A5052398473- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- biodegradable polymer synthesis and properties
- Microbial Fuel Cells and Bioremediation
- Microplastics and Plastic Pollution
- Antimicrobial Peptides and Activities
- Microbial metabolism and enzyme function
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Antimicrobial agents and applications
- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- Microbial bioremediation and biosurfactants
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Antibiotic Resistance in Bacteria
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Minerals Flotation and Separation Techniques
- Pickering emulsions and particle stabilization
- Carbon dioxide utilization in catalysis
- Steroid Chemistry and Biochemistry
- Algal biology and biofuel production
Nagoya University
2015-2024
Graduate School USA
2020
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Hashimoto Municipal Hospital
2013
Nakanishi (Japan)
2013
Nagoya Institute of Technology
2004-2011
Japan Science and Technology Agency
2008-2010
Asahi Kasei (Japan)
2010
Tokyo Institute of Technology
1994-2008
ABSTRACT A thermophilic syntrophic bacterium, Pelotomaculum thermopropionicum strain SI, was grown in a monoculture or coculture with hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus ΔH. Microscopic observation revealed that cells of each organism were dispersed independent the growth substrate. In coculture, however, these organisms coaggregated to different degrees depending on substrate; namely, large fraction when they propionate, but relatively few ethanol 1-propanol....
Fibrous materials made of carbon fiber (CF), aromatic polyamide (AP), preoxidized polyacrylonitrile (PAN), and polyethylene (PE), which are widely used in the textile industry, were evaluated as biofilm supports for wastewater treatment. We found that CF has a high capacity adsorbing nitrifying bacterial sludge. The adhesion rate four pure strains-Cytophaga hutchinsonii, Alcaligenes faecalis, Bacillus subtilis, Escherichia coli-was highest to CF. ζ-potentials fibrous supports, cell surface...
Acinetobacter baumannii is a Gram-negative pathogen that causes multitude of nosocomial infections. The trimeric autotransporter adhesin (Ata) belongs to the superfamily adhesins which are important virulence factors in many species. Phylogenetic profiling revealed ata present 78% all sequenced A. isolates but only 2% closely related species calcoaceticus and pittii. Employing markerless deletion mutant ATCC 19606 we show adhesion invasion into human endothelial epithelial cells depend on...
Acinetobacter sp. Tol 5 exhibits an autoagglutinating nature and noteworthy adhesiveness to various abiotic surfaces from hydrophobic plastics hydrophilic glass stainless steel. Although previous studies have suggested that bacterionanofibers on cells are involved in the adhesive phenotype of 5, fiber directly mediates adhesion has remained unknown. Here, we present a new member trimeric autotransporter adhesins designated AtaA, which discovered by analyzing less mutant T1, obtained...
Electron exchange reactions between microbial cells and solid materials, referred to as extracellular electron transfer (EET), have attracted attention in the fields of physiology, ecology, biotechnology. Studies model species iron-reducing, or equivalently, current-generating bacteria such Geobacter spp. Shewanella revealed that redox-active proteins, especially outer membrane c-type cytochromes (OMCs), play a pivotal role EET process. Recent (meta)genomic analyses diverse microorganisms...
ABSTRACT Two morphological types of appendages, an anchor-like appendage and a peritrichate fibril-type appendage, have been observed on cells adhesive bacterium, Acinetobacter sp. strain Tol 5, by use recently developed electron microscopic techniques. The anchor extends straight to the substratum without branching tethers cell body at its end distances several hundred nanometers, whereas fibril attaches in multiple places, fixing much shorter distances.
The toluene-degrading bacterium Acinetobacter sp. Tol 5 shows high adhesiveness mediated by the bacterionanofiber protein AtaA, which is a new member of trimeric autotransporter adhesin (TAA) family. In contrast to other reported TAAs, AtaA mediates adhesion various abiotic surfaces ranging from hydrophobic plastics hydrophilic glass and stainless steel. expression ataA in industrially relevant bacteria improves their enables immobilization directly onto support materials. This represents...
ABSTRACT Environmental isolates are promising candidates for new chassis of synthetic biology because their inherent capabilities, which include efficiently converting a wide range substrates into valuable products and resilience to environmental stresses; however, many remain genetically intractable unamenable established genetic tools tailored model bacteria. Acinetobacter sp. Tol 5, an environmentally isolated Gram-negative bacterium, possesses intriguing properties use in applications....
Toluene-degrading bacteria were isolated from the trickle bed air biofilter (TBAB), which had eliminated toluene efficiently for three months. Among isolates, Acinetobacter genospecies Tol 5 demonstrated highest efficiency in removal. A. tended to adhere hydrophobic materials showing water-repellent. When bacterium was grown on presence of polyurethane foam, almost all cells adhered polymer support until amount 0.12 kg-cell/kg-polyurethane. Analyses cell surface hydrophobicity by...
The feasibility of the simultaneous production polyhydroxyalkanoates (PHAs) and rhamnolipids, as a novel approach to reduce their costs, was demonstrated by cultivation Pseudomonas aeruginosa IFO3924. Fairly large amounts PHAs rhamnolipids were obtained from bacterial cells culture supernatant, respectively. Decanoate more suitable carbon source than ethanol glucose for production, although cell growth without an induction period under pH control. kind affected PHA monomer composition...
ABSTRACT The affinity of microbial cells for hydrophobic interfaces is important because it directly affects the efficiency various bioprocesses, including green biotechnologies. toluene-degrading bacterium Acinetobacter sp. strain Tol 5 has filamentous appendages and a cell surface, shows high adhesiveness to solid surfaces, self-agglutinates. A “bald” mutant this bacterium, T1, lacks decreased but retains surface. We investigated interaction between T1 an organic solvent dispersed in...
For the disruption of a target gene in molecular microbiology, unmarked mutagenesis is preferable to marked because former method raises no concern about polar effect and leaves selection marker. In contrast naturally competent bacteria, there useful for introducing an mutation into large non-competent bacteria. Nevertheless, genes encoding huge proteins exist diverse bacteria are interesting important physiology potential applications. Here we present new such Gram-negative Two replacement...
Methylococcus capsulatus (Bath) is a representative gammaproteobacterial methanotroph that has been studied extensively in diverse research fields. The sacB gene, which encodes levansucrase, causing cell death the presence of sucrose, widely used as counterselectable marker for disruption target gene Gram-negative bacteria. However, not applicable to all bacteria, and its efficiency counterselection M. low. Here, we report construction an alternative marker, pheS*, by introduction two point...