Abstract Background Pseudofactin II is a recently identified biosurfactant secreted by Pseudomonas fluorescens BD5, the strain obtained from freshwater Arctic Archipelago of Svalbard. novel compound as cyclic lipopeptide with palmitic acid connected to terminal amino group eighth in peptide moiety. The C-terminal carboxylic last forms lactone hydroxyl Thr3. Adhesion first stage biofilm formation and best moment for action antiadhesive anti-biofilm compounds. Adsorption biosurfactants surface...

Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of production processes can be achieved, in part, by cheap substrates found among agricultural food wastes or byproducts. In the present study, crude glycerol, a raw material from several was evaluated as potential low-cost carbon source to reduce costs surfactin Bacillus subtilis #309. The medium containing soap-derived waste glycerol led best...

Food waste (FW) to value-added application represents a demanding opportunity in the circular economy. As one of approaches, FW hydrothermal carbonization (HTC) delivers an aqueous phase (HTC-AP), which would be suitable when applied microbial growth and lipid production. In this context, current work explored HTC-AP from obtained through (temperatures 200 260˚C) for 14 different Yarrowia species. Among these strains, Y. lipolytica, keelungensis, porcina, galii showed ability utilize HTC-AP....

Erythritol is a natural sweetener that used in the food industry. It produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change insulin level blood, therefore can be safely diabetics. Previously, has been shown erythrose reductase (ER), which catalyzes final step, plays crucial role erythritol synthesis. ER reduces with NAD(P)H cofactor. Despite many studies on synthesis Yarrowia lipolytica, enzymes involved this metabolic pathway have ever...

Rhodococcus fascians BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product identified trehalose lipid with a molecular mass of 848 g mol-1. purified reduced surface tension water 72 34 mN m-1. critical micelle concentration 0.140 mg mL-1. To examine its potential for biomedical applications, antimicrobial and antiadhesive activity evaluated against several pathogenic microorganisms. Trehalose showed...

The microbial conversion of agro-industrial oil wastes into biosurfactants shows promise as a biomass refinery approach. In this study, Bacillus subtilis #309 was applied to produce surfactin using rapeseed and sunflower cakes, the most common processing side products in Europe. Studies chemical composition substrates were performed, determine feasibility cakes for production. Initially, screening proteolytic lipolytic activity performed establish capability B. substrate utilization hence...

In the case of melanoma, advances in therapies are slow, which raises need to evaluate new therapeutic strategies and natural products with potential cancer cell inhibiting effect. Pseudofactin II (PFII), a novel cyclic lipopeptide biosurfactant has been isolated from Arctic strain Pseudomonas fluorescens BD5. The aim this study was investigate effect PFII on A375 melanoma cells compared Normal Human Dermis Fibroblast (NHDF) elucidate underlying mechanism cytotoxic activity. Melanoma NHDF...

The agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) sole substrate, B. E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount in standard medium, contains glycerol, peptone, meat extract. When was supplemented with OMW (10% (v/v)), further increased up to 253 mg/L flasks 269 bioreactor....

Surfactin is a type of cyclic lipopeptide biosurfactant implicated in wide range applications. Although its antimicrobial activity has been characterized, effect on Candida albicans physiology remains to be elucidated. The present study evaluated the influence surfactin-C15 (SF) and complexes with divalent counterions C. biofilm formation preformed biofilms. SF metal(II)-SF inhibited reduced metabolic mature biofilms concentration-dependent manner. same concentrations compounds studied...

Abstract Background Contemporary biotechnology focuses on many problems related to the functioning of developed societies. Many these are health, especially with rapidly rising numbers people suffering from civilization diseases, such as obesity or diabetes. One factor contributing development diseases is high consumption sucrose. A very promising substitute for this sugar has emerged: polyhydroxy alcohols, characterized by low caloric value and sufficient sweetness replace table in food...

The aim of this study was to obtain new, safe, and effective compounds with anticancer activity since cancer is still the leading cause mortality worldwide. rational design new based on introduction differentially substituted phenylpiperazines into 1,2-benzothiazine scaffold as a reference for structures recent topoisomerase II (Topo II) inhibitors such dexrazoxane XK-469. newly designed group derivatives synthesized tested healthy (MCF10A) (MCF7) cell lines, alone in combination doxorubicin...

Fragments of MUC7, a salivary protein involved in nonimmune defense, arise from proteolytic cleavage saliva and exhibit antimicrobial properties. However, their therapeutic use is limited by low stability due to further degradation. To address this, native MUC7 fragment was modified using d-amino acids the retro-inverso strategy. Given role metal ions enhancing peptides, we analyzed bioinorganic chemistry these systems with Cu(II) assessed activity against fungal bacterial strains. This...

This work introduces a novel class of hybrid antimicrobial agents by integrating sulfone and sulfonamide functionalities with polyhedral oligomeric silsesquioxanes (POSSs). By employing efficient synthetic protocols, we have successfully prepared both (ethylvinylsulfone-POSS phenylethylsulfone-POSS) (benzenesulfonamide-POSS, p-toluenesulfonamide-POSS, 3-fluorobenzenesulfonamide-POSS, 2-naphthalenesulfonamide-POSS) derivatives high yields (73–90%). All were examined using Fourier transform...

Biofilms formed by Candida albicans pose therapeutic challenges due to their resistance conventional antimicrobials, highlighting the need for more effective treatments. Rhamnolipids (RLs) are biosurfactants with diverse antimicrobial properties. Bacteriophages viruses that target specific bacterial strains. Recent studies have shown they may affect biofilm formation fungi and yeasts. This study investigated combined effects of RLs bacteriophages against C. biofilms, focusing on...

Proteolytic enzymes are commercially valuable and have multiple applications in various industrial sectors. The most studied proteolytic produced by Yarrowia lipolytica, extracellular alkaline protease (Aep) acid (Axp), were shown to be good candidates for different biotechnological applications. In this study, we performed a comprehensive analysis of the clade species, including phylogenetic studies, synteny analysis, production application. Using combination comparative genomics approaches...

Stearin-derived waste glycerol as an economic substrate for biosurfactant production using Pseudomonas antarctica isolated from the Arctic Archipelago of Svalbard.

Bacillus velezensis P#02 simultaneously produced surfactin and poly–γ–glutamic acid (γ–PGA). Among the different culture media studied, one containing corn steep liquor (100 mL/L), glucose (10 g/L), glutamic g/L) as sole ingredients (CSL–G–Glut(10)) offered best results regarding biosurfactant biopolymer production. Although production occurred both under shaking static conditions, significant only in cultures. Using medium CSL–G–Glut(10), 910 ± 20 mg surfactin/L 9.8 0.2 g γ–PGA/L were...