A5046557008- Platelet Disorders and Treatments
- Advanced Control Systems Design
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Bioactive Natural Diterpenoids Research
- Phytochemistry and Biological Activities
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Fuzzy Logic and Control Systems
- Blood properties and coagulation
- Mitochondrial Function and Pathology
- T-cell and B-cell Immunology
- Fractional Differential Equations Solutions
- Antiplatelet Therapy and Cardiovascular Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Gut microbiota and health
- Biological Activity of Diterpenoids and Biflavonoids
- RNA Interference and Gene Delivery
- Lymphatic System and Diseases
- Animal Virus Infections Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Extremum Seeking Control Systems
- Adipokines, Inflammation, and Metabolic Diseases
University of Cambridge
2022-2025
University of Birmingham
2020-2025
Northeastern University
2023-2024
Shaanxi Provincial People's Hospital
2024
The Gurdon Institute
2022-2024
Capital Normal University
2023
University of Oxford
2009-2011
Kunming Institute of Botany
2010
Chinese Academy of Sciences
2010
MRC Human Immunology Unit
2009
Type 5 adenovirus (Ad5) is a human pathogen that has been widely developed for therapeutic uses, with only limited success to date. We report here the novel finding erythrocytes present Coxsackie virus-adenovirus receptor (CAR) providing an Ad5 sequestration mechanism protects against systemic infection. Interestingly, from neither mice nor rhesus macaques CAR. Excess fiber protein or anti-CAR antibody inhibits binding of and cryo-electron microscopy shows attachment via Ad5, leading close...
To better understand the mechanisms of intracellular trafficking and presentation exogenous peptides by antigen-presenting cells (APC), we compared handling overlapping 24-mer from HIV Nef either mixed or covalently linked in tandem one protein. Once internalized, trafficked not only to endosomes but also cytosol, activated CD8<sup>+</sup> CD4<sup>+</sup> T cells. In contrast, whole protein was found traffic endosomal compartments, primarily Finally, with adjuvant, were capable protecting...
The collagen receptor glycoprotein VI (GPVI) is an attractive antiplatelet target due to its critical role in thrombosis but minor involvement hemostasis.To investigate GPVI platelet activation by collagen-I and atherosclerotic plaque using novel blocking non-blocking anti-GPVI nanobodies (Nbs).Nb effects on GPVI-mediated signaling function were assessed western blot whole blood thrombus formation under flow. clustering was visualized thrombi fluorescently labeled Nb28.Under arterial shear,...
Appropriate cellular recognition of viruses is essential for the generation an effective innate and adaptive immune response. Viral sensors their downstream signaling components thus provide a crucial first line host defense. Many them exhibit subcellular relocalization upon activation, resulting in expression interferon antiviral genes. To comprehensively identify factors, we analyzed protein on global scale during viral infection. cAMP-responsive element-binding (CREB)-regulated...
Abstract Background and Purpose The C‐type lectin‐like receptor‐2 (CLEC‐2) is a platelet receptor for the endogenous ligand podoplanin. This interaction contributes to several (patho)physiological processes, such as lymphangiogenesis, preservation of blood lymphatic vessel integrity organ development, tumour metastasis. Activation CLEC‐2 leads phosphorylation its cytoplasmic hemITAM domain initiates signalling cascade involving kinase Syk. aim this study was identify characterise novel small...
Obesity is associated with a proinflammatory and prothrombotic state that supports atherosclerosis progression. The goal of this study was to gain insights into the phosphorylation events related platelet reactivity in obesity identify biomarkers altered activation pathways clinical condition. Approach Results: We performed comparative phosphoproteomic analysis resting platelets from obese patients their age- gender-matched lean controls. data were validated by mechanistic, functional,...
Abstract CLEC-2 is a target for new class of antiplatelet agent. Clustering leads to phosphorylation cytosolic YxxL and binding the tandem SH2 domains in Syk, crosslinking two receptors. We have raised 48 nanobodies crosslinked most potent these generate divalent tetravalent nanobody ligands. Fluorescence correlation spectroscopy (FCS) was used show that multivalent cluster membrane clustering reduced by inhibition Syk. Strikingly, stimulated aggregation human platelets, whereas an...
