A5039768383- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Viral Infections and Vectors
- Virus-based gene therapy research
- Microbial infections and disease research
- HIV Research and Treatment
- Agriculture and Farm Safety
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Respiratory viral infections research
- interferon and immune responses
- T-cell and Retrovirus Studies
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Yersinia bacterium, plague, ectoparasites research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral-associated cancers and disorders
- Antimicrobial Peptides and Activities
Canadian Food Inspection Agency
2016-2025
Canadian Science Centre for Human and Animal Health
2016-2025
University of Manitoba
2020-2024
University of Calgary
2018-2024
Animal and Plant Health Inspection Service
2023
Government of Canada
2021
General Hospital of Attica
2021
Ministry of Food and Agriculture
2021
Vietnam National University of Agriculture
2020
University of Georgia
2019
Abstract Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential provide decentralized and low-cost molecular diagnostics. However, it remains a challenge deliver this sensing capacity into hands of users practical manner. Here, we leverage glucose meter, one most widely available point-of-care devices, serve as universal reader for these We describe translator that can convert activation conventional output be read by off-the-shelf...
The African swine fever virus (ASFV) is currently causing a world-wide pandemic of highly lethal disease in domestic and wild boar. Currently, recombinant ASF live-attenuated vaccines based on genotype II strain are commercially available Vietnam. With 25 reported ASFV genotypes the literature, it important to understand molecular basis usefulness genotyping, as well true significance epidemiology, transmission, evolution, control, prevention ASFV. Historically, genotyping was used for...
African swine fever virus (ASFV) genotype II is endemic to Vietnam. We detected recombinant ASFV genotypes I and (rASFV I/II) strains in domestic pigs from 6 northern provinces The introduction of rASFV I/II could complicate ongoing control measures the region.
African swine fever virus (ASFV) recombinant strains pose new challenges for diagnosis and control. This study characterizes genotype I II ASFV identified in northern Vietnam 2023 through whole-genome sequencing comparative genomic analysis. Seven ASFV-positive samples from six provinces were analyzed, with detected Bac Giang, Phu Tho, Vinh Phuc provinces. Isolates showed hemadsorption positivity despite having B646L, indicating their nature. Genome-wide analysis revealed 19 recombination...
African swine fever (ASF) is a high-consequence transboundary hemorrhagic of swine. It continues to spread across the globe causing socio-economic issues and threatening food security biodiversity. In 2020, Nigeria reported major ASF outbreak, killing close half million pigs. Based on partial sequences genes B646L (p72) E183L (p54), virus responsible for outbreak was identified as an (ASFV) p72 genotype II. Here, we report further characterization ASFV RV502, one isolates obtained during...
African swine fever (ASF) is a lethal disease of domestic pigs that has been causing outbreaks for over century in Africa ever since its first discovery 1921. Since 1957, there have sporadic outside Africa; however, no outbreak as devastating and far-reaching the current pandemic originated from 2007 Republic Georgia. Derivatives with high degree similarity to progenitor strain, ASFV-Georgia/2007, sequenced various countries Europe Asia. However, strains circulating are largely unknown, 24...
In this paper, we report the characterization of a genetically modified live-attenuated African swine fever virus (ASFV) field strain isolated from Vietnam. The isolate, ASFV-GUS-Vietnam, belongs to p72 genotype II, has six multi-gene family (MGF) genes deleted, and an Escherichia coli GusA gene (GUS) inserted. When 6–8-week-old pigs were inoculated with ASFV-GUS-Vietnam oro-nasally (2 × 105 TCID50/pig), they developed viremia, mild fever, lethargy, inappetence, shed in their oral nasal...
The sustained spread of African swine fever (ASF) virus throughout much the world has made ASF a global animal health priority, with an increased emphasis on enhancing preparedness to prevent, detect and respond potential outbreak (ASFV). In event ASFV entry North American population, enhanced surveillance diagnostic testing strategies will be critical facilitate progressive response eradication disease. Compared individual sampling, pen-based oral fluid collection for active is non-invasive...
In 2007, an outbreak of African swine fever (ASF), a deadly disease domestic and wild boar caused by the virus (ASFV), occurred in Georgia has since spread globally. Historically, ASFV was classified into 25 different genotypes. However, newly proposed system recategorized all isolates 6 genotypes exclusively using predicted protein sequences p72. large genome that encodes between 150-200 genes, classifications single gene are insufficient misleading, as strains encoding identical p72 often...
