A5065425448- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Renal and related cancers
- Bacterial biofilms and quorum sensing
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Antimicrobial Peptides and Activities
- Plant responses to water stress
- Genetic and phenotypic traits in livestock
- Biosensors and Analytical Detection
- Antimicrobial agents and applications
- Genetics, Bioinformatics, and Biomedical Research
- Reproductive Health and Technologies
- Caveolin-1 and cellular processes
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Reproductive Biology and Fertility
University of Münster
2018-2023
University Hospital Münster
2018-2023
Abstract Background The routine genetic analysis for diagnosing male infertility has not changed over the last twenty years, and currently available tests can only determine etiology of 4% unselected infertile patients. Thus, to create new diagnostic assays, we must better understand molecular mechanisms infertility. Although next‐generation sequencing allows simultaneous hundreds genes discovery novel candidates related infertility, so far a few gene have enough sound evidence support...
Mutations affecting the germline can result in infertility or generation of germ cell tumors (GCT), highlighting need to identify and characterize genes controlling development. The RNA-binding protein E3 ubiquitin ligase TRIM71 is essential for embryogenesis, its expression has been reported GCT adult mouse testes. To investigate role mammalian embryonic development, we generated a germline-specific conditional Trim71 knockout (cKO) using early primordial (PGC) marker Nanos3 as...
Significance Statement Nuclear exclusion of the cotranscription factor YAP, which is a consequence activation Hippo signaling pathway, leads to FSGS and podocyte apoptosis. Ajuba proteins play an important role in glomerular filtration barrier by keeping pathway inactive. In nephrocytes from Drosophila melanogaster , well-established model system for research, ensure slit diaphragm (SD) formation function. mislocalization proteins, decreased SD formation, rearrangement actin cytoskeleton,...
Abstract STUDY QUESTION Is the vertebrate protein Dead end (DND1) a causative factor for human infertility and can novel in vivo assays zebrafish help evaluating this? SUMMARY ANSWER Combining patient genetic data with functional reveals possible role DND1 male fertility. WHAT IS KNOWN ALREADY About 7% of population is affected by but linking specific gene variants to disease challenging. The function was shown be critical germ cell development several model organisms reliable cost-effective...
New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an Escherichia coli quorum sensing biosensor reporter strain and selected a non-toxic chitosan evaluate its (QS) inhibition activity on bacterial aggregation. To this end, varying degree acetylation (DA) (12 69%) molecular weight (Mw) (29 288 kDa) were studied. Only low DA (~12%) inhibited growth, regardless their Mw. A medium polymerization (named MDP) DA30,...
New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an E. coli quorum sensing biosensor reporter strain, and selected a non-toxic chitosan evaluate its QS inhibition activity on bacterial aggregation. To this end, varying DA (12 69%) Mw (29 288 KDa) were studied. Only low (~12%) inhibited growth, regardless Mw. Chitosan MDP DA30 (DA 42% 115 kDa) was for further SEM imaging studies. exhibited in inverse...
Abstract Mutations affecting the germline can result in infertility or generation of germ cell tumors (GCT), highlighting need to identify and characterize genes controlling complex molecular network orchestrating development. TRIM71 is a stem cell-specific factor essential for embryogenesis, its expression has been reported GCT adult mouse testes. To investigate role mammalian embryonic development, we generated germline-specific conditional Trim71 knockout (cKO) using early primordial...