A5087656467- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Supramolecular Self-Assembly in Materials
- Platelet Disorders and Treatments
- Chemical Synthesis and Analysis
- Mass Spectrometry Techniques and Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Photosynthetic Processes and Mechanisms
- NMR spectroscopy and applications
- Electron Spin Resonance Studies
- Molecular spectroscopy and chirality
- Prion Diseases and Protein Misfolding
- Molecular Sensors and Ion Detection
- Monoclonal and Polyclonal Antibodies Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Advanced Glycation End Products research
- Neuropeptides and Animal Physiology
Stony Brook University
2016-2025
SUNY Old Westbury
2022
Boston University
1996-2020
Osaka University
2001-2009
Rockefeller University
1997-2009
Weizmann Institute of Science
2004-2009
Protein Research Foundation
2007-2009
Joint Center for Structural Genomics
2009
UCLouvain
2006-2009
Yale University
1993-2007
The Hartmann-Hahn matching profile in CP-MAS NMR shows a strong mismatch dependence if the MAS frequency is on order of dipolar couplings sample. Under these conditions, breaks down into series narrow bands separated by spinning speed, and it becomes difficult to establish maintain an efficient condition. Variable-amplitude CP (VACP), as introduced previously (Peersen et al., J. Magn. Reson. A104, 334, 1993), has been proven be effective for restoring flat profiles at high speeds. Here,...
HIV-1 entry into CD4 + cells requires the sequential interactions of viral envelope glycoproteins with and a coreceptor such as chemokine receptors CCR5 CXCR4. A plausible approach to blocking this process is use small molecule antagonists function. One inhibitor has been described for CCR5: TAK-779 molecule. To facilitate further development inhibitors antiviral drugs, we have explored how acts prevent infection, mapped its site interaction CCR5. We find that inhibits replication at...
The nature and distribution of amino acids in the helix interfaces four polytopic membrane proteins (cytochrome c oxidase, bacteriorhodopsin, photosynthetic reaction center Rhodobacter sphaeroides, potassium channel Streptomyces lividans) are studied to address role glycine transmembrane packing. In contrast soluble where is a noted breaker, backbone dihedral angles helices largely fall standard alpha-helical region Ramachandran plot. An analysis packing reveals that residues these...
Helix packing is important in the folding, stability, and association of membrane proteins. Packing analysis helical portions 7 integral proteins 37 soluble show that helices have higher values (0.431) than (0.405). The highest originate from small hydrophobic (G A) hydroxyl-containing (S T) amino acids, whereas large aromatic residues values. for are found transmembrane helix–helix interfaces. Glycine alanine occurrence among buried acids proteins, leucine most common residue These...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolid-state carbon-13 NMR detection of a perturbed 6-s-trans chromophore in bacteriorhodopsinG. S. Harbison, O. Smith, J. A. Pardoen, M. L. Courtin, Lugtenburg, Herzfeld, R. Mathies, and G. GriffinCite this: Biochemistry 1985, 24, 6955–6962Publication Date (Print):November 19, 1985Publication History Published online1 May 2002Published inissue 19 November...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVibrational analysis of the all-trans-retinal chromophore in light-adapted bacteriorhodopsinSteven O. Smith, Mark S. Braiman, Anne B. Myers, Johannes A. Pardoen, Jacques M. L. Courtin, Chris Winkel, Johan Lugtenburg, and Richard MathiesCite this: J. Am. Chem. Soc. 1987, 109, 10, 3108–3125Publication Date (Print):May 1, 1987Publication History Published online1 May 2002Published inissue 1...
13C NMR spectra of lyophilized dark-adapted [14-13C]retinyl-labeled bacteriorhodopsin show a large anomalous upfield shift for the 13C-14 resonance assigned to 13-cis isomer, relative both all-trans isomer and model compounds. We attribute this so-called gamma effect, which results from steric interaction between C-14 retinal proton protons on epsilon CH2 lysine. As consequence observation, we infer that is composed mixture all-trans, 15-anti (trans or E) 13-cis, 15-syn (cis Z) isomers....
Several protein conformational disorders (Parkinson and prion diseases) are linked to aberrant folding of proteins into prefibrillar oligomers amyloid fibrils. Although more toxic than their fibrillar counterparts, it is difficult decouple the origin dissimilar toxicity because fibrils differ both in terms structure size. Here we report characterization two 42-residue β (Aβ42) peptide associated with Alzheimer disease that possess similar size toxicity. We find Aβ42 spontaneously forms at Aβ...
Amyloid fibrils associated with Alzheimer's disease and a wide range of other neurodegenerative diseases have cross β-sheet structure, where main chain hydrogen bonding occurs between β-strands in the direction fibril axis. The surface has pronounced ridges grooves when individual parallel orientation amino acids are in-register one another. Here we show that Aβ amyloid fibrils, Met35 packs against Gly33 C-terminus Aβ40 Gly37 Aβ42. These packing interactions suggest protofilament subunits...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChromophore structure in bacteriorhodopsin's O640 photointermediateSteven O. Smith, Johannes A. Pardoen, Patrick P. J. Mulder, Bo. Curry, Johan Lugtenburg, and Richard MathiesCite this: Biochemistry 1983, 22, 26, 6141–6148Publication Date (Print):December 1, 1983Publication History Published online1 May 2002Published inissue 1 December 1983https://pubs.acs.org/doi/10.1021/bi00295a016https://doi.org/10.1021/bi00295a016research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVibrational analysis of the 13-cis-retinal chromophore in dark-adapted bacteriorhodopsinSteven O. Smith, Johannes A. Pardoen, Johan. Lugtenburg, and Richard MathiesCite this: J. Phys. Chem. 1987, 91, 4, 804–819Publication Date (Print):February 1, 1987Publication History Published online1 May 2002Published inissue 1 February 1987https://pubs.acs.org/doi/10.1021/j100288a011https://doi.org/10.1021/j100288a011research-articleACS PublicationsRequest...
Resonance Raman spectra of the BR 568 , 548 K 625 and L 550 intermediates bacteriorhodopsin photocycle have been obtained in 1 H 2 O by using native purple membrane as well regenerated with 14,15- 13 C 12,14- isotopic derivatives retinal. These were selected to determine contribution 14 —C 15 stretch normal modes 1100- 1400-cm -1 fingerprint region characterize coupling NH rock. Normal mode calculations demonstrate that when retinal Schiff base is C[unk]N cis configuration rock are strongly...
The hydrophobic transmembrane domain of glycophorin A contains a sequence motif that mediates dimerization in membrane environments. Long-range interhelical distance measurements using magic angle spinning NMR spectroscopy provide high-resolution structural constraints on the packing dimer interface bilayers. We show direct contacts occur between glycine residues at positions 79 and 83 sequence. Additional Ile76 Gly79 Val80 Gly83 restrict rotational orientation crossing interacting helices....