A5050284391- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Cancer Treatment and Pharmacology
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Bioactive Natural Diterpenoids Research
- Phytochemical compounds biological activities
- Agriculture and Biological Studies
- Natural Compounds in Disease Treatment
- Microtubule and mitosis dynamics
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Enzyme Catalysis and Immobilization
- Natural product bioactivities and synthesis
- Plant and animal studies
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Plant-based Medicinal Research
- 14-3-3 protein interactions
- Banana Cultivation and Research
- Biological Activity of Diterpenoids and Biflavonoids
- Bioeconomy and Sustainability Development
Universitat Pompeu Fabra
2007-2015
VIB-UGent Center for Plant Systems Biology
2014
Ghent University
2014
Summary Methyl jasmonate and cyclodextrins are proven effective inducers of secondary metabolism in plant cell cultures. Cyclodextrins, which cyclic oligosaccharides, can form inclusion complexes with nonhydrophilic products, thus increasing their excretion from the producer cells to culture medium. In present work, using a selected Taxus x media line cultured two‐stage system, relationship between taxane production transcript profiles several genes involved taxol was studied gain more...
Dedifferentiated Taxus media cell cultures presenting the same genetic characteristics as parent culture were established from transformed roots. Two lines studied: Rol C, carrying T-DNA of A. rhizogenes 9,402 and TXS, both txs transgene T. baccata under control 35S CaMV promoter. In second part a previously optimized two-stage system, cultured in production medium supplemented with elicitor methyl jasmonate. Taxane was compared an untransformed line conditions. The highest taxane observed...
Taxol (paclitaxel) and its derivatives are microtubule-stabilizing drugs widely used in the treatment of several types cancer, including mammary, prostate, ovarian non-small-cell lung carcinoma, as well AIDS-associated Kaposi's sarcoma other tumor. Taxanes stabilize microtubules by enhancing their polymerization inhibiting depolymerization. Microtubule dynamics crucial to mitotic spindle formation function; therefore, cells exposed taxanes unable undergo chromosomal separation during...
Summary Plant cell cultures constitute eco‐friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well a suitable system extending our knowledge metabolism. Despite high added value taxol and importance taxanes anticancer compounds, several aspects their biosynthesis remain unknown. In this work, genomewide expression analysis jasmonate‐elicited Taxus baccata by complementary DNA ‐amplified fragment length polymorphism ( cDNA...
Summary Small peptides play important roles in the signalling cascades that steer plant growth, development and defence, often crosstalk with hormonal signalling. Thereby, they also modulate metabolism, including production of bioactive molecules are high interest for human applications. Yew species ( T axus spp.) produce diterpenes such as powerful anticancer agent paclitaxel, biosynthesis which can be stimulated by hormone jasmonate, both whole plants cell suspension cultures. Here, we...
Being native from Morocco and found mostly on the slopes of Atlas, Euphorbia resinifera, a succulent Euphorbiaceae, was used in antiquity as medicinal plant by applying its dried latex (Euphorbium) against snake bites during poisoning. More recently, this shown to contain strongest “spice” nature so far, diterpenoid resiniferatoxin (RTX) [1]. This complex molecule is 1000 more potent Scoville scale than pure capsaicin RTX, which strongly activate TRPV1 receptors neurons, now being tested...
The production and accumulation of secondary metabolites in plants is always regulated by the expression genes involved their biosynthesis. There are very few reports about regulation biosynthesis anticancer agent taxol other related taxanes rate-limiting steps involved, especially during development Taxus plants. Using baccata L. plantlets grown vitro for 1 year, our group has studied relationship between profile codifying enzymes that participate early late taxane (TXS, DBAT, BAPT DBTNBT)....
Preparations of Tripterygium have been used in traditional Chinese medicine for years. Nowadays, close to 400 secondary metabolites reported from species, with 95% being terpenoids. One the most active terpenoids isolated is celastrol which has treatment asthma, chronic inflammation, and autoimmune neuro-degenerative diseases [1]. Celastrol shown very powerful anti-cancer activity many cancer types, including leukemia, glioma, melanoma, prostate cancer, breast various mechanisms. Even such a...
Leontopodium alpinum, better know as Edelweiss, is a protected asteraceae species only found in mountains above 1800 m of altitude. This delicate plant has received some interest from the cosmetic industry due to its metabolites, and particular leontopodic acids, which are capable providing antioxidant anti-inflammatory benefit for human skin. The protection along with specific growing environment bottleneck large-scale production active ingredients based on this species. To circumvent...