João Luís Garcia

ORCID: 0000-0003-1826-7582
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About
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Research Areas
  • Toxoplasma gondii Research Studies
  • Herpesvirus Infections and Treatments
  • Parasitic Infections and Diagnostics
  • Rabies epidemiology and control
  • T-cell and Retrovirus Studies
  • Coccidia and coccidiosis research
  • Vector-borne infectious diseases
  • Cytomegalovirus and herpesvirus research
  • Viral Infections and Vectors
  • Animal Disease Management and Epidemiology
  • Vector-Borne Animal Diseases
  • Microbial infections and disease research
  • Helminth infection and control
  • Animal Nutrition and Physiology
  • Leptospirosis research and findings
  • Research on Leishmaniasis Studies
  • Sex work and related issues
  • Veterinary medicine and infectious diseases
  • Trypanosoma species research and implications
  • Amoebic Infections and Treatments
  • Heme Oxygenase-1 and Carbon Monoxide
  • Viral gastroenteritis research and epidemiology
  • Bird parasitology and diseases
  • Ocular Surface and Contact Lens
  • Parasite Biology and Host Interactions

Universidade Estadual de Londrina
2016-2025

Universidade de Brasília
2023

National Laboratory for Civil Engineering
2023

Universidade Estadual de Maringá
2019

Moredun Research Institute
2015-2017

Associação de Assistência à Criança Deficiente
2015

University of Veterinary Medicine Vienna
2013

Universidade Norte do Paraná
2013

Unidad Ejecutora Lillo
2012

Universidade Estadual do Centro-Oeste
2004-2007

The aim of the present study was to evaluate re-shedding T. gondii oocysts in cats fed tissue cysts homologous and heterologous strains 12, 24 36 months after first infection. Thirteen were used divided into four groups: G1 (n=2), G2 (n=3), G3 (n=5), G4 (n=3). G1, infected with brain ME49 TgDoveBr8, both genotype II gondii. re-infected twelve VEG strain (genotype III), TgDoveBr1 II). a third time from second infection, initial infection strain. cats' feces evaluated using fecal flotation...

10.1016/j.vetpar.2017.10.021 article EN publisher-specific-oa Veterinary Parasitology 2017-10-31

The concomitant infections of Canine distemper virus (CDV), adenovirus A types 1 (CAdV-1) and 2 (CAdV-2), parvovirus type (CPV-2), Toxoplasma gondii are described in a 43-day-old mixed-breed puppy. Clinically, there were convulsions blindness with spontaneous death; 14 siblings this puppy, born to 10-month-old dam, which was seropositive (titer: 1,024) for T. gondii, also died. Necropsy revealed unilateral corneal edema (blue eye), depletion intestinal lymphoid tissue, non-collapsible lungs,...

10.1177/1040638712471344 article EN Journal of Veterinary Diagnostic Investigation 2013-01-01

O Toxoplasma gondii, agente da toxoplasmose, encontra-se distribuído mundialmente. No presente estudo, determinou-se a prevalência do T. gondii nas espécies suína, bovina, ovina e eqüina oriundas de fazendas município Jaguapitã , através imunofluorescência indireta para detecção anticorpos classe - IgG, considerando-se sororeagentes aqueles títulos maiores ou iguais 1:64. Verificou-se uma 24% em 267 amostras suínos, 25,8% 400 bovinos, 51,8% 228 ovinos 12,1% 173 eqüinos. Com relação às faixas...

10.1590/s0103-84781999000100017 article PT cc-by-nc Ciência Rural 1999-03-01

Abstract Background Concerning the infection of humans by T. gondii, limited efforts have been directed to elucidation role horizontal transmission between hosts. One main routes from animals occurs through ingestion raw or insufficiently cooked meat. However, even though detection gondii in meat constitutes an important short-term measure, control strategies can only be accomplished a deeper understanding epidemiology toxoplasmosis. The present study aimed investigate seroprevalence...

10.1186/1756-3305-6-191 article EN cc-by Parasites & Vectors 2013-06-25

As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement control strategy against Toxoplasma gondii (T. gondii) this species reduce establishment of T. tissue cysts pork, consequently reducing risk parasite entering human food chain. Consumption from raw or undercooked meat one sources infection, with infected pork being high risk. This study incorporates mouse bioassay molecular detection DNA effectiveness vaccination (incomplete S48 strain) its ability...

10.1186/s13567-015-0177-0 article EN cc-by Veterinary Research 2015-04-30

The aim of this study was to evaluate associations between seropositivity for IgG and IgM anti-Toxoplasma gondii antibodies socio-economic environmental variables in pregnant women Londrina, state Paraná, Brazil. We interviewed 492 women, each whom answered an epidemiological questionnaire, collected blood samples measurement anti-T. by chemiluminescence. A confirmatory diagnosis acute infection made avidity test. Titres specific were obtained IFAT. Seropositivity observed 242 (49.2%) and,...

