A5029586330- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Bioactive Compounds and Antitumor Agents
- Toxin Mechanisms and Immunotoxins
- Epoxy Resin Curing Processes
- Protein Tyrosine Phosphatases
- Fungal Biology and Applications
- Immune Cell Function and Interaction
- Fermentation and Sensory Analysis
- Fluorine in Organic Chemistry
- Cancer, Hypoxia, and Metabolism
- Advanced Cellulose Research Studies
- vaccines and immunoinformatics approaches
- Enzyme Production and Characterization
- Plant responses to water stress
- Immunotherapy and Immune Responses
- Fiber-reinforced polymer composites
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Biochemical and Structural Characterization
- Research on Leishmaniasis Studies
- Malaria Research and Control
- Enzyme-mediated dye degradation
- Antifungal resistance and susceptibility
- Advanced Proteomics Techniques and Applications
BOKU University
2020-2024
Universidade Estadual de Campinas (UNICAMP)
2018-2019
Université de Montréal
2013-2016
Institute for Research in Immunology and Cancer
2013-2016
University Hospital of Lausanne
2010-2011
University of Lausanne
2010
For decades, the global impact of genomic polymorphisms on repertoire peptides presented by major histocompatibility complex (MHC) has remained a matter speculation. Here we present novel approach that enables high-throughput discovery polymorphic MHC class I-associated (MIPs), which play role in allorecognition. On basis comprehensive analyses landscape MIPs eluted from B lymphoblasts two MHC-identical siblings, show 0.5% non-synonymous single nucleotide variations are represented MIP...
Targeting parasite's protein kinase Malaria elimination goals are constantly eroded by the challenge of emerging drug and insecticide resistance. Alam et al. have taken established targets—CLK kinases involved in regulation RNA splicing—and investigated how inhibition enzymes blocks completion its complex life cycle. They identified an inhibitor CLK that was 100-fold less active against most closely related human effective at clearing rodent malaria parasites. Not only does this compound...
Abstract The peptide repertoire presented by classical as well nonclassical MHC class I (MHC I) molecules is altered in the absence of endoplasmic reticulum aminopeptidase associated with Ag processing (ERAAP). To characterize extent these changes, peptides from cells lacking ERAAP were eluted cell surface and analyzed high-throughput mass spectrometry. We found that most wild-type (WT) retained ERAAP. In contrast, a subset “ERAAP-edited” was lost WT cells, ERAAP-deficient unique “unedited”...
Aspergillus fumigatus grows well at neutral and acidic pH in a medium containing protein as the sole nitrogen source by secreting two different sets of proteases. Neutral favors secretion alkaline endoproteases, leucine aminopeptidases (Laps) which are nonspecific monoaminopeptidases, an X-prolyl dipeptidase (DppIV). Acidic environment promotes aspartic endoprotease pepsin family (Pep1) tripeptidyl-peptidases sedolisin (SedB SedD). A novel prolyl peptidase, AfuS28, was found to be secreted...
Abstract The dermatophytes are a group of closely related fungi which responsible for the great majority superficial mycoses in humans and animals. Among various potential virulence factors, their secreted proteolytic activity attracts lot attention. Most dermatophyte‐secreted proteases have so far been isolated vitro neutral or alkaline enzymes. However, inspection recently decoded dermatophyte genomes revealed many other hypothetical proteases, particular acidic similar to those...
The bark of trees contains an interesting mixture bioactive compounds, or so-called extractives. use supercritical carbon dioxide (sc-CO2) eliminates both the need for organic solvents as extractants and danger that solvent traces might compromise purity extracts. Unfortunately, complexity natural variability extracts' composition render any utilization attempts rather challenging. Thus, in order to implement exploitation concepts a meaningful way, appropriate analytical techniques...
The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While purity desired product major importance many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous protease. This enzyme was completely inhibited phenylmethanesulfonyl fluoride (PMSF) but not 1,10-phenanthroline, EDTA, and pepstatin A, suggesting nature serine Its secretion abolished P. strains GS115 KM71 specific mutagenesis...
In an acidic protein medium Aspergillus fumigatus secretes aspartic endoprotease (Pep) as well tripeptidyl-peptidases, a prolyl-peptidase and carboxypeptidases. addition, LC-MS/MS revealed novel glutamic protease, AfuGprA, homologous to niger aspergillopepsin II. The importance of AfuGprA in digestion was evaluated by deletion its encoding gene A. wild-type D141 pep Δ mutant. Either Pep or shown be necessary for fungal growth at low pH. Exoproteolytic activity is therefore not sufficient...
The impact of totally chlorine-free (TCF) bleaching chemicals on the composition and behavior detrimental lipophilic wood extractives in a mixed hardwood sulfite pulp was studied to determine their potential for pitch control without need additional reagents. Toward this end, acetone extracts were analyzed with commonly used gas chromatography (GC) more sophisticated ultraperformance convergence (UPC2). During EO(P)–Z–P bleaching, highest decrease extractive content achieved after ozone (Z),...
The novel guanidine-based ionic liquid [mTBNH][OAc], which is suitable for an environmentally friendly lyocell-like spinning process, exhibits both high natural and thermal stability, superior to that of IONCELL ILs.
In a context where injection of antigen (Ag)-specific T cells probably represents the future leukemia immunotherapy, identification optimal target Ags is crucial. We therefore sought to discover reliable marker for selection most potent Ags. To this end, (1) we immunized mice against 8 individual Ags: 4 minor histocompatibility (miHAs) and leukemia-associated (LAAs) that were overexpressed on leukemic relative normal thymocytes; (2) assessed their ability reject EL4 cells; (3) correlated...
Resistance to drought stress is fundamental plant survival and development. Abscisic acid (ABA) one of the major hormones involved in different types abiotic biotic responses. ABA intracellular signaling has been extensively explored Arabidopsis thaliana occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related kinases). However, role biochemistry SnRK2 crop plants remains underexplored. Considering importance hormone tolerance, here we...
Abstract Resistance to drought stress is fundamental plant survival and development. Abscisic acid (ABA) one of the major hormones involved in different types abiotic biotic responses. ABA intracellular signaling has been extensively explored Arabidopsis thaliana occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related kinases). However, role biochemistry SnRK2 crop plants remains underexplored. Considering importance hormone tolerance,...