A5078705486- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Birth, Development, and Health
- Bone Metabolism and Diseases
- Protein purification and stability
- Nanowire Synthesis and Applications
- Bone health and osteoporosis research
- Inflammasome and immune disorders
- Single-cell and spatial transcriptomics
- Diet, Metabolism, and Disease
- Energy and Environment Impacts
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Nutrition, Genetics, and Disease
- Genetic Associations and Epidemiology
- Adipokines, Inflammation, and Metabolic Diseases
- Air Quality and Health Impacts
- vaccines and immunoinformatics approaches
- Exercise and Physiological Responses
- Glycosylation and Glycoproteins Research
- Biosimilars and Bioanalytical Methods
Paul Ehrlich Institut
2021-2025
Tehran University of Medical Sciences
2017-2019
Background Health in early life is crucial for health later life. Exposure to air pollution during embryonic and early-life development can result placental epigenetic modification foetus reprogramming, which influence disease susceptibility Objectives: The aim of this paper was investigate the adaptation level global DNA methylation differential gene expression cycle new-borns exposed high fine particulate matter foetal stage. Study design This a nested case-control study. We enrolled...
Highlights•Bar-coded antibodies distinguish transduced and untransduced T cells by sc-multi-omics•Transduction-resistant upregulate interferon-stimulated genes•Differentially expressed genes are consistent between healthy donors patients•Inhibitors of the ERK signaling pathway increase transduction CAR cell generationSummaryDelivery chimeric antigen receptors (CARs) to is usually mediated lentiviral vectors (LVs), which can have broad tropism or be targeted. To better understand molecular...
Abstract Lentiviral vectors (LV) have become the dominant tool for stable gene transfer into lymphocytes including chimeric antigen receptor (CAR) delivery to T cells, a major breakthrough in cancer therapy. Yet, room improvement remains, especially latest LV generations delivering genes selectively cell subtypes, key requirement vivo CAR generation. Toward improving rates with these vectors, whole transcriptome analyses on human are conducted after exposure CAR‐encoding conventional...
The aim of the study was to investigate association between high-density lipoprotein (HDL) and bone status taking into account serum vitamin D levels in postmenopausal Iranian women.During January 2015 February 2016, a total 488 women participated this cross-sectional study, all whom were not osteoporosis medication suffering from any chronic disorder. Dual X-ray absorptiometry used assess mineral density (BMD) hip, femoral neck, lumbar vertebrae (L2-L4). Each person categorized based on...
Background: Cancer immunotherapy is a promising strategy for cancer treatment. In this strategy, the immune system triggered to destroy cells. IL-2 an important factor in passive that helps modulating some functions. One of limitations low serum half-life; therefore, repetitive high doses injections are required maintain effective concentrations. High-dose therapy results severe side effects; thus, improvement its half-life would provide therapeutic benefits.We have investigated able utilize...
Quantifying gene expression in individual cells can substantially improve our understanding about complex genetically engineered cell products such as chimeric antigen receptor (CAR) T cells. Here we designed a single-cell RNA sequencing (scRNA-seq) approach to monitor the delivery of CD19-CAR via lentiviral vectors (LVs), i.e., conventional vesicular stomatitis virus (VSV)-LV and CD8-targeted CD8-LV. LV-exposed human donor peripheral blood mononuclear (PBMCs) were evaluated for panel 400...
Interferonbeta-1b (IFNβ-1b) developed as therapeutic protein for the treatment of multiple sclerosis (MS). Studies have been shown that Long-term usage this can lead to development anti-drug antibodies (ADAs) and phenomenon cause total loss or reduced efficacy IFNβ-1b. The aim study was predict silence IFNβ-1b T-cells epitopes by in silico methods genetic engineering. Based on bioinformatics studies we identified optimal sets conservative point mutations eliminating protein. Four synthetic...
<h3>Background</h3> Cytokine release syndrome (CRS) is a serious concern in chimeric antigen receptor (CAR) T cell therapy. Rapidly produced CAR cells are new format for which CRS occurrence so far hardly studied. Animal models needed. Here, we investigate the relationship between short-term (stCART) and of using NSG-SGM3 mice. <h3>Methods</h3> In three different setting, stCART potency triggering was investigated. First, CD19-specific were compared to activated PBMC CD19+ tumor engrafted...
<h3>Background</h3> CRS (Cytokine Release Syndrome) is considered as a recurrent side effect of CAR (Chimeric Antigen Receptor) T cell therapy. An experimental animal model for better investigating different aspects still in demand. Besides cells` astonishing achievements, numerous efforts are determined on manufacturing hindrance, notably shortening production procedures. Here, we introduce novel mouse employing the NSG-SGM3 strain to address adverse effects FT- cells (Fast-Track T)....
Summary Chimeric antigen receptor (CAR) T cells have emerged as effective strategy against B cell malignancies. Since the long manufacturing process limits patient accessability, short-term (st) CAR are under investigation. Here, we evaluated CD19-CAR 24 hours after exposure to lentiviral vectors. In co-culture with tumor and monocytes, stCAR exhibited anti-tumoral activity strong release of CRS-relevant cytokines (IL-6, IFN-γ, TNF-α, GM-CSF, IL-2, IL-10). When administered into engrafted...