A5026414977- Antioxidant Activity and Oxidative Stress
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Agricultural Practices and Plant Genetics
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Resistance
- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Postharvest Quality and Shelf Life Management
- Lung Cancer Treatments and Mutations
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Phytochemicals and Antioxidant Activities
- Cancer Research and Treatments
- Radiopharmaceutical Chemistry and Applications
- Legume Nitrogen Fixing Symbiosis
- Cancer-related Molecular Pathways
- Greenhouse Technology and Climate Control
- Cancer, Hypoxia, and Metabolism
- Leaf Properties and Growth Measurement
- Spectroscopy and Chemometric Analyses
The Ohio State University
2016-2025
Chiang Mai University
2023
Ohio Department of Agriculture
2001-2021
University of Wisconsin Carbone Cancer Center
2020
University of Wisconsin–Madison
2020
Sejong University
2015-2017
Sokoine University of Agriculture
2016
Ministry of Agriculture, Food Security and Cooperatives
2016
Michigan State University
2015
North Carolina State University
2006-2015
Abstract Phenotypic diversity within cultivated tomato (Solanum lycopersicum) is particularly evident for fruit shape and size. Four genes that control have been cloned. SUN OVATE elongated whereas FASCIATED (FAS) LOCULE NUMBER (LC) locule number flat shape. We investigated the distribution of alleles in germplasm evaluated their contribution to morphology a diverse collection 368 predominantly var. cerasiforme accessions. Fruits were visually classified into eight categories supported by...
The concurrent development of high-throughput genotyping platforms and next generation sequencing (NGS) has increased the number density genetic markers, efficiency constructing detailed linkage maps, our ability to overlay recombination physical maps genome. We developed an array for tomato with 8,784 Single Nucleotide Polymorphisms (SNPs) mainly discovered based on NGS-derived transcriptome sequences. Of SNPs, 7,720 (88%) passed manufacturing quality control could be scored in germplasm....
Domestication modifies the genomic variation of species. Quantifying this provides insights into domestication process, facilitates management resources used by breeders and germplasm centers, enables design experiments to associate traits with genes. We described analyzed genetic diversity 1,008 tomato accessions including Solanum lycopersicum var. (SLL), S. cerasiforme (SLC), pimpinellifolium (SP) that were genotyped using 7,720 SNPs. Additionally, we explored allelic frequency six loci...
Scope Tangerine tomatoes ( Solanum lycopersicum ) are rich in tetra‐ cis ‐lycopene resulting from natural variation carotenoid isomerase. Our objective was to compare the bioavailability of lycopene tangerine red tomato juice, and elucidate physical deposition forms these isomers by light electron microscopy. Methods results Following a randomized cross‐over design, subjects n = 11, 6 M/5 F) consumed two meals delivering 10 mg (94% or juice (10% ). Blood sampled over 12 h triglyceride‐rich...
Lycopene is present mainly as cis -isomers in human serum and tissues whereas all- trans -lycopene predominates tomato products, suggesting that isomerised the body or less bioavailable. The objectives of study were to develop processing conditions for tomatoes obtain products with different - isomer distribution assess their bioavailability. Healthy adult subjects ( n 12) recruited this randomised cross-over trial. Each intervention was preceded by a 2-week washout period. Two sauces, one...
The effects of selection on genome variation were investigated and visualized in tomato using a high-density single nucleotide polymorphism (SNP) array. 7,720 SNPs genotyped collection 426 accessions (410 inbreds 16 hybrids) over 97% the markers polymorphic entire collection. Principal component analysis (PCA) pairwise estimates F(st) supported that inbred represented seven sub-populations including processing, large-fruited fresh market, vintage, cultivated cherry, landrace, wild S....
The physiological traits underlying the apparent drought resistance of 'Tomàtiga de Ramellet' (TR) cultivars, a population Mediterranean tomato cultivars with delayed fruit deterioration (DFD) phenotype and typically grown under non-irrigation conditions, are evaluated. Eight different accessions were selected included six TR accessions, one non-TR accession (NTR(M)) processing cultivar (NTR(O)). Among two leaf morphology types, normal divided leaves potato-leaf, selected. Plants field...
Measuring fruit morphology and color traits of vegetable crops in an objective reproducible way is important for detailed phenotypic analyses these traits. Tomato Analyzer (TA) a software program that measures 37 attributes related to two-dimensional shape semi-automatic manner1,2. Many attributes, such as angles at the distal proximal ends areas indentation, are difficult quantify manually. The organized ten categories within software: Basic Measurement, Fruit Shape Index, Blockiness,...
Ty-6 is a major resistance gene on chromosome 10 of tomato that provides against monopartite and bipartite begomoviruses complements conferred by the known Ty-3 ty-5 genes. Resistance to an important breeding objective for cultivated tomato. Several begomovirus genes have been introgressed from related Solanum species are available purposes. In present study, we mapped additional locus, Ty-6, effective both Tomato yellow leaf curl virus (TYLCV) mottle (ToMoV). Gene action incomplete...
