A5044061571- Osteoarthritis Treatment and Mechanisms
- Orthopaedic implants and arthroplasty
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Knee injuries and reconstruction techniques
- Trace Elements in Health
- Tissue Engineering and Regenerative Medicine
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Titanium Alloys Microstructure and Properties
- Nanoparticles: synthesis and applications
- Adipokines, Inflammation, and Metabolic Diseases
- Shoulder Injury and Treatment
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Heavy Metal Exposure and Toxicity
- Animal Genetics and Reproduction
- Biomedical Ethics and Regulation
- Cancer Cells and Metastasis
- Geological Formations and Processes Exploration
- Magnesium Alloys: Properties and Applications
AstraZeneca (United Kingdom)
2024-2025
Poznan University of Medical Sciences
2015-2024
Concern Worldwide
2023
Adam Mickiewicz University in Poznań
2019
Hematopoietic progenitor kinase 1 (HPK1) is a negative regulator of the T cell receptor signaling pathway and therefore target interest for immunooncology. Nonselective HPK1 inhibitors may affect other components activation, blunting beneficial impact enhanced activity that results from inhibition itself. Here, we report discovery pyrazine carboxamide their optimization through structure-based drug design to afford highly selective inhibitor, compound 24 (AZ3246). This induces IL-2 secretion...
Accelerated telomere shortening is associated with age-related diseases, including osteoarthritis (OA). We aimed to determine the relative length (TL) in leukocytes and cartilage of patients primary knee OA investigate factors that may affect TL OA. Relative measurements were performed using qPCR 612 individuals (310 undergoing total arthroplasty (TKA) 302 unaffected controls). also analysed 57 310 patients, measuring severely affected less (control) collected from same knee. Cartilage TLs...
Stem cells (SCs) are a promising approach to regenerative medicine, with the potential treat numerous orthopedic disorders, including osteo-degenerative diseases. The development of human-induced pluripotent stem (hiPSCs) has increased SCs for new treatments. However, current methods differentiating hiPSCs into chondrocyte-like suboptimal and better needed. aim present study was assess four different chondrogenic differentiation protocols identify most efficient method generating...
Unlocking novel E3 ligases for use in heterobifunctional PROTAC degraders is of high importance to the pharmaceutical industry. Over-reliance on current suite ligands used recruit could limit potential their application. To address this, potent DCAF15 were optimized using cryo-EM supported, structure-based design improve micromolar starting points. A binder, compound 24, was identified and subsequently conjugated into PROTACs against multiple targets. Following attempts degrading a number...
The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in treatment joint diseases. A number protocols vitro differentiation iPSC chondrocytes regenerative purposes have been recently described. However, order to use these clinics, elimination animal serum and feeder essential. In our study, a strictly defined controllable protocol...
The nanoindentation method was applied to determine the elastic modulus and hardness of knee articular cartilage. Cartilage samples from both high weight bearing (HWB) low (LWB) femoral condyles were collected patients diagnosed with osteoarthritis (OA). mean HWB cartilage 4.46 ± 4.44 MPa in comparison that LWB region (9.81 8.88 MPa, p < 0.001). Similarly, significantly lower tissue (0.317 0.397 MPa) than (0.455 0.434 When adjusted patients’ ages, age group over 70 years (p A...
Multi-walled carbon nanotubes (MWCNTs) serve as nanoparticles due to their size, and for that reason, when in contact with the biological system, they can have toxic effects. One of main mechanisms responsible nanotoxicity is oxidative stress resulting from production intracellular reactive oxygen species (ROS). Therefore, biomarkers are important tools assessing MWCNTs toxicity. The aim this study was evaluate multi-walled male rats. Our animal model studies (diameter ~15-30 nm, length...
Human induced pluripotent stem cells (hiPSCs) play an important role in research regarding regenerative medicine. Particularly, chondrocytes differentiated from hiPSCs seems to be a promising solution for patients suffering osteoarthritis. We decided perform chondrogenesis three-week monolayer culture. Based on transcriptome analysis, hiPSC-derived (ChiPS) demonstrate the gene expression profile of early chondrogenesis. Chondrogenic progenitors obtained by our group are characterized...
Continuous passive motion (CPM) is a frequently used method in the early post-operative rehabilitation of patients after knee surgery. In this study, effectiveness CPM was evaluated primary total arthroplasty during an recovery period.Eighty undergoing were assigned into two groups. The experimental group received and active exercises, while control exercises only. All subjects once before surgery at discharge, terms mean range (AROM), Knee Society Score (KSS), Western Ontario MacMaster...
The study was designed to investigate whether serum concentrations of leptin, resistin and adiponectin in obese normal-weight patients with primary knee osteoarthritis (OA) correlate clinical radiological stages the disease percentage total body fat.Seventy-three OA, divided into groups, were clinically evaluated according Knee Society Score (KSS), radiologically assessed using Kellgren Lawrence scale. fat some anthropometric data also given. Serum measured by Elisa correlated clinical,...
To improve the recovery of damaged cartilage tissue, pluripotent stem cell-based therapies are being intensively explored. A number techniques exist that enable monitoring cell differentiation, including immunofluorescence staining. This simple and fast method enables changes to be observed during differentiation process. Here, two protocols for human embryonic cells into chondrocytes were used (monolayer culture embryoid body formation). Cells labeled markers expressed process at different...
Human induced pluripotent stem cells (hiPSCs) offer promise in regenerative medicine, however more data are required to improve understanding of key aspects the cell differentiation process, including how specific chondrogenic processes affect gene expression profile chondrocyte‑like and relative value markers. The main aims present study were as follows: To determine chondrogenic-like derived from hiPSCs cultured mediums conditioned with HC‑402‑05a or supplemented transforming growth factor...
One of the obstacles to autologous chondrocyte implantation (ACI) is obtaining a large quantity chondrocytes without depletion their properties. The conditioned medium (CM) from different subpopulations stem cells (mesenchymal stromal (MSC) or induced pluripotent (iPSC)) could be gamechanger. MSCs' potential related donor's health and age, which omitted when, as source, iPSCs are used. There lack data regarding use in culture expansion. Thus, we wanted verify whether iPSC-CM beneficial for...