A5005716784- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Chromium effects and bioremediation
- Metal Extraction and Bioleaching
- Marine and coastal ecosystems
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Vector-Borne Animal Diseases
- Trace Elements in Health
- Sulfur Compounds in Biology
- Head and Neck Cancer Studies
- Metabolomics and Mass Spectrometry Studies
- Hydrocarbon exploration and reservoir analysis
- Salmonella and Campylobacter epidemiology
- Legume Nitrogen Fixing Symbiosis
- Infections and bacterial resistance
- Virus-based gene therapy research
- Plant Pathogens and Fungal Diseases
- Polar Research and Ecology
- Water Treatment and Disinfection
University of Copenhagen
2022-2024
Planta
2023
Bose Institute
2012-2022
South Dakota State University
2019-2022
Western University of Health Sciences
2020-2022
Government Medical College and Hospital
2022
NaCl-saturated brines such as saltern crystalliser ponds, inland salt lakes, deep-sea and liquids-of-deliquescence on halite are commonly regarded a paradigm for the limit of life Earth. There are, however, other habitats that thermodynamically more extreme. Typically, environments contain all domains perform complete biogeochemical cycling. Despite their reduced water activity, ∼0.755 at 5 M NaCl, some halophiles belonging to Archaea Bacteria exhibit optimum growth/metabolism in these...
A major function of the gut microbiota is to provide colonization resistance, wherein pathogens are inhibited or suppressed below infectious levels. However, fraction required for resistance remains unclear. We used culturomics isolate a culture collection comprising 1,590 isolates belonging 102 species. This represents 34.57% taxonomic diversity and 70% functional capacity, as estimated by metagenomic sequencing fecal samples culture. Using whole-genome sequencing, we characterized species...
Little is known about life in the boron-rich hot springs of Trans-Himalayas. Here, we explore geomicrobiology a 4438-m-high spring which emanates ~70 °C-water from boratic microbialite called Shivlinga. Due to low atmospheric pressure, vent-water close boiling point so can entropically destabilize biomacromolecular systems. Starting vent, Shivlinga's was revealed along thermal gradients an outflow-channel and progressively-drying mineral matrix that has no running water; ecosystem...
Fusobacterium necrophorum is a Gram-negative, filamentous anaerobe prevalent in the mucosal flora of animals and humans. It causes necrotic infections cattle, resulting substantial economic impact on cattle industry. Although infection severity management differ within F. species, little known about speciation genetic virulence determinants between strains. To characterize clinical isolates, we performed whole-genome sequencing four bovine isolates (8L1, 212, B17, SM1216) one human isolate...
Biogeochemistry of oxygen minimum zone (OMZ) sediments, which are characterized by high input labile organic matter, have crucial bearings on the benthic biota, gas and metal fluxes across sediment-water interface, carbon-sulfur cycling. Here we couple pore-fluid chemistry comprehensive microbial diversity data to reveal sedimentary cycle a water-depth transect covering entire thickness eastern Arabian Sea OMZ, off west coast India. Geochemical show remarkable increase in average total...
To understand SARS-CoV-2 microevolution, this study explored the genome-wide frequency, gene-wise distribution, and molecular nature of all point-mutations detected across its 71,703 RNA-genomes deposited in GISAID till 21 August 2020. Globally, nsp1/nsp2 orf7a/orf3a were most mutation-ridden non-structural structural genes respectively. Phylogeny 4618 spatiotemporally-representative genomes revealed that entities belonging to early lineages are mostly spread over Asian countries, including...
ABSTRACT The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal bacterial communities along ∼3 m sediment-horizons the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath sea-level) and 580 were at 15–30 cm intervals, using metagenomics, pure-culture-isolation, genomics metatranscriptomics. Genes for respiration, oxidation...
Abstract To elucidate how geothermal irregularities affect the sustainability of high-temperature microbiomes we studied synecological dynamics a microbial mat community (GMMC) vis-à-vis fluctuations in its environment. Spatiotemporally-discrete editions photosynthetic GMMC colonizing travertine mound circum-neutral hot spring cluster served as model-system. In 2010 strong geyser atop discharged mineral-rich water, which nourished continuum from proximal channels (PC) upto slope environment...
Abstract. To explore the potential role of tetrathionate in sedimentary sulfur cycle, population ecology microorganisms capable metabolizing this polythionate was revealed at 15–30 cm resolution along two, ∼3 m long, cores collected from 530 and 580 below sea level, off India's west coast, within oxygen minimum zone (OMZ) Arabian Sea. Metagenome analysis widespread occurrence genes involved formation, oxidation, reduction tetrathionate; high diversity relative abundance were also detected...
