A5006826719- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Circadian rhythm and melatonin
- Ovarian function and disorders
- Birth, Development, and Health
- Genetic and phenotypic traits in livestock
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive biology and impacts on aquatic species
- Hormonal and reproductive studies
- Animal Genetics and Reproduction
- Selenium in Biological Systems
- Reproductive System and Pregnancy
- Gas Sensing Nanomaterials and Sensors
- Dietary Effects on Health
- Neuroendocrine regulation and behavior
- Sunflower and Safflower Cultivation
- Animal Behavior and Welfare Studies
- Infant Nutrition and Health
- Effects of Environmental Stressors on Livestock
- Cell Image Analysis Techniques
- Toxoplasma gondii Research Studies
- Animal Diversity and Health Studies
- Plant Water Relations and Carbon Dynamics
- Polyamine Metabolism and Applications
Universidad de Zaragoza
2016-2025
Instituto Aragonés de Ciencias de la Salud
2010
Center of Molecular Immunology (Cuba)
2004
Abstract: We recently demonstrated the presence of melatonin in ram seminal plasma and differences its concentration this fluid between breeding nonbreeding season. In study, we investigate hypothesis that vitro treatment with affects sperm quality, is reflected fertilization (IVF) results. Semen from nine rams was collected during nonreproductive season treated 1 μ m , 10 n 100 p melatonin. Samples were incubated at 39°C 5% CO 2 motility, viability, capacitation status phosphatidylserine...
Summary Melatonin is a ubiquitous molecule found in wide range of fluids, one them being ram seminal plasma, which it can reach higher concentrations than those blood, suggesting an extrapineal secretion by the reproductive tract. In order to identify source melatonin we first tried determine whether levels were maintained during day. For this purpose, measured plasma obtained from ejaculates six rams at 6:00 a.m. total darkness, 10:00 and 14:00 p.m. The concentration was ( p < 0.05)...
Some breeds of sheep are highly seasonal in terms reproductive capability, and these changes regulated by photoperiod melatonin secretion. These affect the performance rams, impairing semen quality modifying hormonal profiles. Also, antioxidant defence systems seem to be modulated secretion, shows variations. The aim this study was investigate presence testosterone ram seminal plasma their variations between breeding non-breeding seasons. In addition, we analyzed possible correlations...
Contents The effect of melatonin implants administered during non‐breeding season in Rasa Aragonesa rams on sperm motility parameters and other reproductive traits was assessed. In a first experiment, two were implanted (with group M), remaining males as control (C). Semen each collected from 1 May to 23 June, twice or three times week, assessed using computer‐assisted analysis system. Melatonin increased the percentage progressive motile spermatozoa, particularly 46–75 days after...
In the field of assisted reproductive techniques (ART), computer-assisted sperm analysis (CASA) systems have proved their utility and potential for assessing quality, improving prediction fertility a seminal dose. Although most laboratories scientific centers use commercial systems, in recent years certain free open-source alternatives emerged that can reduce costs research groups to face. However, these cannot analyze kinetic responses different stimuli, such as chemotaxis, thermotaxis or...
The reproductive seasonality of sheep suggests that melatonin receptors may be present in ram spermatozoa. study confirms the presence MT1 and MT2 receptors. receptor was detected using immunocytochemistry, with four sperm subpopulations identified based on following labelling patterns: (1) one small subpopulation over entire head tail; (2) two main exhibited reactivity at equatorial, post-acrosomal, neck tail regions; (3) another equatorial only; (4) a which staining only tail....
Some melatonin functions in mammals are exerted through MT1 and MT2 receptors. However, there no reports of their presence the reproductive tract ram, a seasonal species. Thus, we have investigated existence ram testis, epididymis, accessory glands ductus deferens. Real-time polymerase chain reaction (qPCR) revealed higher levels m-RNA for both receptors ampulla, seminal vesicles, vas deferens, than other organs (p < 0.05). Western blot analyses showed protein bands compatible with testis...
The presence of melatonin receptors on the surface ram spermatozoa has led to speculation about having a role in sperm functionality. aim this study was elucidate mechanism through which regulates capacitation induced by cocktail containing cAMP-elevating agents. Cocktail samples capacitated 1µM showed lower percentages (chlortetracycline staining; P<0.001) together with decrease protein tyrosine phosphorylation (P<0.01) and levels reactive oxygen species (ROS) cAMP (P<0.05) compared without...
To provide new insights into the mechanisms through which seminal plasma proteins (SPP) are able to protect spermatozoa, we tested hypothesis that apoptosis can contribute negative effect of refrigeration on ram and SPP prevent this damage. Having proved presence key constituents apoptosis-related pathways in sperm protein extracts, carried out a comparative analysis effects addition before (15°C, 30 min) induced-apoptosis with betulinic acid or fibroblast-associated receptor ligand,...
It has been proposed that seminal plasma proteins (SPP) support survival of ram spermatozoa, exerting a dual effect, both capacitating and decapacitating. In this study, changes in motility patterns spermatozoa capacitated the presence epidermal growth factor (EGF) were evaluated. Clustering procedures used to determine sperm subpopulations with specific motion characteristics. Four (SP) defined after application principal component analysis procedure. Progressive high straightness (STR)...
Reactive oxygen species (ROS) play an essential role in mammalian sperm capacitation. NADPH oxidase 5 (NOX5) has been described as the main source of ROS production some spermatozoa, such human and equine. On other hand, melatonin can decrease cellular levels regulates NOX activity somatic cells. Therefore, objectives this work were (1) to identify NOX5 ram spermatozoa analyze its possible changes during vitro capacitation (2) investigate effect on expression localization superoxide...
Camels (Camelus dromedarius) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored levels in camel seminal plasma or their relationship with testosterone, age, climatic factors, nor is it known whether receptors exist spermatozoa to respond melatonin. This study aimed analyze its specific spermatozoa. Semen samples were obtained from November March (breeding season). Testosterone measured ELISA. Melatonin localized using...
Administration of melatonin to advance the breeding season in sheep has been widely used, since this hormone conveys photoperiodic signal reproductive neuroendocrine axis. An increased lambing percentage reported following such treatment during anoestrus, which could be mediated through a higher rate embryonic survival, either by an improvement luteal function or reduction antiluteolytic mechanisms. The aim article is review body knowledge on effect ovine ovary, embryo and establishment...
The aim of this study was to assess the effects melatonin on in vitro maturation and fertilization sheep oocytes, culture embryos. Oocytes from ovaries collected at slaughterhouse were divided into four groups, two which treated with either 10E5 M (M5) or 10E6 (M6) melatonin, while other groups served as untreated controls (C5 C6). After fresh ram semen, embryos produced each group sets, one cultured (M5M, C5M, M6M C6M), without (M5C, C5C, M6C, C6C). A concentration increased rate (82.5%...
The present study analysed the involvement of melatonin, acting via its receptors (MT1 and MT2), in ram sperm functionality. Indirect immunofluorescence assays revealed no changes distribution or intensity MT1 receptors, whereas different subpopulations were established for MT2 control, vitro capacitated acrosome-reacted spermatozoa. Chlortetracycline staining following correlations between pattern in: (1) non-capacitated (NC) rate proportion spermatozoa with equal immunostaining acrosome...