A5007347867- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Corneal surgery and disorders
- Intraocular Surgery and Lenses
- Protein Degradation and Inhibitors
- Retinal and Macular Surgery
- Retinal and Optic Conditions
- CRISPR and Genetic Engineering
- Ophthalmology and Eye Disorders
- Retinal Imaging and Analysis
- Photoreceptor and optogenetics research
- Pluripotent Stem Cells Research
- Retinopathy of Prematurity Studies
- Ocular Diseases and Behçet’s Syndrome
- Multiple Myeloma Research and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Photochromic and Fluorescence Chemistry
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Cerebral Venous Sinus Thrombosis
- Traumatic Ocular and Foreign Body Injuries
Moorfields Eye Hospital NHS Foundation Trust
2015-2024
University College London
2015-2024
Moorfields Eye Hospital
2013-2024
NIHR Moorfields Biomedical Research Centre
2013-2023
Verve Therapeutics (United States)
2023
Institute of Ophthalmology
2019-2021
UCL Biomedical Research Centre
2021
National Institute for Health Research
2014-2019
Oregon Health & Science University
2015-2019
Primary Health Care
2019
Primary open angle glaucoma and ocular hypertension are habitually treated with eye drops that lower intraocular pressure. Selective laser trabeculoplasty is a safe alternative but rarely used as first-line treatment. We compared the two.In this observer-masked, randomised controlled trial treatment-naive patients or no comorbidities were recruited between 2012 2014 at six UK hospitals. They randomly allocated (web-based randomisation) to initial selective drops. An objective target pressure...
CRISPR-Cas systems have been broadly embraced as effective tools for genome engineering applications, with most studies to date utilizing the Streptococcus pyogenes Cas9. Here we characterize and manipulate smaller, 1053 amino acid nuclease Staphylococcus aureus Cas9.We find that S. Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM) cleaves target DNA at high efficiency a variety of guide RNA (gRNA) spacer lengths. When directed against genomic targets mutually permissive NGGRRT...
Abstract Müller glia with stem cell characteristics have been identified in the adult human eye, and although there is no evidence that they regenerate retina vivo, can be induced to grow differentiate into retinal neurons vitro. We differentiated cells ganglion (RGC) precursors by stimulation fibroblast growth factor 2 together NOTCH inhibition using γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT). Differentiation RGC was confirmed gene...
PurposeTo report clinical efficacy, predictors of success, and safety primary selective laser trabeculoplasty (SLT) used in treatment-naive patients with open-angle glaucoma (OAG) or ocular hypertension (OHT).DesignPost hoc analysis a multicenter, prospective, randomized, controlled trial.ParticipantsTreatment-naive OAG OHT.MethodsPatients randomized to SLT topical medication treated predefined target intraocular pressures (IOPs) requiring ≥20% IOP reduction from baseline for all disease...
This concise review aims to explore the potential for clinical implementation of artificial intelligence (AI) strategies detecting glaucoma and monitoring progression.Nonsystematic literature using search combinations "Artificial Intelligence," "Deep Learning," "Machine "Neural Networks," "Bayesian "Glaucoma Diagnosis," Progression." Information on sensitivity specificity regarding diagnosis progression analysis as well methodological details were extracted.Numerous AI provide promising...
Abstract Müller glia possess stem cell characteristics that have been recognized to be responsible for the regeneration of injured retina in fish and amphibians. Although these cells are present adult human eye, they not known regenerate vivo. Human with (hMSCs) can acquire phenotypic genotypic rod photoreceptors vitro, suggesting may potential use transplantation strategies treat photoreceptor degenerations. Much work has undertaken rodents using various sources allogeneic restore function,...
The Certificate of Visual Impairment (CVI) provides essential data for preventable sight loss indicators as part the Public Health Outcomes Framework (PHOF) published annually by Department Health. Trends in CVI certification rates can provide information on effectiveness current services and treatments may be used to guide allocation resources, is only such indicator within ophthalmology. This study aimed compare recent trends new vision impairment certifications 2017/18 against prior...
Glaucoma is one of the leading causes blindness, and there an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but a novel sources cells to provide therapeutic benefit. In this study, we have isolated from retinal organoids formed by human induced pluripotent stem (hiPSCs) in vitro shown their ability partially restore visual function rats depleted ganglion NMDA. Based on present results, suggest derived hiPSC attractive...
Abstract. Purpose: To evaluate posterior capsule opacification (PCO) 2 years after cataract surgery following implantation of a hydrophilic or hydrophobic single‐piece acrylic intraocular lens (IOL) with sharp edge. Methods: Phacoemulsification was performed in one eye 120 patients senile this prospective study. They were randomized to either IOL (BL27; Bausch & Lomb, Rochester, NY, USA) (AcrySof ® SA60AT; Alcon Laboratories, Fort Worth, TX, USA). Two surgery, retroillumination images...
Abstract Human Müller glia with stem cell characteristics (hMGSCs) have been shown to improve retinal function upon transplantation into rat models of ganglion (RGC) depletion. However, their translational potential may depend successful engraftment and improvement in experimental anatomical functional features resembling those the human eye. We investigated effect allogeneic feline ability differentiate cells expressing RGC markers, following ablation RGCs by N-methyl-d-aspartate (NMDA)....
MicroRNAs (miRNAs) are small, endogenous noncoding RNAs that have been detected in human aqueous humor (AH). Prior studies pooled samples to obtain sufficient quantities for analysis or used next-generation sequencing. Here, we PCR arrays with preamplification identify and compare miRNAs from individual AH between patients primary open-angle glaucoma (POAG) normal controls.AH was collected before cataract surgery six stable, medically treated POAG eight age-matched controls. Following...
Purpose: MicroRNAs are small, endogenous noncoding RNAs that modulate posttranscriptional gene expression. Although the contribution of microRNAs to pathogenesis glaucomatous damage is unknown, supporting evidence from central nervous system (CNS) research suggests they may play a role. It was therefore hypothesized known be altered in CNS injury also experimental glaucoma. Methods: Intraocular pressure (IOP) elevated rats by unilateral injection hypertonic saline and IOP monitored for 5...
PurposeTo determine the efficacy of repeat selective laser trabeculoplasty (SLT) in medication-naive open-angle glaucoma (OAG) and ocular hypertensive (OHT) patients requiring treatment for early to medium-term failure during Laser Glaucoma Ocular Hypertension (LiGHT) trial.DesignPost hoc analysis SLT arm a multicenter prospective randomized controlled trial.ParticipantsTreatment-naive OAG or OHT 360-degree within 18 months. Retreatment was triggered by predefined IOP disease-progression...
PURPOSE: To evaluate the effect of hydrophilic and hydrophobic single-piece acrylic intraocular lenses (IOLs) on posterior capsule opacification (PCO) after cataract surgery. SETTING: St. Erik's Eye Hospital, Stockholm, Sweden. METHODS: In a prospective study, 120 patients having surgery were randomized to implantation IOL (BL27, Bausch & Lomb) or (AcrySof SA60AT, Alcon). Surgery was performed according standardized protocol by 1 3 surgeons. Best corrected visual acuity, contrast...