Abstract. Marine sedimentation rate and bottom-water O2 concentration control organic carbon remineralization sequestration across continental margins, but whether how they shape microbiome architecture (the ultimate effector of all biogeochemical phenomena) shelf slope sediments is still unclear. Here we reveal distinct structures functions, amidst comparable pore fluid chemistries, along 300 cm sediment horizons underlying the seasonal (shallow coastal; water depth: 31 m) perennial (deep...
The facultatively sulfur-oxidizing chemolithoautotrophic alphaproteobacterium Pseudaminobacter salicylatoxidans KCT001 (MTCC 7265) belongs to the family Phyllobacteriaceae of order Rhizobiales. Analysis its genome offers valuable insight into adaptive specializations and evolution free-living soil bacteria that are phylogenetically closely related symbiotic invasive rhizobacteria.
Abstract Metabolically-active obligate aerobes are unheard-of in tightly-anoxic environments. Present culture-independent and culture-dependent investigations revealed aerobic microbial communities along two, ~3-meter-long sediment-cores underlying the eastern Arabian Sea oxygen minimum zone, where high H 2 S disallows O influx from water-column. While genes for respiration by aa 3 -/ cbb -type cytochrome- c oxidases bd ubiquinol oxidase, oxidation of...
Exploration of the aquatic microbiota several circum-neutral (6.0-8.5 pH) mid-temperature (55-85°C) springs revealed rich diversities phylogenetic relatives mesophilic bacteria, which surpassed diversity truly-thermophilic taxa. To gain insight into potentially-thermophilic adaptations Gram-negative bacteria detected in culture-independent investigations we attempted pure-culture isolation by supplementing enrichment media with 50 μg ml(-1) vancomycin. Surprisingly, this...
The bacterial diseases black leg and soft rot in potatoes cause heavy losses of worldwide. Bacteria within the genus Pectobacteriaceae are causative agents rot. use antibiotics agriculture is heavily regulated no other effective treatment currently exists, but bacteriophages (phages) have shown promise as potential biocontrol agents. In this study we isolated bacteria from potato tubers plant tissue displaying or symptoms collected Danish fields. We then used strains hosts for phage...
ABSTRACT Here, we present the draft genome sequence of Geobacillus thermoleovorans strain N7 (MCC 3175), isolated from Paniphala Hot Spring, West Bengal, India, which contains genes that encode several industrially and medically important thermostable enzymes like neutral protease, xylose isomerase, rhamnogalacturonan acetylesterase, nitrate nitrite reductase, l -asparaginase, glutaminase, RNase P .
This study elucidates the genomic basis of evolution pathogens alongside free-living organisms within family Alcaligenaceae Betaproteobacteria. Towards that end, complete genome sequence sulfur-chemolithoautotroph Tetrathiobacter kashmirensis WT001T was determined and compared with soil isolate Achromobacter xylosoxidans A8 two Bordetella bronchiseptica RB50 Taylorella equigenitalis MCE9. All analyses comprehensively indicated MCE9 genomes were almost subsets WT001T, respectively. In...
Bacteriophages are viral agents that infect and replicate within bacterial cells. Despite the increasing importance of phage ecology, environmental phages-particularly those targeting phyllosphere-associated bacteria-remain underexplored, current genomic databases lack high-quality genome sequences linked to specific environmentally important bacteria, such as ubiquitous sphingomonads. Here, we isolated three novel phages from a Danish wastewater treatment facility. Notably, these among...
ABSTRACT Here, we present the draft genome of Haladaptatus sp. strain R4, a halophilic archaea that produces an orange-pink pigment and is capable growing in wide salinity range. The assembly shows genes for arsenic resistance, siderophore production, trehalose glycine betaine biosynthesis, uptake transporters sodium, potassium, chloride ions.
For a holistic understanding of microbial life’s high-temperature adaptation, it is imperative to explore the biology phylogenetic relatives mesophilic bacteria which get stochastically introduced geographically and geologically diverse hot spring systems by local geodynamic forces. Here, in vitro endurance high heat up extent growth under special (habitat-inspired) conditions was discovered hot-spring-dwelling relative Paracoccus species. Thermal conditioning, extreme oligotrophy, metabolic...
ABSTRACT Here we report the draft genome sequence of Meiothermus taiwanensis strain RP (MCC 2966), isolated from Paniphala hot spring India, which contains genes encoding for enzymes methyl erythritol 4-phosphate (MEP) pathway isoprenoid biosynthesis and carotenoid backbone synthesis.
To understand SARS-CoV-2 microevolution, this study explored the genome-wide frequency, gene-wise distribution, and molecular nature of all point-mutations detected across its 71,703 RNA-genomes deposited in GISAID repository, till 21 August 2020. Globally, nsp1/nsp2/nsp3/ nsp11 orf7a/orf3a/S were most mutation-ridden non-structural structural genes respectively. Phylogeny based on 4,618 spatiotemporally-representative genomes revealed that entities belonging to early lineages are mostly...