition developmental competences oocyte. In this study, oocytes were extracted from ovaries...
The physiological processes that drive the development of ovarian follicle, as well process oogenesis, are quite known. Granulosa cells major players in this occurrence, being somatic element female gamete development. They participate directly building cumulus-oocyte complex surrounding ovum. In addition to that, they have a further impact on reproductive processes, place steroid sex hormone synthesis and secretion. It is known follicle creates need for angiogenesis blood vessel ovary....
Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well‑known, its exact molecular mechanisms remain widely undiscovered. growth, differentiation therefore subject scientific debate. Precious literature has indicated that oocyte itself serves a regulatory role in underlying processes. Hence, present study performed expression microarrays to analyze complete transcriptome...
Summary After in vitro maturation, the unfertilized pig oocytes underwent process called ageing. This involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specific inhibitor trichostatin A (TSA), significantly delayed maturation cultured . The ageing matured under effect TSA is same without TSA. inhibition deacetylase during oocyte reduced percentage fragmented (from 30% untreated to 9% aged 100 nM TSA)....
The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation transformation granulosa cells (GCs) during folliculogenesis, oogenesis, oocyte maturation. Although role 17 beta-estradiol (E2) ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, GCs-luteal requires further research. Therefore, goal this study was to assess real-time porcine GCs vitro relation...
Abstract Constant advances in medicine, both human and veterinary, lead to continuous discovery of new drugs treatments. Recently, the aspect stem cell use regenerative medicine has been very popular. There are still too few clinical trials on animals that could precisely estimate therapeutic efficacy therapy. However, cells a source extraordinary potential for multiplication differentiation which, if used properly, can prove be an effective mean treatment numerous diseases currently...
In this paper we assessed: (i) the change in nitric oxide synthase (NOS) isoforms' expression and intracellular localization NOS mRNA porcine oocytes during meiotic maturation; (ii) effect of inhibition by N(omega)-nitro-l-arginine methyl ester (l-NAME) aminoguanidine (AG) on maturation cumulus-oocyte complexes (COC) as well denuded (DO); (iii) (NO) formation COC. All three isoforms (eNOS, iNOS nNOS) (eNOS mRNA, nNOS mRNA) were found both their cumulus cells except for which was not detected...
Maturation of cumulus-oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation differentiation cumulus cells. The migration COCs to the oviduct after ovulation interaction between female gametes and/or embryos with maternal tissues still poorly recognized on molecular level. This study was aimed first demonstrate mRNA expression profile cell markers during different stages porcine...
Exposure to bisphenols is related negative effects on male reproduction. The exposure associated with several modes of action including impact the blood-testis barrier (BTB) in testes or direct effect spermatozoa. Bisphenols have been detected human seminal plasma, but possible mechanism transfer not clear. Some authors consider through be crucial. Therefore, this work, we compared normozoospermic men and after vasectomy who interrupted vas deferens their ejaculate does contain testicular...
Cervical mucus (CM) is a viscous fluid that produced by the cervical glands and functions as uterine cervix plug. Its viscosity decreases during ovulation, providing window for non-invasive sampling. This study focuses on proteomic characterization of CM to evaluate its potential non-invasively acquired source biomarkers in understanding molecular (patho)physiology female genital tract. The first objective this work was optimize experimental workflow processing second assess differences...