A5069801508- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Nosocomial Infections in ICU
- Respiratory Support and Mechanisms
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Gun Ownership and Violence Research
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Pediatric health and respiratory diseases
- Injury Epidemiology and Prevention
- Infectious Encephalopathies and Encephalitis
- Gut microbiota and health
- Respiratory viral infections research
- Suicide and Self-Harm Studies
- Disaster Response and Management
- COVID-19 diagnosis using AI
- COVID-19 and healthcare impacts
- Healthcare Decision-Making and Restraints
University of Pennsylvania
2020-2021
Museum of Heilongjiang Province
2021
Office of Infectious Diseases
2020
New York University
2015-2019
Structural DNA nanotechnology combines branched junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals structural is construct three-dimensional (3D) crystalline lattices. Here we present a new motif and strategy that has led assembly 3D lattice. We have determined X-ray crystal structures two related constructs 3.1 Å resolution using bromine-derivatized crystals. The used employs five-nucleotide repeating sequence weaves through...
DNA tensegrity triangles self-assemble into rhombohedral three-dimensional crystals via sticky ended cohesion. Crystals containing two-nucleotide (nt) ends (GA:TC) have been reported previously, and those diffracted to 4.9 Å at beamline NSLS-I-X25. Here, we analyze the effect of varying end lengths sequences as well impact 5′- 3′-phosphates on crystal formation resolution. Tensegrity triangle motifs having 1-, 2-, 3-, 4-nt all form crystals. X-ray diffraction data from same reveal that...
Cross-linked crystals: triplex-forming oligonucleotides can direct cross-linking reactions within or between tiles of a DNA crystal, improving their thermal stability.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse-transcription polymerase chain reaction (RT-PCR) cycle threshold (Ct) has been used to estimate quantitative viral load, with the goal of targeting isolation precautions for individuals disease 2019 (COVID-19) and guiding public health interventions. However, variability in specimen quality can alter Ct values obtained from SARS-CoV-2 clinical assays. We sought define how variable nasopharyngeal (NP) swab impacts test...
The construction of DNA nanostructures from branched motifs, or tiles, typically relies on the use sticky-ended cohesion, owing to specificity and programmability sequences. stability such constructs when unligated is restricted a specific range temperatures, disruption base pairing at elevated temperatures. Paranemic (PX) cohesion was developed as an alternative sticky ends for large topologically closed species that could be purified reliably denaturing gels. However, PX also limited...
Catenation is the process by which cyclic strands are combined like links of a chain, whereas knotting changes linking properties single strand. In cell, topoisomerases catalyzing strand passage operations enable and catenation DNA so that single- or double-stranded segments can be passed through each other. Here, we use system closed structures involving paranemic motif, called PX-DNA, to bind double together. These PX-cohesive molecules contain complementary loops whose Escherichia coli...
Much has been reported on the clinical course of severe COVID-19, but less is known about natural history and sequalae mildly symptomatic cases prospects reinfection or recurrence symptoms. We report a case patient with PCR-confirmed COVID-19 who, after being symptom-free for 2 weeks, redeveloped symptoms was found to be PCR-positive again >4 weeks from original testing. Surprisingly, IgG IgM antibody testing negative months reinfection. Although no performed between two bouts, this raises...
Abstract Background The SARS-CoV-2 reverse-transcription polymerase chain reaction (RT-PCR) cycle of threshold (Ct) has been used to estimate quantitative viral load, with the goal targeting isolation precautions for individuals COVID-19 and guiding public health interventions. However, variability in specimen quality can alter Ct values obtained from clinical assays. We sought define how variable nasopharyngeal (NP) swab impacts test sensitivity. Methods performed amplification a human gene...
Tracheal intubation and mechanical ventilation provide essential support for patients with respiratory failure, but the course of may be complicated by adverse ventilator-associated events (VAEs), which or not associated infection. We sought to understand how frequency subglottic suction, an indicator quantity sputum produced ventilated patients, relates onset all VAEs infection-associated VAEs.
Ventilator-associated lower respiratory tract infection (VA-LRTI) is common among critically ill patients and has been associated with increased morbidity mortality. In acute critical illness, microbiome disruption indices (MDIs) have shown to predict risk for VA-LRTI, but their utility beyond the first days of illness unknown. We sought characterize how MDIs previously VA-LRTI at initiation mechanical ventilation change prolonged ventilation, if they remain risk.
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Abstract Background Patients admitted to long-term acute care hospital (LTACH) for ventilator weaning are at high risk ventilator-associated pneumonia, which may contribute adverse events (VAE). Staphylococcus aureus (Sa) is a common cause of VAP. We sought evaluate the impact respiratory Sa colonization and bacterial community dominance on subsequent VAP VAE during care. Methods enrolled 83 subjects dependent mechanical ventilation LTACH admission, collected endotracheal aspirates,...
Abstract Background Although SARS-CoV-2 predominantly targets the respiratory system, it has also been associated with vascular complications including stroke. Identifying COVID-19 patients at elevated risk for stroke can help inform target anticoagulation strategies. We sought to understand how symptoms and laboratory markers presentation relate risk. Methods enrolled a cohort of 1324 subjects who were hospitalized across six PennMedicine hospitals between April August 2020 performed...