A5058652773- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Catalysis for Biomass Conversion
- Rural Development and Agriculture
- Plant Pathogens and Fungal Diseases
- Coffee research and impacts
- Diet, Metabolism, and Disease
- Mycorrhizal Fungi and Plant Interactions
- Agricultural and Food Sciences
- Food composition and properties
- Plant-Microbe Interactions and Immunity
- Phytoestrogen effects and research
- Biodiesel Production and Applications
- Polysaccharides and Plant Cell Walls
- Enzyme-mediated dye degradation
- Botanical Research and Applications
Federal University of São João del-Rei
2018-2024
Universidade Federal de Viçosa
2008-2021
Westerdijk Fungal Biodiversity Institute
2019
Utrecht University
2019
Development of efficient methods for production renewable fuels from lignocellulosic biomass is necessary to maximize yields and reduce operating costs. One the main challenges industrial application conversion process high costs cellulolytic enzymes. Recycling enzymes may present a potential solution alleviate this problem. In study associated with insoluble fraction were recycled after enzymatic hydrolysis pretreated sugarcane bagasse, utilizing different processing conditions, enzyme...
Blending of the enzyme extracts produced by different fungi can result in favorable synergetic enhancement blend with regards to main cellulase activities, as well inclusion accessory enzymes that may not be abundant predominantly producing fungi. The Chrysoporthe cubensis:Penicillium pinophilum 50:50 (v/v) herein presented good synergy, especially for FPase and endoglucanase activities which were 76% 48% greater than theoretical, respectively. This was applied sugarcane bagasse previously...
An intracellular β-glucosidase from Debaryomyceshansenii UFV-1 was produced in an YP medium with cellobiose as the carbon source. This enzyme purified, characterised and presented a Mr of 65.15kDa. Yeast cells containing were immobilised calcium alginate. The free optima values pH temperature 6.0 45°C 5.5 50°C, respectively. maintained 62% 47% its original activity after 90days at 4°C 15days room temperature, immobilisation process resulted higher thermostability 45 50°C. Soy molasses...
Two intracellular invertases from Aspergillus terreus were purified and characterized. Invertase 1 has been shown maximal activity at pH 3.0 optimum temperature 60°C, while invertase 2 presented 4.6 55°C. Km of for sucrose 9.8 6.2 mM, respectively. showed high thermostability presenting half-life 44, 14.9, hr 40, 55, Gel filtration SDS-PAGE analysis revealed that a dimeric structure composed by two monomers 32 kDa. Fructose was competitive inhibitor Ki 61.5 mM. Besides hydrolytic activity,...
Myceliophthora thermophila is a thermophilic ascomycete fungus that used as producer of enzyme cocktails in plant biomass saccharification. Further development this species an industrial factory requires detailed understanding its regulatory systems driving the production biomass-degrading enzymes. In study, we analyzed function MtXlr1, ortholog (hemi-)cellulolytic regulator XlnR first identified another industrially relevant fungus, Aspergillus niger. The Mtxlr1 gene was deleted and...
Exoinulinase (β-d-fructan fructohydrolase, EC 3.2.1.80) secreted by Aspergillus terreus CCT4083 was obtained using sugar cane bagasse, an agroindustrial residue, as a carbon source. It further purified from the supernatant culture in rapid procedure. The enzyme presented 57 kDa on SDS-PAGE and 56 gel filtration chromatography. Inulin hydrolyzed enzyme, yielding d-fructose main product. This showed maximum activity at pH 4.0 60 °C maintained more than 90 75% of its original 40 50 °C,...
Two α -galactosidase (P1 and P2) one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ºC were partially purified by gel filtration hydrophobic interaction chromatographies. Optimum pH temperatures P1, P2 preparations 4.5-5.0, 5.5 4.0 60, 55 65ºC, respectively. The K M app Ï� -nitrophenyl-α -D-galactopyranoside 1.32 mM 0.72 P1 P2, respectively, while value invertase, using sacarose as a substrate was 15.66 mM. Enzyme maintained their activities...
Different lignocellulosic biomasses are found worldwide and each country has its own important industrial crop that can be converted into high-value products, such as ethanol. Therefore, evaluation of new to used in biorefineries is decrease the dependence on non-renewable resources guarantee sustainable development. This work evaluated Brachiaria brizantha, a grass commonly animal forage, standard biomass for 2G-ethanol, sugarcane bagasse. The chemical compositions both were determined...