A5044017677- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Toxin Mechanisms and Immunotoxins
- Helicobacter pylori-related gastroenterology studies
- Nosocomial Infections in ICU
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Gastrointestinal motility and disorders
- Genomics and Phylogenetic Studies
- Parasite Biology and Host Interactions
- Antibiotic Resistance in Bacteria
- Plant-Microbe Interactions and Immunity
- Celiac Disease Research and Management
- Nematode management and characterization studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Community Ecology and Physiology
- Infant Health and Development
- Legume Nitrogen Fixing Symbiosis
- Orthopedic Infections and Treatments
- Enterobacteriaceae and Cronobacter Research
University of Maribor
2016-2025
National Laboratory of Health, Environment and Food
2016-2025
European Society of Clinical Microbiology and Infectious Diseases
2017-2024
Faculty (United Kingdom)
2024
University of Leeds
2017-2023
Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins
2011-2018
Institute for Environmental Protection and Sensors
2009-2018
Leeds Teaching Hospitals NHS Trust
2017
Institute of Public Health Zadar
2012
Istituto Superiore di Sanità
2011
In addition to the two large clostridial cytotoxins (TcdA and TcdB) certain strains of Clostridium difficile produce an actin-specific ADP-ribosyltransferase, or binary toxin. PCR reactions were developed detect genes encoding enzymatic (cdtA) binding (cdtB) components toxin 170 representative tested assess prevalence Positive results (n=59) confirmed by immunoblotting ADP-ribosyltransferase assay. ribotype toxinotype (restriction fragment length polymorphism analysis for TcdA correlated...
ABSTRACT Two hundred nineteen Clostridium difficile isolates from 22 serogroups were screened for changes in the genes coding toxin B ( tcdB ) and A tcdA ). Parts of amplified, PCR fragments checked length polymorphisms cut with several restriction enzymes to monitor fragment (RFLPs). For 47 strains (21%), differences found compared reference strain VPI 10463. Polymorphisms usually observed both genes. RFLPs more commonly gene, which a single enzyme could give up five different patterns....
Binary toxin CDT or its genes have been identified in some strains of Clostridium difficile that also produce the large clostridial toxins, toxins A and B (A+B+CDT+), including a newly recognized epidemic strain United States Canada. To study effects binary alone, we characterized 4 CDT-positive only (A-B-CDT+) C. strains. Unlike other required exogenous trypsin for activation. Supernatants from all A-B-CDT+ caused marked fluid accumulation rabbit ileal loop assay after concentration...
Multidrug resistance and antibiotic mechanisms were investigated in 316 Clostridium difficile clinical isolates collected during the first European surveillance on C. 2005. MICs of eight different antibiotics determined using Etest. Reserpine- carbonyl cyanide m-chlorophenylhydrazone-sensitive efflux was tested agar dilution method. Molecular analysis performed PCR assays, mapping sequencing. One hundred forty-eight strains resistant to at least one 82 (55%) multidrug resistant. In...
Endophytic bacteria from wooden plants and especially seed-associated endophytes are not well studied. Fresh seeds collected four Norway spruce trees (Picea abies) different locations in the Slovene subalpine region were surface-sterilised dissected into a seed coat, embryo endosperm. The presence of was detected by culturing methods direct amplification eubacterial 16S rDNA gene. Both approaches identified genera Pseudomonas Rahnella seeds. known plant-associated with growth-promoting...
ABSTRACT The emergence of Clostridium difficile as a significant human diarrheal pathogen is associated with the production highly transmissible spores and acquisition antimicrobial resistance genes (ARGs) virulence factors. Unlike hospital-associated C. RT027 lineage, community-associated RT078 lineage isolated from both humans farm animals; however, geographical population structure transmission networks remain unknown. Here, we applied whole-genome phylogenetic analysis 248 strains 22...
Clostridium difficile is an important cause of intestinal infections in some animal species and animals might be a reservoir for community associated human infections. Here we describe collection C. strains from 12 countries based on inclusion criteria one strain (PCR ribotype) per laboratory. Altogether 112 isolates were collected distributed into 38 PCR ribotypes with agarose approach 50 sequencer approach. Four most prevalent terms number as well different host species: 078 (14.3%...
Abstract Background Characterising the overlap of C. difficile genotypes in different reservoirs can improve our understanding possible transmission routes this pathogen. Most studies have focused on a comparison PCR ribotype 078 isolated from humans and animals. Here we describe for first time during longer intervals sources including humans, animals non-hospital environment. Results Altogether 786 isolates interval 2008-2010 were grouped into 90 ribotypes eleven them shared among all host...
Clostridium difficile is one of the most important human and animal pathogens. However, bacterium ubiquitous can be isolated from various sources. Here we report prevalence characterization C. in less studied environmental samples, puddle water (n = 104) soil 79). was detected 14.4% 36.7% samples. Environmental strains displayed antimicrobial resistance patterns comparable to already published data isolates. A total 480 isolates were grouped into 34 different PCR ribotypes. More than half...
Genetic variants of Clostridium difficile have been reported with increasing frequency, but their true incidence is unknown. C. strains classified into variant toxinotypes according to variations in the pathogenicity locus encoding major virulence factors, toxins A and B. Some produce an additional toxin, binary toxin CDT. This survey clinical isolates (153) from patients a single hospital set out ascertain distribution possessing genes. PCR-RFLP-based method toxinotyping identified 123...
ABSTRACT A total of 56 C. difficile strains were selected from 310 isolates obtained different hospitals in Japan and Korea healthy infants Indonesia. Strains that had been previously typed by pulsed-field gel electrophoresis PCR ribotyping, characterized toxinotyping binary toxin gene detection. When toxinotyped, 35 determined to be toxinotype 0, whereas 21 showed variations genes could grouped into 11 variant toxinotypes. Six the toxinotypes described before (I, III, IV, VIII, IX, XII). In...
Clostridium difficile is a major nosocomial pathogen that produces two large protein toxins [toxin A (TcdA) and toxin B (TcdB)] capable of disrupting intestinal epithelial cells. Both belong to the family clostridial cytotoxins, which are characterized by presence repetitive C-terminal domain (CRD). In TcdA, CRD composed 39 repeats responsible for binding cell surface carbohydrates. To understand molecular structural basis from C. , we have determined 1.85-Å resolution crystal structure...
The germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation kernel-associated bacterial endophytes. Bacterial strains identified by partial 16S rRNA gene sequencing as Pantoea sp., Microbacterium Frigoribacterium Bacillus Paenibacillus and Sphingomonas sp. were isolated from 4 different cultivars. Genus was associated with specific cultivar. this cultivar often overgrown the fungus Lecanicillium aphanocladii ; however, those...