A5021541206- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Corporate Governance and Management
- Digital Innovation in Industries
- Ion channel regulation and function
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Alzheimer's disease research and treatments
- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- thermodynamics and calorimetric analyses
- Nicotinic Acetylcholine Receptors Study
- Neuroendocrine regulation and behavior
- Zebrafish Biomedical Research Applications
- Cardiac electrophysiology and arrhythmias
- Muscle Physiology and Disorders
- Physics and Engineering Research Articles
- Stress Responses and Cortisol
- Gaze Tracking and Assistive Technology
- Corporate Management and Leadership
Heidelberg University
2015-2025
University Hospital Heidelberg
2011-2025
Czech Academy of Sciences, Institute of Physiology
2010-2024
Heidelberg University
2006-2021
Hippocampal theta (5-10 Hz) and gamma (35-85 oscillations depend on an inhibitory network of GABAergic interneurons. However, the lack methods for direct cell-type-specific interference with inhibition has prevented better insights that help link synaptic cellular properties function. Here, we generated genetically modified mice (PV-Deltagamma(2)) in which was ablated parvalbumin-positive (PV+) local field potential unit recordings CA1 area freely behaving revealed rhythm strongly reduced...
Neurons form transiently stable assemblies that may underlie cognitive functions, including memory formation. In most brain regions, coherent activity is organized by network oscillations involve sparse firing within a well-defined minority of cells. Despite extensive work on the underlying cellular mechanisms, fundamental question remains unsolved: how are participating neurons distinguished from majority nonparticipators? We used physiological and modeling techniques to analyze neuronal in...
Abstract Complex experimental protocols often require multi-modal data acquisition with precisely aligned timing, as well state- and behavior-dependent interventions. Tailored solutions are mostly restricted to individual setups lack flexibility interoperability. We present an open-source, Linux-based integrated software solution, called ‘Syntalos’, for simultaneous synchronization of from arbitrary number sources, including multi-channel electrophysiological recordings different live...
Information processing in neuronal networks involves the recruitment of selected neurons into coordinated spatiotemporal activity patterns. This sparse activation results from widespread synaptic inhibition conjunction with neuron-specific excitation. We report selective hippocampal pyramidal cells patterned network activity. During ripple oscillations awake mice, spiking is much more likely which axon originates a basal dendrite rather than soma. High-resolution recordings vitro and...
Long-term memories are believed to be encoded by unique transcriptional signatures in the brain. The expression of immediate early genes (IEG) promotes structural and molecular changes required for memory consolidation. Recent evidence has shown that brain is equipped with mechanisms not only promote, but actively constrict formation. However, it remains unknown whether IEG may play a role suppression. Here we uncovered novel function neuronal PAS domain protein 4 (Npas4), as an inducible...
We use second harmonic generation (SHG) imaging to study and quantify a strong intrinsic SHG signal in skeletal muscle fiber preparations single isolated myofibrils. The follows the striation pattern of cells is positioned at sarcomeric location myosin filaments. Interestingly, enhanced region where heads are located on As reflects subcellular structure an accurate way, can be used for noninvasive high resolution structural without exogenous labels living cells. This may very important...
Abstract The NMDA receptor-mediated Ca 2+ signaling during simultaneous pre- and postsynaptic activity is critically involved in synaptic plasticity thus has a key role the nervous system. In GRIN2 -variant patients alterations of this coincidence detection provoked complex clinical phenotypes, ranging from reduced muscle strength to epileptic seizures intellectual disability. By using our gene-targeted mouse line (Grin2a N615S ) , we show that voltage-independent glutamate-gated...
Cognitive functions go along with complex patterns of distributed activity in neuronal networks, thereby forming assemblies selected neurons. To support memory processes, such have to be stabilized and reactivated a highly reproducible way. The rodent hippocampus provides well studied model system for network mechanisms underlying spatial formation. Assemblies place-encoding cells are repeatedly activated during sleep-associated states called sharp wave–ripple complexes (SPW-Rs). Behavioral...
Abstract Network oscillations bind neurons into transient assemblies with coherent activity, enabling temporal coding. In the mammalian hippocampus, spatial relationships are represented by sequences of action potentials place cells. Such patterns established during memory acquisition and re‐played sharp wave‐ripple complexes in CA1 subsequent sleep episodes. These events originate CA3 travel towards downstream cortical areas. It is unclear, however, whether specific ripple‐associated firing...