10.1590/s0074-02762009000200036 article EN Memórias do Instituto Oswaldo Cruz 2009-03-01

Bovine hemoplasmas are bacteria found on the erythrocyte surface or free in plasma of cattle. The aim present study was to evaluate occurrence 'Candidatus Mycoplasma haemobos' ('C. M. haemobos') Holstein and Jersey cattle raised Londrina surroundings, northern region State Parana, Southern Brazil. PCR testing directed 16S rRNA gene fragment performed investigate characterize molecular identity 'C. haemobos'. A total 264/433 (60.97%) blood samples were positive by PCR. Further alignment...

10.1590/s1984-29612012000300034 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2012-09-01

The aims of this study were to verify the prevalence Cryptosporidium spp. and Giardia in animal feces drinking water on dairy farms identify a possible relation between exposure factors presence these parasites. Fecal samples from cattle humans collected Paraná, Brazil. Analysis (oo)cysts was performed by modified Ziehl-Neelsen staining centrifugal flotation zinc sulfate. Test-positive subjected nested PCR amplification 18SSU ribosomal RNA gene for identification gp60 subtyping...

10.1371/journal.pone.0175311 article EN cc-by PLoS ONE 2017-04-12

A retrospective immunohistochemical study was designed to investigate the frequency of concomitant traditional infectious disease pathogens in puppies that died suddenly and review aspects associated pathogenesis. Fifteen were evaluated; pathology reports histopathologic slides these animals reviewed determine pattern lesions. The intralesional identification antigens canine (distemper) morbillivirus (CDV), adenovirus-1 -2 (CAdV-1 -2), parvovirus-2 (CPV-2), Toxoplasma gondii, Neospora...

10.1038/s41598-018-31540-0 article EN cc-by Scientific Reports 2018-09-03

Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence E. A. in blood samples collected from 256 domiciled dogs municipality Jataizinho, located north region State Parana, Brazil, PCR assay. The occurrence was 16.4% (42/256) 19.4% (49/256), respectively; while...

10.1590/s1984-29612012005000009 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2012-12-01

Considering that the current immunoassays are not able to distinguish infective forms cause Toxoplasma gondii infection, present study was carried out evaluate reactivity of two recombinant proteins (CCp5A and OWP1) from oocyst/sporozoite, in order differentiate infections occurring by ingestion oocysts or tissue cysts. The assessed against panels serum samples animals (chickens, pigs mice) were naturally experimentally infected different stages parasite. Also, we tested sera humans who have...

10.3389/fmicb.2015.01305 article EN cc-by Frontiers in Microbiology 2015-11-24

Eared doves (Zenaida auriculata), which are common in urban, rural and wild areas many regions of Brazil, frequently prey for domestic cats. Therefore Toxoplasma gondii isolates obtained from may reflect greater environmental diversity than those other hosts. The aim the present study was to evaluate T. seroprevalence, isolate genotype strains Z. auriculata. Serum tissue samples were collected 206 use modified agglutination test (MAT) mouse bioassay. prevalence antibodies 22.3% (46/206),...

10.1590/s1984-29612014073 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2014-12-01

Abstract: Toxoplasma gondii is an intracellular protozoan parasite that can infect many warm-blooded animal species and humans. Despite substantial knowledge of the biology, epidemiology, host-pathogen interactions T. , there are still very few effective control strategies to prevent oocyst shedding in cats, tissue cysts livestock for consumption, infection disease This article reviews current progress targets vaccination against . Keywords: toxoplasmosis, vaccination, animals, immune...

10.2147/vdt.s57474 article EN Vaccine Development and Therapy 2014-04-01

To evaluate the Western blotting method for detection of IgG anti-Toxoplasma gondii (T. gondii) (IgG-WB) in serum children with suspected congenital toxoplasmosis.We accompanied 47 mothers acquired toxoplasmosis pregnancy and their children, between June 2011 2014. The IgG-WB was done house test considered positive if child had antibodies that recognized at least one band on blots different from mother's or greater intensity than corresponding maternal band, during first three months life.15...

10.1016/j.jped.2016.02.014 article EN cc-by-nc-nd Jornal de Pediatria 2016-08-06

This study aimed to detect parasites from Leishmania genus, determine the prevalence of anti-Leishmania spp. antibodies, identify circulating species parasite, and epidemiological variables associated with infection in rats caught urban area Londrina, Paraná, Brazil. Animal capture was carried out May December 2006, serological molecular methods were performed. DNA extracted total blood, nested-PCR, targeting SSu rRNA performed triplicate. The positive samples sequenced twice by Sanger...

10.1590/s1984-29612017001 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2017-02-06
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