Marcell Zimanyi
A5079720432- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Molecular Communication and Nanonetworks
- Blood Coagulation and Thrombosis Mechanisms
- Advanced Biosensing Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Advancements in Semiconductor Devices and Circuit Design
- Advanced biosensing and bioanalysis techniques
- Protease and Inhibitor Mechanisms
- Nanowire Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Viral gastroenteritis research and epidemiology
University of California, San Francisco
2020-2025
Howard Hughes Medical Institute
2020
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus enters host cells via an interaction between its Spike protein and the cell receptor angiotensin-converting enzyme (ACE2). By screening a yeast surface-displayed library of synthetic nanobody sequences, we developed nanobodies that disrupt ACE2. Cryo-electron microscopy (cryo-EM) revealed one nanobody, Nb6, binds in fully inactive conformation with binding domains locked into their inaccessible down state, incapable...
ABSTRACT Without an effective prophylactic solution, infections from SARS-CoV-2 continue to rise worldwide with devastating health and economic costs. gains entry into host cells via interaction between its Spike protein the cell receptor angiotensin converting enzyme 2 (ACE2). Disruption of this confers potent neutralization viral entry, providing avenue for vaccine design therapeutic antibodies. Here, we develop single-domain antibodies (nanobodies) that potently disrupt ACE2. By screening...
Cryo-Electron Microscopy (cryo-EM) is an enabling technology for structure-guided antibody drug design. The 50 kD fragment antigen-binding (Fab) particularly amenable to cryo-EM due its clear features and ability make rigid complexes with antigens. However, the technical challenges of analyzing macromolecular under 100 hinders analysis Fabs bound a large portion druggable genome. We were able determine structure Fab in complex 28 viral protease where entire binding interface (epitope) are...