The putative receptor proteins of Pasteurella haemolytica leukotoxin were isolated from bovine polymorphonuclear neutrophil lysate by affinity chromatography on a leukotoxin-specific monoclonal antibody column to which the was pre-bound. SDS-PAGE purified showed four bands at 180 kDa, 170 150 kDa and 95 in addition expected 102-kDa band series with molecular masses lower than 102 representing disintegrated leukotoxin. N-terminal amino acid sequencing 170-kDa homology human murine CD11b....
ABSTRACT Varicella-zoster virus (VZV) open reading frame 63 (ORF63) is the most abundant transcript expressed during latency in human sensory ganglia. VZV with ORF63 deleted impaired for replication melanoma cells and fibroblasts rodents. We found that of deletion mutant fully complemented U2OS cells, which have been shown to complement growth herpes simplex type 1 (HSV-1) ICP0 mutants. Since HSV-1 mutants are hypersensitive alpha interferon (IFN-α), we examined effect IFN-α on replication....
Porcine respiratory disease complex (PRDC) is one of the most important health concerns for pig producers and can involve multiple viral bacterial pathogens. No simple, single-reaction diagnostic test currently exists simultaneous detection major pathogens commonly associated with PRDC. Furthermore, implicated in PRDC requires time-consuming culture-based methods that take several days to obtain results. In this study, a novel prototype automated microarray integrates automates all steps...
: African swine fever virus (ASFV) continues to spread globally, causing severe economic losses pig farmers. Vietnam licensed two live attenuated vaccines based on the ASFV strains ASFV-G-ΔI177L and ASFV-G-ΔMGF control ongoing ASF outbreaks. In 2023, newly emerging highly virulent recombinant viruses (rASFV I/II) containing genetic elements from both p72 genotype I II were reported Northern Vietnam.
ABSTRACT Leukotoxin (Lkt) secreted by Mannheimia ( Pasteurella ) haemolytica is an RTX toxin which specific for ruminant leukocytes. Lkt binds to β 2 integrins on the surface of bovine have a common subunit, CD18, that associates with three distinct α chains, CD11a, CD11b, and CD11c, give rise different integrins, CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (CR4), respectively. Our earlier studies revealed all suggesting may be receptor Lkt. In order unequivocally elucidate role CD18...
Foot-and-mouth disease (FMD) is a highly contagious viral of cloven-hoofed animals, which can decimate the livestock industry and economy countries previously free this disease. Rapid detection foot-and-mouth virus (FMDV) critical to containing an FMD outbreak. Availability rapid, sensitive specific, yet simple field-deployable assay would support local decision-making during FMDV Here we report validation novel reverse transcription-insulated isothermal PCR (RT-iiPCR) that be performed on...
Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the industry. User-friendly, sensitive, rapid diagnostic tests utilize low-cost field-deployable instruments for CSF diagnosis be useful surveillance and outbreak monitoring. In this study, we describe validation of new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay detection classical virus (CSFV) compact, user-friendly device (POCKIT™ Nucleic Acid Analyzer) does not need data...
African swine fever virus (ASFV) continues to spread globally causing severe economic losses the pig farmers. Vietnam licensed two live attenuated vaccines based on ASFV strains ASFV-G-ΔI177L and ASFV-G-ΔMGF, control ongoing ASF outbreaks. In 2023, newly emerging highly virulent recombinant viruses containing genetic elements from both p72 genotype I II (rASFV I/II) were reported Northern Vietnam. This study evaluated if vaccine able protect pigs against rASFV I/II strain...
Abstract The objectives of this study were to identify the mechanism(s) pseudorabies virus (PrV)-induced down-regulation porcine class I molecules and viral protein(s) responsible for effect. ability PrV interfere with peptide transport activity TAP was determined by an in vitro assay. In assay, kidney (PK-15) cells permeabilized streptolysin-O incubated a library 125I-labeled peptides having consensus motifs glycosylation endoplasmic reticulum (ER). efficiency from cytosol into ER adsorbing...
Varicella-zoster virus (VZV) immediate-early 63 protein (IE63) is abundantly expressed during both acute infection in vitro and latent human ganglia. Using the yeast two-hybrid system, we found that VZV IE63 interacts with antisilencing function 1 (ASF1). ASF1 a nucleosome assembly factor which member of H3/H4 family histone chaperones. coimmunoprecipitated colocalized transfected cells expressing VZV-infected cells. also lytic latently enteric neurons. exists two isoforms, ASF1a ASF1b,...