A5090884144- Nitric Oxide and Endothelin Effects
- Organ Transplantation Techniques and Outcomes
- Pediatric Hepatobiliary Diseases and Treatments
- Metastasis and carcinoma case studies
- Transplantation: Methods and Outcomes
- Gastric Cancer Management and Outcomes
- Liver Disease and Transplantation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Helicobacter pylori-related gastroenterology studies
- Protease and Inhibitor Mechanisms
- Tracheal and airway disorders
- Gallbladder and Bile Duct Disorders
- Respiratory Support and Mechanisms
- Congenital Anomalies and Fetal Surgery
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gastrointestinal Tumor Research and Treatment
- Peptidase Inhibition and Analysis
- Pulmonary Hypertension Research and Treatments
- Medical Imaging and Pathology Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Polyomavirus and related diseases
- Mechanical Circulatory Support Devices
- Diabetes and associated disorders
- Renal Transplantation Outcomes and Treatments
- Neuroendocrine Tumor Research Advances
National Center for Global Health and Medicine
2014-2025
Kyoto University Hospital
2011-2025
Kyoto University
2011-2024
Center for Global Health
2024
University of Hyogo
2022
DIC (Japan)
2022
Japan Pediatric Society
2020-2021
Nagoya University
2018-2021
Fukuoka Tokushukai Hospital
2014-2020
The Japanese Society of Gastroenterological Surgery
2020
Oxidative stress may cause tissue injury through activation of the precursors matrix metalloproteinase (proMMPs). In this study, we observed glutathione (GSH)-dependent proMMP induced by peroxynitrite, a potent oxidizing agent formed during inflammatory processes. Peroxynitrite strongly activated all three types purified human proMMPs (proMMP-1, -8, and -9) in presence similar concentrations GSH. Of potential reaction products between peroxynitrite GSH, only S-nitroglutathione (GSNO(2))...
Rationale: More than 25 million individuals have heart failure worldwide, with ≈4000 patients currently awaiting transplantation in the United States. Donor organ shortage and allograft rejection remain major limitations only ≈2500 hearts transplanted each year. As a theoretical alternative to allotransplantation, patient-derived bioartificial myocardium could provide functional support ultimately impact treatment of failure. Objective: The objective this study is translate previous work...
Destruction of subepithelial basement membrane is a key event in the pathogenesis idiopathic pulmonary fibrosis (IPF). To evaluate role matrix metalloproteinases (MMPs) parenchymal remodeling interstitial pneumonia (IIP), we studied MMP-2 and -9 activity, bronchoalveolar lavage fluid (BALF) by zymography expression TIMP-2 lung tissue immunohistochemistry. BALF tissues were collected from 26 patients with usual (IPF-UIP), 11 nonspecific (NSIP), 6 bronchiolitis obliterans organizing (BOOP)....
Ceruloplasmin (CP) is a major multicopper-containing plasma protein that not only involved in iron metabolism through its ferroxidase activity but also functions as an antioxidant. However, physiological substrates for CP have been fully identified nor has the role of understood. The reaction nitric oxide (NO) with was investigated view nitrosothiol (RS-NO) formation. First, formation heavy metal- or CP-catalyzed RS-NO examined physiologically relevant concentrations NO and various thiol...
A nitrated guanine nucleotide, 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP), is formed via nitric oxide (NO) and causes protein <i>S</i>-guanylation. However, intracellular 8-nitro-cGMP levels mechanisms of formation <i>S</i>-guanylation are yet to be identified. In this study, we precisely quantified NO-dependent in C6 glioma cells liquid chromatography-tandem mass spectrometry. Treatment with <i>S</i>-nitroso-<i>N</i>-acetylpenicillamine led a rapid, transient increase cGMP,...
Peroxynitrite (ONOO ‐), which is generated from nitric oxide (NO) and superoxide anion (O 2 ‐) under pathological conditions, plays an important role in pathophysiological processes. Activation of matrix metalloproteinases (MMPs) contributes to tumor angiogenesis metastasis. NO mediates the enhanced vascular permeability retention (EPR) effect solid tumors, ONOO‐ activates proMMP MMP vitro. In this study, we examined EPR tumors normal tissues as related activation. Authentic ONOO‐, at 50...
A highly stereoselective catalytic alkylation sequence for the synthesis of functionalized and versatile five-membered-ring compounds bearing all-carbon quaternary stereocenters was developed. Enantioselective desymmetrization achiral cyclopentene-1,3-diones thus executed by chiral Cu–phosphoramidite catalysts. variety complicated cyclopentane derivatives can be synthesized with excellent stereoselectivities using a low catalyst loading in one-pot operation.
Acute lung inflammation and injury were induced by intranasal instillation of lipopolysaccharide (LPS) in normal type 2 nitric oxide synthase (NOS2)-deficient (NOS2 -/- ) C57BL/6 mice. LPS-induced increases extravasated airway neutrophils lavage fluid TNF-α macrophage inflammatory protein-2 markedly lower NOS2 than wild-type mice, indicating that NOS2-derived (NO·) participates cytokine production neutrophil recruitment. Instillation LPS also increased total protein matrix...
At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had sudden onset high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds completely healed, without complicated infections, when occurred. clinical picture in all was atypical: diffuse salmon-pink skin color, heat, swelling, spontaneous pain, tenderness but no eruptions. No patient any underlying immunocompromising...
Laparoscopic surgery for periampullary disease is still a challenging operation. The aim of this study was to compare the perioperative outcomes patients undergoing conventional pancreaticoduodenectomy (PD) with those laparoscopy-assisted PD.A retrospective analysis conducted on 51 consecutive who underwent or open PD disease.There were no significant differences in preoperative demographic clinical data two groups. Although there operative time between groups, blood loss group significantly...
8-Nitro-cGMP (8-nitroguanosine 3′,5′-cyclic monophosphate) is a nitrated derivative of cGMP, which can function as unique electrophilic second messenger involved in regulation an antioxidant adaptive response cells. In the present study, we investigated chemical and biochemical regulatory mechanisms 8-nitro-cGMP formation, with particular focus on roles ROS (reactive oxygen species). Chemical analyses demonstrated that peroxynitrite-dependent oxidation myeloperoxidase-dependent nitrite...