A5068945405- Cellular transport and secretion
- Sphingolipid Metabolism and Signaling
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Lysosomal Storage Disorders Research
- Pancreatic function and diabetes
National Research Council
2019-2020
Institute of Protein Biochemistry
2019-2020
Abstract The membrane transport apparatus comprises a series of separate bound compartments, or stations, that are responsible for the synthesis, processing, transport, sorting and delivery to their final cellular destinations most transmembrane soluble lumenal proteins. Over last decades system has been shown be extensively regulated both by environmental inputs internal homeostatic signalling systems, control operate maintain homeostasis optimal functionality main such as endoplasmic...
ABSTRACT Sphingolipids have been shown to play important roles in physiology and cell biology, but a systematic examination of their functions is lacking. We performed genome-wide CRISPRi screen sphingolipid-depleted cells identified hypersensitive mutants genes membrane trafficking lipid biosynthesis, including ether synthesis. Systematic lipidomic analysis showed coordinate regulation lipids with sphingolipids, where depletion one these types resulted increases the other, suggesting an...