A5047054140- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Glycosylation and Glycoproteins Research
- Spectroscopy and Quantum Chemical Studies
- Enzyme Production and Characterization
- Synthesis of Indole Derivatives
- RNA Research and Splicing
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- CRISPR and Genetic Engineering
- Photochemistry and Electron Transfer Studies
- Genetically Modified Organisms Research
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Hematopoietic Stem Cell Transplantation
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Biochemical Analysis and Sensing Techniques
- Peptidase Inhibition and Analysis
- Atomic and Molecular Physics
- Mass Spectrometry Techniques and Applications
Ludwig-Maximilians-Universität München
2004-2023
Urologische Klinik München
2020
Center for Integrated Protein Science Munich
2019
Abstract Background New genomic techniques (NGTs) allow new genotypes and traits to be developed in different ways with outcomes compared previous genetic engineering methods or conventional breeding (including non-targeted mutagenesis). EU GMO regulation requires an assessment of their direct indirect effects that may immediate, delayed cumulative. Such also result from the interactions NGT organisms simultaneously present a shared receiving environment emerge combination traits. This...
Abstract The prototype operation of an ultrafast write–readout–erase–readout cycle all‐optical system based on photochromic indolylfulgides is demonstrated. In the employed dye molecular structure switched using light different wavelengths between two thermally stable states to allow binary encoding information. Non‐destructive readout bit infrared completes scheme memory. For femtosecond pulses are applied and it demonstrated that consecutive write/erase processes separated by less than 4...
Herpes simplex virus 1 (HSV-1) infection exerts a profound shutoff of host gene expression at multiple levels. Recently, HSV-1 was reported to also impact promoter-proximal RNA polymerase II (Pol II) pausing, key step in the eukaryotic transcription cycle, with decreased and increased Pol pausing observed for activated repressed genes, respectively. Here, we demonstrate that induces more complex alterations than previously suspected vast majority cellular genes. While is generally retained,...
Thermally processed food is an important part of the human diet. Heat-treatment, however, promotes formation so-called Amadori rearrangement products, such as fructoselysine. The gut microbiota including Escherichia coli can utilize these compounds a nutrient source. While degradation route for fructoselysine well described, regulation corresponding pathway genes frlABCD remained poorly understood. Here, we used bioinformatics combined with molecular and biochemical analyses show that...
Chemical curiosity or not? Specific antibodies to study the rhamnoproteome in bacteria.
L-Rhamnose is an important monosaccharide both as nutrient source and building block in prokaryotic glycoproteins glycolipids. Generation of those composite molecules requires activated precursors being provided e. g. form nucleotide sugars such dTDP-β-L-rhamnose (dTDP-L-Rha). dTDP-L-Rha synthesized a conserved 4-step reaction which canonically catalyzed by the enzymes RmlABCD. An intact pathway especially for fitness pseudomonads, essential activation polyproline specific translation...
For canonical asparagine glycosylation, the primary amino acid sequence that directs glycosylation at specific residues is well-established. Here we reveal a recently discovered bacterial enzyme EarP, transfers rhamnose to arginine residue in its acceptor protein EF-P, specifically recognizes β-hairpin loop. Notably, while vitro rhamnosyltransferase activity of EarP abolished when presented with linear substrate peptide sequences derived from readily glycosylates same cyclized mimic....
Abstract Thermally processed food is an important part of the human diet. Heat-treatment, however, promotes formation so-called Amadori rearrangement products (ARPs), such as fructoselysine. The gut microbiota including Escherichia coli can utilize these compounds a nutrient source. While degradation route for fructoselysine well described, regulation corresponding pathway genes frlABCD remained poorly understood. Here we use bioinformatics combined with molecular and biochemical analyses...
A brief survey of recent advances in the field coagulation is presented : 1.The active thromboplastin blood due to cooperation between a throm bocyte factor and one plasmatic nature.2. Factor V an inactive substance activated during process form VI which accelerates formation thrombin.3. X helps' convert prothrombin into thrombin presence VI, calcium.The content serum elevated after spontaneous subsides gradually within several days or weeks.Quick's consumption test fails determine serum,...
We report on detailed investigations of the complete ultrafast reaction cycle photochromic indolyl fulgimides observed by combination time-resolved transient absorption and fluorescence studies in UV, VIS, mid-IR spectral range.
Light induced switching of a beta-hairpin structure is investigated by femtosecond IR spectroscopy. While the unzipping process comprises ultrafast kinetics and finished within 1 ns, folding into hairpin much slower process.
Cyclobutane pyrimidine dimers are the major photoproducts formed when DNA is exposed to UV light. Femtosecond time-resolved vibrational spectroscopy shows that thymine in thymidine oligonucleotides < 20 ps after photoexcitation.
Protein N -glycosylation is ubiquitously present in all domains of life, and confers a plethora functions to the protein including increased solubility, protection from degradation, interaction with receptors, activation for function. For canonical asparagine glycosylation, recognition sequence that directs glycosylation at specific residues well-established. It generally holds primary amino acid most important substrate recognition. Here we reveal recently discovered bacterial enzyme called...
<p>Protein <i>N</i>-glycosylation is ubiquitously present in all domains of life, and confers a plethora functions to the protein including increased solubility, protection from degradation, interaction with receptors, activation for function. For canonical asparagine glycosylation, recognition sequence that directs glycosylation at specific residues well-established. It generally holds primary amino acid most important substrate recognition. Here we reveal recently...
Abstract Herpes simplex virus 1 (HSV-1) infection exerts a profound shut-off of host gene expression at multiple levels. Recently, HSV-1 was reported to also impact promoter-proximal RNA Polymerase II (Pol II) pausing, key step in the eukaryotic transcription cycle, with decreased and increased Pol pausing observed for activated repressed genes, respectively. Here, we demonstrate that induces more complex alterations than previously suspected vast majority cellular genes. While is generally...