A5017604643- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Connexins and lens biology
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Research on Leishmaniasis Studies
- Diamond and Carbon-based Materials Research
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Immunotherapy and Immune Responses
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Protein purification and stability
- Trypanosoma species research and implications
- Diet and metabolism studies
- Protein Structure and Dynamics
- Natural product bioactivities and synthesis
Tripura University
2022-2025
Dr. D.Y. Patil Vidyapeeth, Pune
2017-2025
Punjabi University
2025
Government Medical College and Hospital
2021
Raiganj University
2021
National Institute of Technology Durgapur
2020
KPC Medical College and Hospital
2019
Savitribai Phule Pune University
2009-2018
Vancouver General Hospital
2014
Medical College and Hospital, Kolkata
2013
We have cloned the cDNA of heme-regulated eIF-2 alpha kinase (HRI) rabbit reticulocytes. In vitro translation mRNA transcribed from HRI yields a 90-kDa polypeptide that exhibits activity and is recognized by monoclonal antibody directed against authentic HRI. The open reading frame sequence contains all 11 catalytic domains protein kinases with consensus sequences protein-serine/threonine in conserved VI VIII. also an insert approximately 140 amino acids between V VI. coding has extensive...
Dioscorea bulbifera is a potent medicinal plant used in both Indian and Chinese traditional medicine owing to its rich phytochemical diversity. Herein, we report the rapid synthesis of novel Au core Ag shell nanoparticles by D. tuber extract (DBTE). NPs was completed within 5 h showing prominent peak at 540 nm. HRTEM analysis revealed 9 nm inner elemental gold covered silver giving total particle diameter upto 15 were comprised 57.34 ± 1.01% 42.66 0.97% mass. showed highest biofilm...
Abstract Cellular mRNAs are predominantly translated in a cap-dependent manner. However, some viral and subset of cellular initiate their translation cap-independent This requires presence structured RNA element, known as, Internal Ribosome Entry Site (IRES) 5′ untranslated regions (UTRs). Experimental demonstration IRES UTR remains challenging task. Computational prediction merely based on sequence structure conservation is also difficult, particularly for IRES. A web server, IRESPred...
ABSTRACT Prosomes, ubiquitous ribonucleoprotein (RNP) particles of defined biochemical and morphological structure, first isolated as a subcomplex the repressed globin mRNP in avian mouse erythroblasts, were also found cytoplasm other vertebrates associated with mRNAs. Here we show that prosomes are present cell nucleus and, furthermore, cytolocalization specific prosomal peptides is function differentiation. Four monoclonal antibodies, raised against duck proteins, p27K, p28K, p29K p31K (K...
ABSTRACT The prosomes, biochemically well characterized small RNA-protein complexes, found associated with mRNA in all eukaryotic cells tested, have been identified as maternal components sea urchin and chick embryos. In this study, we investigated their presence cytolocalization the oocytes embryos of Pleurodeles waltl by immunoblot analysis immunofluorescence, using monoclonal antibodies prepared against duck prosome proteins. Of four anti-bodies three recognized corresponding antigens...
Innate immunity in animals including humans encompasses the complement system, which is considered an important host defense mechanism against Aspergillus fumigatus, one of most ubiquitous opportunistic human fungal pathogens. Previously, it has been shown that alkaline protease Alp1p secreted from A. fumigatus mycelia degrades components C3, C4, and C5. However, remains unclear how spores (i.e. conidia) defend themselves activities system immediately after inhalation into lung. Here, we...
Inhibition of protein synthesis in rabbit reticulocyte lysates occurs response to a variety conditions including heme deficiency, addition oxidants, and heat stress.The inhibition translation is due the activation heme-regulated kinase (HRI) which specifically phosphorylates a-subunit eukaryotic initiation factor eIF-2.In this report, immunoadsorption with monoclonal antibodies (mAbs) Western blot analysis were used investigate interaction HRI, 90-kDa shock (hsp 90), hsp 70, EC1 antigen...
A recent surge in finding new candidate vaccines and potential antivirals to tackle atypical pneumonia triggered by the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) needs unexplored approaches solving this global pandemic. The homotrimeric transmembrane spike (S) glycoprotein of coronaviruses which facilitates virus entry into host cells is covered with N-linked glycans having oligomannose complex sugars. These provide a unique opportunity for their targeting via...
ADVERTISEMENT RETURN TO ISSUEPREVArticleTissue distribution and immunoreactivity of heme-regulated eIF-2.alpha. kinase determined by monoclonal antibodiesJayanta K. Pal, Jane Chen, Irving M. LondonCite this: Biochemistry 1991, 30, 9, 2555–2562Publication Date (Print):March 1, 1991Publication History Published online1 May 2002Published inissue 1 March 1991https://pubs.acs.org/doi/10.1021/bi00223a037https://doi.org/10.1021/bi00223a037research-articleACS PublicationsRequest reuse...
Tumor heterogeneity is influenced by various factors including genetic, epigenetic and axis of metabolic-epigenomic regulation. In recent years, reprogramming has been considered as one the many tumor hallmarks it appears to be driven both microenvironment macroenvironment diet, microbiota environmental pressures. Epigenetically, histone lysine residues are altered post-translational modifications (PTMs) such acetylation, acylation, methylation lactylation. Furthermore, lactylation suggested...
ABSTRACT The advent of the Internet Things (IoT) has transformed concept smart home automation, thereby allowing users to remotely interact with their houses and control appliances for resource efficiency. This technological development significantly improved convenience, safety, overall lifestyles homeowners. impact automation systems (SHAS) extends beyond individual households, positively influencing global economy in various aspects. While research proposed solutions wireless monitoring...
We have purified the heme-regulated eukaryotic initiation factor 2 alpha subunit (eIF-2 alpha) kinase (HRI) from rabbit reticulocytes for amino acid microsequencing. This is a single 92-kDa polypeptide and migrates in perfect alignment with 32P-labeled HRI on SDS/PAGE. Its functions of binding ATP autophosphorylation eIF-2 phosphorylation are inhibited by hemin. The sequences three tryptic peptides been obtained. A search data base National Biomedical Research Foundation reveals that these...