A5027273975- Optical measurement and interference techniques
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced Measurement and Metrology Techniques
- Analytical Chemistry and Chromatography
- Adaptive optics and wavefront sensing
- Surface Roughness and Optical Measurements
- Machine Learning in Bioinformatics
- Antifungal resistance and susceptibility
- Advanced optical system design
- Botany and Plant Ecology Studies
- Algorithms and Data Compression
- Forest Ecology and Biodiversity Studies
- Optical Polarization and Ellipsometry
- Quantum optics and atomic interactions
- Advanced Clustering Algorithms Research
- Mycotoxins in Agriculture and Food
- Environmental Science and Water Management
- Environmental Chemistry and Analysis
- Peatlands and Wetlands Ecology
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Per- and polyfluoroalkyl substances research
- Microbial Natural Products and Biosynthesis
Czech Academy of Sciences, Institute of Microbiology
2015-2024
Czech Academy of Sciences
2006-2024
Czech Technical University in Prague
2005-2024
Forestry and Game Management Research Institute
2021-2024
Charles University
2010-2022
Agricultural Research Institute Kromeriz
2005-2018
Národný Onkologický Ústav
2013
ADVERTISEMENT RETURN TO ISSUEPREVFeatureNEXTStructural Analysis of Natural ProductsCurrent mass spectrometry, nuclear magnetic resonance spectroscopy, and X-ray diffraction are presented as structure elucidation tools for analytical chemistry natural products. Discovering new molecular entities combined with dereplication known organic compounds represent prerequisites biological assays respective applications pharmaceuticals or markers. Liquid chromatography is briefly addressed respect to...
Nonribosomal peptides have a wide range of biological and medical applications. Their identification by tandem mass spectrometry remains challenging task. A new open-source de novo peptide engine CycloBranch was developed successfully applied in or detailed characterization 11 linear, cyclic, branched, branch-cyclic peptides. is based on annotated building block databases the size which defined user according to ribosomal nonribosomal origin. The current number involved nonisobaric isobaric...
Abstract Invasive pulmonary aspergillosis results in 450,000 deaths per year and complicates cancer chemotherapy, transplantations the treatment of other immunosuppressed patients. Using a rat model experimental aspergillosis, fungal siderophores ferricrocin triacetylfusarinine C were identified as markers quantified urine, serum lung tissues. Biomarkers analyzed by matrix-assisted laser desorption ionization (MALDI) electrospray mass spectrometry using 12T SolariX Fourier transform ion...
Natural product chemistry, microbiology, and food, human, plant metabolomics represent a few sources of complex data generated by mass spectrometry. Among the medley software tools used to handle these sets, no universal tool can qualitatively, quantitatively, or statistically address major biological questions tasks. CycloBranch 2, an open platform-free software, at least now provides de novo generation molecular formulas unknown compounds in both liquid chromatography/mass spectrometry...
In acutely ill patients, particularly in intensive care units or mixed infections, time to a microbe-specific diagnosis is critical successful outcome of therapy. We report the application evolving technologies involving mass spectrometry diagnose and monitor patient’s course. As proof this concept, we studied five patients used two rat models mono-infection coinfection. noninvasive combined monitoring Aspergillus fumigatus Pseudomonas aeruginosa infection. The invasive coinfection was...
Abstract The quadrature of general, highly oscillatory integrals is a relatively complicated computational problem that occurs in wide range practical applications, e.g. physics, chemistry, and image analysis. It often necessary to use high number nodal points with classical formulas order achieve required accuracy numerical integration rapidly oscillating functions. However, an increase leads larger time. Our work describes analyses method for functions, which enables obtain the smaller...
Our work is focused on a problem of numerical evaluation Zernike polynomials and their Cartesian partial derivatives. Since the direct calculation using explicit definition relations relatively slow it numerically instable for higher orders evaluated there need more effective stable method. In recent years several recurrent methods were developed polynomials. These are up to very high they much faster than calculation. our brief review existing given then an analogous recurrence method x y...
A procedure for processing frozen rat lung tissue sections scanning electron microscopy (SEM) from deeply samples initially collected and stored matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed. The employed slow thawing of the while floating on surface melting in a fixative solution. After float-washing step, were dehydrated graded ethanol series dried critical point dryer. SEM generated images with well-preserved structures, allowing...
In biological applications, the tandem mass spectrometry is a widely used method for determining protein and peptide sequences from an "in vitro" sample. The are not determined directly, but they must be interpreted spectra, which output of spectrometer. This work focused on similarity-search approach to spectra interpretation, where parameterized Hausdorff distance (dHP) as similarity. order provide efficient similarity search under dHP, metric access methods TriGen algorithm (controlling...
Abstract Nonribosomal peptides and polyketides are natural products commonly synthesized by microorganisms. They widely used in medicine, agriculture, environmental protection, other fields. The structures of often analyzed high-resolution tandem mass spectrometry, which becomes more popular with its increasing availability. However, the characterization nonribosomal from spectra is a nontrivial task because they composed many uncommon building blocks addition to proteinogenic amino acids....
Abstract Within the growing community of Fourier transform mass spectrometry users, identification fine isotope structure has become an indispensable method for molecular formula determination. In this work, envelopes accessing mutual ratio 2 closely related pyoverdines in a mixture were used. Bacterial siderophores D and E cannot be easily separated via liquid chromatography‐mass because their structures differ (de)amidation at respective chromophore parts only. Their was determined nuclear...
Mass spectrometry is a very popular method for protein and peptide identification nowadays. Abundance of data generated in this way grows exponentially every year. Although there exist algorithms interpreting mass spectra, demand faster more accurate approaches remains. We propose an approach preprocessing the sequence database based on metric access methods. This allows to select only small set suitable candidates, which can be then compared with experimental spectra using sophisticated...
In biological applications, the tandem mass spectrometry is a widely used method for determining protein and peptide sequences from an in vitro sample. The are not determined directly, but they must be interpreted spectra, which output of spectrometer. This work focused on similarity-search approach to spectra interpretation, where parametrized Hausdorff distance (dHP) as similarity. order provide efficient similarity search under dHP, metric access methods TriGen algorithm (controlling...