Abstract Modified vaccinia Ankara (MVA) virus does not replicate in human cells and is the vaccine deployed to curb current outbreak of mpox. Here, we conduct a multiplexed proteomic analysis quantify >9000 cellular ~80% viral proteins throughout MVA infection fibroblasts macrophages. >690 are down-regulated >2-fold by MVA, revealing substantial remodelling host proteome. >25% these targets shared with replication-competent vaccinia. Viral intermediate/late gene expression...
Mechanisms that safeguard mitochondrial DNA (mtDNA) limit the accumulation of mutations linked to and age-related diseases. Yet, pathways repair double-strand breaks (DSBs) in animal mitochondria are poorly understood. By performing a candidate screen for mtDNA proteins, we identify REC-an MCM helicase drives meiotic recombination nucleus-also localizes Drosophila. We show REC repairs DSBs by homologous somatic germline tissues. Moreover, prevents age-associated mutations. further MCM8,...
Abstract Genetic screens have been used extensively to probe interactions between nuclear genes and their impact on phenotypes. Probing mitochondrial phenotypic outcome, however, has not possible due a lack of tools map the responsible polymorphisms. Here, using toolkit we previously established in Drosophila , isolate over 300 recombinant genomes naturally occurring polymorphism at cytochrome c oxidase III residue 109 (CoIII ) that fully rescues lethality other defects associated with point...
The paper focuses on the admissibility problem of descriptor fractional-order systems (DFOSs). alternate criteria are addressed for DFOSs with order in (0,2) which involve a non-strict linear matrix inequality (LMI) method and strict LMI method, respectively. forms LMIs brand new distinguished existing literature, fills gaps studies admissibility. These necessary sufficient conditions available to without separating ranges into (0,1) [1,2). Based special position singular matrix, criterion...
Human rickettsioses are worldwide zoonoses and it is not easy to differentiate them from other infectious diseases because of their atypical manifestation. In recent years the number patients with fever unknown causes Hongta District CDC, Yuxi city Yunnan Province has been increasing significantly in summer. Diagnosis scrub typhus was made by local clinicians. order ascertain disease, we undertook a laboratory investigation for such August 18 26, 2005.Active surveillance conducted CDC 2002...
The paper focuses on the admissibility problem of descriptor fractional-order systems (DFOSs). alternate criteria are addressed for DFOSs with order in (0,2) which involve a non-strict linear matrix inequality (LMI) method and strict LMI method, respectively. forms LMIs brand-new distinguished existing literature, fill gaps studies admissibility. These approaches available to without separating ranges into (0,1) [1,2). In addition, involved least real decision variables terms is derived more...
Abstract Background The novel coronavirus SARS-CoV-2 has caused a global pandemic. Vaccines are an important part of the response. Although rare, unusual thrombotic events and thrombocytopenia in recipients 4–16 days after vaccination with AstraZeneca AZD1222 have been reported. This syndrome vaccine-induced immune (VITT) clinically resembles heparin induced (HIT), which is by platelet activating antibodies against factor 4 (PF4). Here, we investigate effect serum from patients VITT on...
ABSTRACT Appropriate cellular recognition of viruses is essential for the generation effective innate and adaptive antiviral immunity. Viral sensors their signalling components thus provide a crucial first line host defence. Many exhibit subcellular relocalisation upon activation, triggering expression interferon genes. To identify novel factors we analysed protein on global scale during viral infection. CREB Regulated Transcription Coactivators-2 3 (CRTC2/3) exhibited early...
Appropriate cellular recognition of viruses is essential for the generation effective innate and adaptive antiviral immunity. Viral sensors their signalling components thus provide a crucial first line host defence. Many exhibit subcellular relocalisation upon activation, triggering expression interferon genes. To identify novel factors we analysed protein on global scale during viral infection. CREB Regulated Transcription Coactivators-2 3 (CRTC2/3) exhibited early cytoplasmic-to-nuclear...