Measuring plant characteristics via image analysis has the potential to increase objectivity of phenotypic evaluations, provides data amenable quantitative analysis, and is compatible with databases that aim combine genotypic data. We describe a new tool, which implemented in Tomato Analyzer (TA) software application, called Color Test (TACT). This tool allows for accurate quantification color uniformity, scanning devices be calibrated using standards. To test accuracy precision TACT, we...
Plant breeding is enhanced by the availability of molecular markers for rapid screening and selection in populations. Identification polymorphic loci cultivated tomato (Solanum lycopersicum L.) has been hampered limited genome sampling across types. Whole transcriptome sequencing six accessions that span market classes was performed using synthesis. A total 291,915,037 quality filtered reads representing 17 Gb sequence were generated. Assembly resulted 30.6 to 34.9 Mb each provided...
The history of tomato (Solanum lycopersicum L.) improvement includes genetic bottlenecks, wild species introgressions, and divergence into distinct market classes. This makes an excellent model to investigate the effects selection on genome variation. A combination linkage mapping in two F2 populations physical with emerging sequence data was used position 434 PCR-based markers including SNPs. Three-hundred-and-forty were genotype 102 lines representing species, landraces, vintage cultivars,...
Prolonged tomato consumption can mitigate ultraviolet (UV) light induced sunburn via unknown mechanisms. Dietary carotenoids distributed to skin are hypothesized protect against UV-induced damage, although other phytochemicals may play a role. We hypothesize that would cancer. SKH-1 hairless and immunocompetent mice (n = 180) were fed AIN-93G or + 10% tangerine red powder for 35 weeks. From weeks 11-20, 120) exposed 2240 J/m2 UV-B light, 3x/week, tumors tracked weekly. Control the same diets...
Abstract The susceptibility of predominant tomato carotenoids to thermal isomerisation during typical food preparation is reported for five varieties with distinctively different carotenoid distribution. used contain distinct amounts the following carotenoids: all‐ trans lycopene, β‐carotene, δ‐carotene, lutein and poly‐ cis geometrical isomer prolycopene. tomatoes were subjected treatments unit operations similar those preparation: boiling, addition vegetable cooking oil, chopping...
We used molecular markers to identify quantitative trait loci (QTL) that contribute resistance bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis. Resistance was first identified as a marker-trait association in an inbred backcross (IBC) population derived from crossing Lycopersicon hirsutum accession (LA407) with L. esculentum. Single-marker QTL analysis suggested at least two originating LA407, Rcm 2.0 on chromosome 2 and 5.1 5, replicated trials. Two...
SUMMARY The molecular basis of non‐host resistance, or species‐specific remains one the major unknowns in study plant–microbe interactions. In this paper, we describe characterization a pathosystem involving model plant Arabidopsis thaliana and economically important destructive oomycete pathogen Phytophthora infestans . Cytological investigations into early stages interaction revealed germination P. cysts on leaves, direct penetration epidermal cells, formation infection vesicles...
ABSTRACT: Lycopene, a tomato carotenoid, has been associated with the inhibition of certain chronic diseases including prostate cancer. Tomato oleoresin is lipid‐rich material resulting from successive solvent extraction fruit. Thermal stability and isomerization lycopene in oleoresins prepared 3 different varieties, Roma, High Tangerine, peel waste, were studied at 25 °C, 50 75 100 °C dark. Thermally degraded compounds isomers analyzed by combination C 30 reversed‐phase high‐performance...
ABSTRACT Clavibacter michiganensis subsp. sepedonicus is a plant-pathogenic bacterium and the causative agent of bacterial ring rot, devastating agricultural disease under strict quarantine control zero tolerance in seed potato industry. This organism appears to be largely restricted an endophytic lifestyle, proliferating within plant tissues unable persist absence material. Analysis genome sequence C. comparison with sequences related pathogens revealed dramatic recent evolutionary history....
Tomato sauces were produced from unique tomato varieties to study carotenoid absorption in humans. Tangerine tomatoes, high cis-lycopene, especially prolycopene (7Z,9Z,7'Z,9'Z), and high-β-carotene tomatoes as an alternative dietary source of β-carotene grown processed. Sauces served after 2 week washout periods overnight fasting for breakfast healthy subjects (n = 12, 6M/6F) a randomized crossover design. The serving size was 150 g (containing 15 corn oil), tangerine sauce containing 13 mg...
The rapid quantification of lycopene and beta-carotene in tomato juices by attenuated total reflectance (ATR) infrared spectroscopy combined with multivariate analysis was evaluated. Two sample preparation methods were compared: a direct measurement the paste an extraction method using hexane to isolate carotenoids. HPLC used as reference method. Cross-validated (leave-one-out) partial least-squares regression (PLSR) create calibration models predict these phytonutrient concentrations blind...
A study was conducted to characterize the storage stability of lycopene in hot-break tomato juice prepared from two different cultivars and processed by various pressure−heat combinations. Samples were subjected pressure assisted thermal processing (PATP; 600 MPa, 100 °C, 10 min), high (HPP; 700 45 (TP; 0.1 35 min). Processed samples stored at 4, 25, 37 °C for upto 52 weeks. HPP PATP treatments significantly improved extractability over TP control. All-trans found be fairly stable...