Acute cerebral ischemia and chronic neurovascular diseases share various common mechanisms with neurodegenerative diseases, such as disturbed cellular calcium energy homeostasis accumulation of toxic metabolites. A link between these conditions may be constituted by amyloid precursor protein (APP), which plays a pivotal role in the pathogenesis Alzheimer′s disease, but has also been associated response to acute hypoxia regulation homeostasis. We therefore studied hypoxia-induced loss...
We recently reported expression of hematopoietic growth factor GM-CSF and its receptor (GM-CSFR) in CNS neurons. Here we evaluated this system learning memory formation using deficient mice. In complementation, signalling was manipulated specifically adult murine hippocampus by adeno-associated virus (AAV)-mediated GM-CSFR alpha overexpression or knock-down. ablation caused various amygdala-dependent deficits spatial fear while rendering intact basic parameters like motor function, inherent...
Abstract Epileptic seizures can induce pathological processes of plasticity in the brain that tend to promote generation further seizures. However, immediate impact epileptic on cellular excitability remains poorly understood. In order unravel such early mechanisms epilepsy‐induced plasticity, we studied synaptic transmission before and shortly after three ictal discharges induced by transient elevation extracellular K + mouse hippocampal slices. Discharges were initiated CA3 region...
Cortical information processing comprises various activity states emerging from timed synaptic excitation and inhibition. However, the underlying energy metabolism is widely unknown. We determined cerebral metabolic rate of oxygen (CMRO 2 ) along a tissue depth <0.3 mm in hippocampal CA3 region during network activities, including gamma oscillations sharp wave-ripples that occur wakefulness sleep. These physiological associate with sensory perception memory formation, critically depend on...
The entorhinal cortex (EC) is a critical component of the medial temporal lobe (MTL) memory system. Local networks within MTL express variety state‐dependent network oscillations that are believed to organize neuronal activity during formation. peculiar pattern sharp wave‐ripple complexes (SPW‐R) entrains neurons by very fast oscillation at ∼200 Hz in hippocampal areas CA3 and CA1 then propagates through “output loop” into EC. precise mechanisms SPW‐R propagation resulting cellular input...
L-type calcium currents (iCa) were recorded using the two-microelectrode voltage-clamp technique in single short toe muscle fibres of three different mouse strains: (i) C57/SV129 wild-type mice (wt); (ii) mdx (an animal model for Duchenne muscular dystrophy; and (iii) transgenically engineered mini-dystrophin (MinD)-expressing mice. The activation inactivation properties iCa examined 2- to 18-month-old animals. Ca2+ current densities at 0 mV increased with age, but always significantly...
The mammalian hippocampus expresses several types of network oscillations which entrain neurons into transiently stable assemblies. These groups co-active are believed to support the formation, consolidation and recall context-dependent memories. Formation new assemblies occurs during theta- gamma-oscillations under conditions high cholinergic activity. Memory is linked sharp wave-ripple (SPW-R) decreased tone. We hypothesized that increased tone supports plastic changes while low favors...
The physiological function of amyloid precursor protein (APP) and its two homologues APP-like 1 (APLP1) 2 (APLP2) is largely unknown. Previous work suggests that lack APP or APLP2 impairs synaptic plasticity spatial learning. There is, however, almost no data on the role APLP at network level which forms a critical interface between cellular functions behavior. We have therefore investigated memory-related in hippocampal slices from three lines transgenic mice: APPsα-KI (mice expressing...
We present ADINA, an automated pipeline for analyzing and identifying neuronal activity from calcium imaging data to investigate patterns. This entails the detection classification of cell centroids transients (events) that reappeared during different periods as memory consolidation. Specifically, implements a sparse dictionary learning infer most relevant Ca <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2+</sup> patterns, image segmentation...
The mammalian hippocampus expresses highly organized patterns of neuronal activity which form a correlate spatial memories. These memory-encoding ensembles on top different network oscillations entrain neurons in state- and experience-dependent manner. mechanisms underlying activation, timing selection participating are incompletely understood. Here we studied the synaptic one prominent pattern called sharp wave-ripple complexes (SPW-R) involved memory consolidation during sleep. We recorded...